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Preparation method of nanomotor-hydrogel system for gene-targeted therapy

A targeted therapy and hydrogel technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problem of difficulty in entering cells, achieve low cytotoxicity, good gene targeted therapy effect, and increase the effect of retention time.

Active Publication Date: 2022-02-25
CHINA UNIV OF MINING & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, naked small interfering RNA is difficult to enter cells due to its hydrophilicity and electronegativity

Method used

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  • Preparation method of nanomotor-hydrogel system for gene-targeted therapy
  • Preparation method of nanomotor-hydrogel system for gene-targeted therapy

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preparation example Construction

[0026] A preparation method of an injectable high cell uptake nanomotor-hydrogel system for gene-targeted therapy of the present invention, comprising the following steps:

[0027] Step 1. First drop the platinum nanoparticle solution on the substrate and dry it;

[0028] Step 2. Then soak the substrate in polyethyleneimine solution, take out the substrate, wash it with ultrapure water several times, then soak the substrate in sodium polystyrene sulfonate solution, take out the substrate, and wash it with ultrapure water repeatedly;

[0029] Step 3. Repeat step 2 repeatedly, assemble polyethyleneimine and sodium polystyrene sulfonate on the surface of platinum nanoparticles, and assemble folic acid-modified polyethyleneimine on the outermost layer to obtain nanocarriers;

[0030] Step 4. Use ultrasound to disperse the nano-carrier in mold-free sterile water, and connect the small molecule interfering RNA to the nano-carrier through electrostatic interaction to obtain a nanomo...

Embodiment 1

[0034] Drop 3 mg / mL platinum nanoparticle solution on the substrate and dry it; then soak the substrate in 3 mg / mL polyethyleneimine solution for 5 min, take out the substrate, wash it with ultrapure water for 3 times, and then put The substrate was soaked in 3 mg / mL sodium polystyrene sulfonate solution for 5 min, the substrate was taken out, and washed 3 times with ultrapure water; the steps were repeated 15 times to assemble polyethyleneimine and polystyrene sulfonate on the surface of platinum nanoparticles. Sodium acid, and folic acid-modified polyethylenimine was assembled on the outermost layer to obtain nanocarriers; the nanocarriers were dispersed in mold-free sterile water (concentration 3 mg / mL) by ultrasound, and mixed with 5 mM small interfering RNA in React in an ice bath for 1 hour to obtain nanomotors; mix 40 mg / mL chitosan solution and 17 mg / mL dialdehyde starch solution (containing 3 mg / mL nanomotors) to prepare an injectable high Cellular uptake of nanomotor...

Embodiment 2

[0038] Drop 1 mg / mL platinum nanoparticle solution on the substrate and dry it; then soak the substrate in 2 mg / mL polyethyleneimine solution for 5 min, take out the substrate, wash it with ultrapure water for 3 times, and then put The substrate was soaked in 2 mg / mL sodium polystyrene sulfonate solution for 5 min, the substrate was taken out, and washed 3 times with ultrapure water; the steps were repeated 10 times to assemble polyethyleneimine and polystyrene sulfonate on the surface of platinum nanoparticles. Sodium acid, and folic acid-modified polyethylenimine was assembled on the outermost layer to obtain nanocarriers; the nanocarriers were dispersed in mold-free sterile water (concentration 3 mg / mL) by ultrasound, and mixed with 5 mM small interfering RNA in React in an ice bath for 1 hour to obtain nanomotors; mix 40 mg / mL chitosan solution and 20 mg / mL dialdehyde starch solution (containing 3 mg / mL nanomotors) to prepare an injectable high Cellular uptake of nanomotor...

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Abstract

The present invention is a preparation method of an injectable high cell uptake nanomotor-hydrogel system for gene targeted therapy. The platinum nanoparticle solution was dropped on the substrate and dried; the substrate was soaked in the polyethyleneimine solution, the substrate was taken out, washed with ultrapure water for several times, the substrate was soaked in the sodium polystyrene sulfonate solution, and the The substrate was taken out and washed with ultrapure water for several times; the above steps were repeated several times, polyethyleneimine and sodium polystyrene sulfonate were assembled on the surface of platinum nanoparticles, and folic acid-modified polyethyleneimine was assembled on the outermost layer; ultrasonic waves were used The nano-carrier is dispersed in mildew-free sterile water, and the small molecule interfering RNA is connected to the nano-carrier through electrostatic action to obtain a nano-motor; the chitosan solution and the dialdehyde starch solution containing the nano-motor are mixed to complete the preparation. The invention improves cell uptake performance and cell retention time, and achieves better gene targeted therapy effect. The method of the invention is simple and effective, the operation is simple and the time required is short.

Description

technical field [0001] The invention relates to the technical field of gene targeting therapy, in particular to a preparation method of an injectable high cell uptake nanomotor-hydrogel system for gene targeting therapy. Background technique [0002] According to the International Agency for Research on Cancer, cancer has the potential to become the leading cause of death worldwide in the 21st century. Today, the main treatments for cancer include surgery, radiation therapy, and chemotherapy. But these therapies have limitations, such as residual tumor cells and drug resistance after surgery. Gene therapy is one way to address these problems. Small interfering RNA is a commonly used therapeutic agent in gene therapy. It reduces gene expression leading to apoptosis. However, naked small interfering RNA is difficult to enter cells due to its hydrophilicity and electronegativity. Therefore, various nanocarriers have been developed for transporting small interfering RNAs. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7088A61K9/06A61K47/32A61K47/36A61K47/54A61K47/02A61P35/00B82Y5/00
CPCA61K9/06A61K9/0024A61K31/7088A61K47/32A61K47/36A61K47/02A61K47/545A61P35/00B82Y5/00
Inventor 任姣雨王虹
Owner CHINA UNIV OF MINING & TECH
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