Centrifugal micro-fluidic chip and exosome extraction method

Abstract

The invention relates to a centrifugal micro-fluidic chip and an exosome extraction method. The centrifugal micro-fluidic chip comprises a rotation center and at least one extraction mechanism, and the extraction mechanism comprises a sample injection unit, a capture and release unit and an enrichment unit; the sample injection unit is used for inputting and storing sample liquid; the capturing and releasing unit is connected with the sample injection unit, is positioned at the downstream of the sample injection unit and is used for capturing or releasing an extraction target object in sampleliquid; and the enrichment unit is connected with the capture and release unit, is positioned at the downstream of the capture and release unit and is used for enriching the extracted target object released by the capture and release unit. By utilizing the centrifugal micro-fluidic chip, the purpose of rapidly extracting the exosome can be achieved by only adding a sample once and controlling thechip to rotate, the operation is simple and convenient, the extraction speed is high, the efficiency is high, the reagent consumption is low, and the amount of the extracted exosome is large.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a centrifugal microfluidic chip and an exosome extraction method. Background technique [0002] Exosomes are extracellular nanoscale vesicles formed by cells through the process of "endocytosis-fusion-efflux". The size is between 50nm and 150nm. They are high in blood and have the ability to transport nucleic acids across the blood-brain barrier ( The properties and functions of biologically active molecules such as miRNA), proteins and lipids. Exosomes, as a carrier for communicating and transferring substances between cells, play a regulatory role in recipient cells, and have been found to play a vital role in a series of physiological and pathological processes. Exosomes are currently extracted mainly from body fluids such as blood, saliva, urine, cerebrospinal fluid, semen, saliva, pleural effusion, and milk. [0003] Exosome extraction methods mainly include ultracen...

AI Technical Summary

Problems solved by technology

However, using the above method to extract exosomes usually requires multiple sample additions and sample changes, which has the disadvantages of long time-consuming and cumbersome operation.

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