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Spruce seed surface sterilization method and culture dish for sterilization

A surface sterilization and petri dish technology, applied in the direction of seed immunity, can solve the problems of difficult testing, reducing the sterilization effect, and increasing the contamination of seeds by miscellaneous bacteria.

Active Publication Date: 2021-03-19
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such an operation cannot completely eliminate the microorganisms on the surface of the seeds, and the residual harmful microorganisms may also affect the germination of the seeds
[0003] At the same time, the existing sterilization equipment (petri dishes, etc.) needs to be manually operated in batches, and it is difficult to conduct a batch of tests at the same time. The batch operation also increases the possibility of seeds being contaminated by bacteria and reduces the sterilization effect

Method used

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  • Spruce seed surface sterilization method and culture dish for sterilization
  • Spruce seed surface sterilization method and culture dish for sterilization
  • Spruce seed surface sterilization method and culture dish for sterilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0051] When the culture dish has three layers, generally the liquid in the liquid layer 80 is discharged first, and then the liquid in the treatment layer 50 is discharged; that is, the second liquid circulation structure is activated first, and then the first liquid circulation structure is activated. In the case where only one central column 20 is provided, there are many ways to achieve the above purpose. The second spline 22 of the central column 20 in the second liquid circulation structure can be set to have the same shape as the first spline 21, and the central opening communicating with the drain plate 60 also has the same shape and size as the central opening communicating with the waste drain plate 30. Similarly, when the central column 20 is rotated, the liquid-communicating drain plate 60 and the waste-discharging plate 30 rotate simultaneously. Set each waste discharge hole and liquid discharge hole in sequence, and when it is rotated in one direction at a certain...

Embodiment

[0059] 1. On October 20, 2019, 1 kg of mature spruce seeds were collected in Lixian County. The harvested spruce seeds were soaked in a glass beaker filled with tap water. After 20 hours, the seeds floating on the surface and impurities in the water were removed.

[0060] 2. Air-dry the seeds: Dry the rinsed spruce seeds in the shade at room temperature, put them in bags, and transfer them to a refrigerator at 4°C for storage.

[0061] 3. Vitality determination: prepare a concentration of 0.75% TTC (2,3,5-triphenyltetrazolium chloride) solution, peel the spruce seeds and use a scalpel to excise a small part of the seed tissue (including cotyledon, etc.), then soaked in a new TTC solution with a concentration of 0.75%, reacted for 2 hours under 30°C in the dark, and observed the vigor of the seeds under a stereomicroscope. If the radicle and more than half of the cotyledons were dyed red, it was regarded as positive. Seeds of vitality.

[0062] 4. Verification of germination: ...

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Abstract

The invention belongs to the field of seed treatment, and particularly relates to a spruce seed surface sterilization technology. According to the specific technical scheme, 20%-30% of w / v H2O2 solution is used for soaking seeds for 5-30 min; and a specific culture dish is used for carrying out sterilization operation, wherein the culture dish at least comprises two layers, one layer is a treatment layer capable of containing the seeds to be sterilized, and liquid circulation can be carried out between the layers in a controllable manner. The invention provides the method for sterilizing the surfaces of the spruce seeds for the first time, and the sterilization effect is as high as 100%; The invention also provides a seed germination method based on the method, and the highest germinationrate is up to 100%. In order to carry out sterilization more conveniently and accurately, the invention also provides the culture dish which can be used for carrying out multi-gradient and multi-repeated sterilization tests at the same time, so that the sterilization effect is effectively improved.

Description

technical field [0001] The invention belongs to the field of seed treatment, and in particular relates to a surface sterilization technology of spruce seeds. Background technique [0002] In order to study the effect of spruce seed endophytes on seed germination and seedling colonization, the first problem to be solved is to obtain seed endophytes by sterilizing the microorganisms on the seed surface. However, due to the limitation of seed surface sterilization technology, the same sterilizing agent, sterilization concentration and sterilization time will have different sterilization effects on microorganisms on different seed surfaces. For spruce seeds, 0.3% K is currently used more 2 MnO 4 Soak in the solution for 15 minutes for simple disinfection. However, such an operation cannot completely eliminate the microorganisms on the surface of the seeds, and the residual harmful microorganisms may also affect the germination of the seeds. [0003] At the same time, the exi...

Claims

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Application Information

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IPC IPC(8): A01C1/08
CPCA01C1/08
Inventor 尹春英唐小乐刘庆华
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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