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Mussel-like coordination adhesive based on epsilon-polylysine and preparation method of mussel-like coordination adhesive

A technology of polylysine and adhesives, applied in surgical adhesives, medical science, surgery, etc., can solve the problems of low immune response, poor safety, easy to cause inflammation, etc., and achieve low cytotoxicity and high adhesive strength Effect

Inactive Publication Date: 2021-03-09
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former has good biocompatibility, biodegradability, and low immune response, but its bonding strength is not high, and it has the risk of being infected by viruses. Although the latter has high bonding strength, its safety is poor and it is easy to Inflammation, and even tumor formation, from this point of view, the existing bioadhesives are difficult to meet the clinical needs

Method used

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  • Mussel-like coordination adhesive based on epsilon-polylysine and preparation method of mussel-like coordination adhesive
  • Mussel-like coordination adhesive based on epsilon-polylysine and preparation method of mussel-like coordination adhesive
  • Mussel-like coordination adhesive based on epsilon-polylysine and preparation method of mussel-like coordination adhesive

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Synthesis of catechol-modified polylysine (PL-Cat): under nitrogen, 1.5g ε-polylysine (PL) was dissolved in 50mL distilled water, 1.28g 3,4 dihydroxyphenylpropane acid (DOHA) was dissolved in 30 mL of distilled water, and the two were mixed evenly, and then 6.74 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl ) and 809mg of N-hydroxysuccinimide (NHS) mixed aqueous solution 30mL, adding 2M hydrochloric acid (HCl) to adjust the pH of the solution to 5.5, mixed reaction for 24h, the product was first treated with PBS (pH5.5 ) dialyzed for 48 hours, then dialyzed against distilled water for 10 hours, and finally freeze-dried for 48 hours to obtain catechol-modified ε-polylysine (PL-Cat), the main component of the adhesive.

[0035] Preparation of mussel-like coordination binder based on ε-polylysine (PL): Dissolve 50 mg PL-Cat in 82.8 μL distilled water, add 22.2 μL FeCl to it 3 Aqueous solution (128mg / mL), vortex to mix evenly, add 5M NaOH to it ...

Embodiment 2

[0037] Synthesis of catechol-modified polylysine (PL-Cat): under nitrogen, 3gε-polylysine (PL) was dissolved in 100mL distilled water, 2.56g 3,4 dihydroxyphenylpropionic acid (DOHA) was dissolved in 60 mL of distilled water, and the two were mixed evenly, and then 13.48 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) was added thereto and 60mL of mixed aqueous solution of 1.618g N-hydroxysuccinimide (NHS), add 5M hydrochloric acid (HCl) to adjust the pH of the solution to 5.5, mix and react for 24h, and the product is first treated with PBS (pH5.5 ) dialyzed for 48 hours, then dialyzed against distilled water for 10 hours, and finally freeze-dried for 48 hours to obtain catechol-modified ε-polylysine (PL-Cat), the main component of the adhesive.

[0038] Preparation of mussel-like coordination binder based on ε-polylysine (PL): Dissolve 100 mg PL-Cat in 152.7 μL distilled water, add 53.2 μL FeCl 3 Aqueous solution (128mg / mL), vortex to mix evenly, a...

Embodiment 3

[0040] Synthesis of catechol-modified polylysine (PL-Cat): under argon atmosphere, 3gε-polylysine (PL) was dissolved in 100mL distilled water, and 5.12g 3,4 dihydroxyphenylpropane Acid (DOHA) was dissolved in 120mL of distilled water, and the two were mixed evenly, then 26.96g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl ) and 3.236g of N-hydroxysuccinimide (NHS) mixed aqueous solution 120mL, adding 5M hydrochloric acid (HCl) to adjust the pH of the solution to 5.5, mixed for 24h, the product was first treated with PBS (pH5. 5) Dialyze for 48 hours, then dialyze against distilled water for 10 hours, and finally freeze-dry for 48 hours to obtain catechol-modified ε-polylysine (PL-Cat), the main component of the adhesive.

[0041]Preparation of mussel-like coordination binder based on ε-polylysine (PL): 100 mg PL-Cat was dissolved in 137.4 μL distilled water, and 66.6 μL FeCl was added to it 3 Aqueous solution (128mg / mL), vortex to mix evenly, add 4M ...

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Abstract

The invention discloses a mussel-like coordination adhesive based on epsilon-polylysine and a preparation method of the mussel-like coordination adhesive. Key factors of a mussel adhesion environmentare introduced into the design of a biological adhesive; and the prepared adhesive has tissue adhesion performance and low cytotoxicity. The preparation method comprises the following steps of: firstly, uniformly mixing epsilon-polylysine with 3,4-dihydroxyphenylpropionic acid under an oxygen-free condition, then adding 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride and N-hydroxysuccinimide into the mixture, and performing mixing and reacting to obtain the main component catechol modified epsilon-polylysine of the adhesive; dissolving PL-Cat in distilled water, adding Fe<3+> intothe distilled water, and adding NaOH to adjust the pH to be weakly alkaline, so that the PL-Cat / Fe adhesive can be obtained. The adhesive has good adhesive strength and low cytotoxicity, and has a wide application prospect in tissue engineering.

Description

technical field [0001] The invention relates to the technical field of preparation of bioadhesives, in particular to a mussel imitation coordination adhesive based on ε-polylysine (PL) and a preparation method thereof. Background technique [0002] Biological (tissue) adhesives are of great significance to the healing of wounds after trauma, and the commonly used adhesives are mainly fibrin-based adhesives and cyanoacrylate-based adhesives. The former has good biocompatibility, biodegradability, and low immune response, but its bonding strength is not high, and it has the risk of being infected by viruses. Although the latter has high bonding strength, its safety is poor and it is easy to Inflammation is caused, and even tumors are generated. From this point of view, the existing bioadhesives are difficult to meet the clinical needs. Therefore, the preparation of novel bioadhesives with good biocompatibility, safety, ease of use, and high bond strength is of great significa...

Claims

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Application Information

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IPC IPC(8): A61L24/04A61L24/00
CPCA61L24/001A61L24/046C08L77/04
Inventor 赵瑾李思迪陈宁原续波侯信
Owner TIANJIN UNIV
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