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Propagule extract and application thereof

A technology of propagules and extracts, which is applied in the fields of agriculture, chemistry and biology, can solve the problems of environmental hazards, and has not yet developed a propagule dormancy prolonging agent, so as to achieve the effects of inhibiting respiration, effectively prolonging the dormancy period, and promoting reproduction

Pending Publication Date: 2021-12-17
沈阳恩柽研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problem that the propagule dormancy prolonging agent has environmental hazards in the prior art or some propagule dormancy prolonging agents have not been developed, and provides a kind of original ecology propagule dormancy prolonging extract and its use

Method used

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  • Propagule extract and application thereof
  • Propagule extract and application thereof
  • Propagule extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0185] Embodiment 1, peanut dormancy extension extract

[0186] Selection of peanut materials: Select the same batch of planted and unearthed in the previous year, mature and plump dried peanuts with shells of the four-red variety, and carefully separate the peanut shells and peanut kernels.

[0187] Take 5kg of peanut shells, crush them, add water at a ratio of 1g: 10mL, soak and extract at room temperature and avoid light for 24h, after 24h, ultrasonicate at 50°C for 1.5h, filter, and filter under reduced pressure to obtain the filtrate; Concentrate and freeze-dry to obtain the crude extract of peanut dormancy extension.

[0188] The crude extract is further processed subsequently: after adding 75L deionized water to the crude extract for dissolving, then purify through the AB-8 macroporous resin column, the conditions are: sample loading temperature 25°C, sample loading flow rate 1BV / h, sample loading The volume is 5BV; the eluent is aqueous solution, the elution temperatu...

Embodiment 2

[0202] Embodiment 2, soybean dormancy extension extract

[0203] 1. Test materials and instruments

[0204] Test materials: (this experiment was carried out in March), the soybeans to be tested were collected from Fushun, Liaoning. Among soybeans sown in the same batch, harvested in the same batch, and with similar maturity, soybeans without pests and mechanical damage were selected.

[0205] Test equipment: medicinal material pulverizer, analytical balance, ultrasonic oscillator, centrifuge, rotary evaporator, etc.

[0206] 2. Test method

[0207]Select dry soybean stalks and pods for crushing, and add absolute ethanol at a ratio of 1g:7mL. After sealing and refrigerating for 24 hours, vibrate with an ultrasonic oscillator at 30°C for 1.5 hours, retain the filtrate after suction filtration, distill the filtrate under reduced pressure until there is no fraction, and freeze-dry to obtain the crude extract of soybean dormancy extension.

[0208] The soybean dormancy prolongin...

Embodiment 3

[0217] Embodiment 3, grape dormancy extension extract

[0218] 1. Test materials and instruments

[0219] Test materials: the tested Vitis vinifera clones were collected from Yingkou, and the grape branches with smooth surface, no pests, no mechanical damage, and not yet germinated were selected.

[0220] Test equipment: medicinal material pulverizer, analytical balance, ultrasonic oscillator, centrifuge, rotary evaporator, etc.

[0221] 2. Test method

[0222] The vine branches are washed with water, dried in the air, crushed, and a crude extraction solvent is added in a ratio of 1 g: 8 mL, and the crude extraction solvent is sterile water. After sealing and refrigerating for 24 hours, vibrate with an ultrasonic oscillator at 50°C for 0.5-1.5 hours, retain the filtrate after suction filtration, distill the filtrate under reduced pressure until there is no fraction, and freeze-dry to obtain the crude extract of grape dormancy extension.

[0223] The crude extract of Vitis v...

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Abstract

The invention discloses a propagule (including individual, organ, tissue, cell and other biological sexual, asexual and parthenogenetic propagation materials, maternal, young, ovum and germ cells, and animal tissue, cell, spore, hypha, sporocarp and other sexual or asexual propagation fungus materials for division reproduction, germination reproduction, rupture reproduction and the like) extract and an application thereof. The propagule extract is obtained by extracting a required propagule; when in use, the extract is uniformly mixed with a solvent to prepare a dormancy period prolonging agent. The surface of the propagule can be covered with a storage life prolonging agent to form a film by means of spraying, soaking, brushing and the like, the treated propagule can be stored by means of packaging, sustained-release microcapsules and the like after being aired, or the storage life prolonging agent is combined with other storage methods to prolong the dormancy period. According to the propagule (including the individual, the organ, the tissue, the cell and the other biological sexual, asexual and parthenogenetic propagation materials, the maternal, the young, the ovum and the germ cells, and the animal tissue, the cell, the spore, the hypha, the sporocarp and the other sexual or asexual propagation fungus materials for division reproduction, germination reproduction, rupture reproduction and the like) extract and the application thereof, The dormancy period of the propagule can be obviously prolonged, the reproductive capacity of the propagule subjected to activating treatment is recovered, and the development of the subsequent propagule is not influenced.

Description

technical field [0001] The invention belongs to the fields of biology, agriculture and chemistry, and in particular relates to a propagule (any biological material that can be used for the purpose of reproduction) to prolong the dormant period (refers to the individual, organ, meristematic tissue and Cells, growth and metabolism temporarily suspended, life activity significantly reduced) extract and application thereof. Background technique [0002] Dormancy refers to the phenomenon that individuals, organs, meristems and cells with growth ability cannot start to grow under suitable conditions (Rohde A, Bhalerao RP. Plant dormancy in the perennial context[J]. Trends in Plant Science, 2007,12(5):217~223.). [0003] The dormancy period refers to the period in which the growth and metabolism of individuals, organs, meristems and cells with the ability to grow temporarily stop and life activities are significantly reduced. [0004] Prolongation of the dormant period refers to ...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01G7/06A01K67/02A01K67/033A01N3/00A01N1/02
CPCA01C1/00A01G7/06A01K67/02A01K67/033A01N3/00A01N1/0226
Inventor 李世友赵福强韩月泠刘刚刘琳陈恒雪
Owner 沈阳恩柽研究院有限公司
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