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Stem cell culture medium with high amplification efficiency

An amplification efficiency and stem cell technology, applied in the field of stem cell culture medium, can solve the problems of general expansion efficiency and high cost of stem cell culture medium, and achieve the effects of good cell uniformity, high amplification efficiency and high purity

Pending Publication Date: 2021-03-09
无锡华泰创新药技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The embodiment of the present invention provides a stem cell culture medium with high expansion efficiency, aiming to solve the problems of high cost and general expansion efficiency of the existing stem cell culture medium

Method used

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  • Stem cell culture medium with high amplification efficiency
  • Stem cell culture medium with high amplification efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027]A stem cell medium having high amplification efficiency, composed of an EM medium and an adding component including: sodium selenate having a concentration of 30 ng / ml, sodium concentration of 10 ng / ml, Insulin having a concentration of 15 μg / ml, a protein having a concentration of 1 μg / ml of a concentration of 1 ng / ml of a fibroblast growth factor, a concentration of 0.1 ng / ml epidermal growth factor, a concentration of 5 ng / ml vitamin and reduction Antiococeransidase III of type and concentration of 0.1 ng / ml, which adds a concentration of 0.8 g / dl of globulin and 2 g / dl albumin, the vitamin and reductive material including D-flue-ingotonic calcium , Folic acid, nicotinamide, pyridoxine, riboflavin, vitamin B35, vitamin B60, vitamin B12, inositol, glycine, L-alanine, L-arginine, L-Asparamide, L-days Winterine and L-cysteine.

Embodiment 2

[0029]A stem cell medium having high amplification efficiency, composed of MEM medium and adding components including: sodium selenite having a concentration of 30 ng / ml, sodium concentration of 12 ng / ml, Insulin having a concentration of 17 μg / ml, a concentration of 2 μg / ml of a concentration of 20 ng / ml of a fibroblast growth factor, a concentration of 0.5 ng / ml of epidermal growth factor, a concentration of 7 ng / ml vitamin and reduction Antiocooose enzyme III for 1 ng / ml of a concentration of 1 ng / ml, the protein added a concentration of 1.2 g / dL, 2 g / dl albumin, the vitamin and reduction type substance including D-flue-ingolicate L, Folic acid, nicotinamide, pyridoxine, riboflavin, vitamin B35, vitamin B60, vitamin B12, inositol, glycine, L-alanine, L-arginine, L-asparagine, L-day winter Axine and L-cysteine.

Embodiment 3

[0031]A stem cell medium having high amplification efficiency, composed of RPMI-1640 medium and adding components including: selenine acid having a concentration of 60 ng / ml, selenium concentration of 16 ng / ml. Sodium, a concentration of 4 μg / ml of protein, a concentration of 40 ng / ml of an alkaline fibroblast growth factor, a concentration of 1.1 ng / ml epidermal growth factor, a vitamin having a concentration of 7 ng / ml And anti-coupurase III for reduction type substance and concentration of 1.5 ng / ml, the protein added a concentration of 1.5 g / dl of globulin and 3 g / dl albumin, the vitamin and reduction materials including D-pantothenic acid Calcium L, folic acid, nicotinamide, pyridoxine, riboflavin, vitamin B35, vitamin B60, vitamin B12, inositol, glycine, L-alanine, L-arginine, L-Asparamide, L Aspartate and L-cysteine.

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Abstract

The invention is applicable to the technical field of biology, and provides a stem cell culture medium with high amplification efficiency. The stem cell culture medium consists of a basal culture medium and additive components, the additive components comprise genistein, sodium selenite, hormone, protein, basic fibroblast growth factors, epidermal growth factors, vitamins, reducing substances andenzyme inhibitors, the genistein is added into the culture medium, and the genistein interacts and influences with cytokines, so that the genistein and the cytokines jointly play a good role in promoting the growth of stem cells. The culture medium is used for cell culture, has the advantages of short cell cycle, high multiplication capacity, good cell homogeneity and high purity, effectively inhibits differentiation of the stem cells and adherent growth of endothelial cells, in addition, the culture medium does not contain fetal calf serum and other animal-derived components, and the stem cells obtained by the culture medium are suitable for clinical application.

Description

Technical field[0001]The invention belongs to the biological field, and more particularly to a stem cell medium having high amplification efficiency.Background technique[0002]Stem cells are a new research topic that is generally concerned about the 21st century biomedical community. Stem cells are a class of multis-potential cells with self-replication capabilities. Under certain conditions, it can differentiate into a variety of functional cells. Depending on the developmental stage of stem cells is divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, three categories: all-in-range stem cells, multi-capable cells and single-energy stem cells. Stem cells are a non-differentiated, immature cells, with a potential function of regenerating various tissue organs and humans, and the medical community is called "universal cells." Stem cell culture medium is a special culture environment designed during the culture process of stem ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0602C12N2500/30C12N2500/38C12N2501/115C12N2501/11C12N2501/33C12N2501/998C12N2500/35C12N2500/32C12N2501/734C12N2501/999
Inventor 陈晓栋尚倩雯贾善粉张胜超王旻贤马慧
Owner 无锡华泰创新药技术研究院有限公司
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