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A method for detecting endotoxin concentration in biochemical tail water by using nanotube membrane pressure difference

A nanotube membrane and endotoxin technology, applied in the field of environmental pollution monitoring, can solve the problems of expensive equipment, no shielding ability, large volume, etc., and achieve the effect of improving accuracy and reducing errors

Active Publication Date: 2021-08-13
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a fast detection speed and does not consume detection reagents, but it needs to rely on expensive equipment and a very large laser particle size analyzer, and it has no shielding ability to various interference factors in water, so there are many problems in the actual application process
Other methods have more exploratory research, but have not yet been applied, or cannot overcome the shortcomings of adding reagents or using expensive instruments, and cannot achieve direct detection of samples

Method used

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  • A method for detecting endotoxin concentration in biochemical tail water by using nanotube membrane pressure difference
  • A method for detecting endotoxin concentration in biochemical tail water by using nanotube membrane pressure difference
  • A method for detecting endotoxin concentration in biochemical tail water by using nanotube membrane pressure difference

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for detecting endotoxin concentration in biochemical tail water by using nanotube membrane pressure difference, comprising the following steps:

[0028] Firstly, the platinum nanotube membrane is prepared, and the polycarbonate porous membrane with uniformly distributed nanopores and a pore size of 5 nm is fixed on the membrane and plated with silver in a physical vapor deposition vacuum system, and then the polycarbonate porous membrane is coated with potassium chloroplatinate. Plating by chemical method, and then releasing the polycarbonate porous membrane with dichloromethane to obtain the completed platinum nanotube membrane for subsequent membrane cell assembly, and then clamping and fixing the platinum nanotube membrane with double-sided support layers In the flat nanofiltration membrane tank, the upper plate water inlet channel, the upper plate flow channel and the upper plate water outlet channel are arranged in the flat nanofiltration membrane tank, and...

Embodiment 2

[0033] The endotoxin in the secondary biochemical effluent of a sewage treatment plant in Beijing was measured by the method in Example 1. Since the water sample contained more suspended particles, the water sample was stored at 4°C after collection After 24 hours, the particles were completely precipitated and processed. The HLB column was used to purify the water sample. After removing the heat source interference, the peristaltic pump was injected into the membrane pool at a flow rate of 0.1mL / min, and the measured transmembrane pressure difference Compared with the endotoxin concentration measured by LAL method, the results are as follows (see Figure 4 ):

[0034]

Embodiment 3

[0036] The method in Example 1 is used to measure the endotoxin in the secondary biochemical effluent of a sewage treatment plant in Beijing. Other operating methods are similar to Example 2, and will not be repeated. The measured transmembrane pressure difference is compared with that measured by the LAL method. The endotoxin concentration was compared, and the results are as follows (see Figure 4 ):

[0037]

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Abstract

The invention relates to the field of environmental pollution monitoring, in particular to a method for measuring endotoxin in biochemical tail water through the transmembrane pressure difference of nanotube membranes. The invention comprises the steps of preparing a platinum nanotube array with a regular arrangement, and preparing a micro-membrane module after stripping, washing, purifying, and substrate; purifying the biochemical tail water through an HLB column, and filtering it through an ultrafiltration membrane , mixed with LAL reagent and continuously injected into the nanotube membrane module, measured the stable membrane pressure difference for a specified period of time, converted it into an electrical signal, and established a standard curve to calculate the endotoxin concentration. The present invention converts the transmembrane pressure into an electrical signal to measure the nanoparticles formed by endotoxin and Limulus reagent, which reduces the error of manual visual inspection; in addition, the method can reduce the amount of Limulus reagent by more than 20 times, significantly reducing the single inspection cost and testing time.

Description

technical field [0001] The invention relates to the technical field of environmental pollution monitoring, in particular to a method for measuring endotoxin in biochemical tail water through the transmembrane pressure difference of nanotube membranes. Background technique [0002] In recent years, the amount of reclaimed water has been increasing year by year, and the endotoxin problem in reclaimed water has aroused widespread concern in the environmental and medical circles. Endotoxins are lipopolysaccharide (LPS) components located in the cell wall of Gram-negative bacteria such as E. coli and released from the cell into the environment during cell division or death. Since the biochemical process involved in sewage treatment is a process in which microorganisms proliferate in large numbers, it inevitably leads to the release of endotoxin from microbial cell rupture. [0003] The vast majority of urban reclaimed water comes from the secondary and tertiary biochemical efflu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/18
CPCG01N33/1826
Inventor 殷立峰代云容张圆正刘双段存戌
Owner BEIJING NORMAL UNIVERSITY
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