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Flos lonicerae-herba artemisiae-fructus gardeniae extract and preparation method and application thereof

A technology of gardenia extract and honeysuckle, applied in the field of medicine, can solve problems such as less CV-A6, and achieve the effects of improving pharmacological activity and reducing the dosage of medicines

Inactive Publication Date: 2021-03-09
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are few related studies on CV-A6 at present, and research on anti-CV-A6 drugs is urgently needed

Method used

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  • Flos lonicerae-herba artemisiae-fructus gardeniae extract and preparation method and application thereof
  • Flos lonicerae-herba artemisiae-fructus gardeniae extract and preparation method and application thereof
  • Flos lonicerae-herba artemisiae-fructus gardeniae extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Extraction of a moietic ether site, an ethyl acetate, a n-butanol site

[0037]Crush gold and silver, artemisia, scorpion, over 60 mesh sieve, according to honeysuckle: Artemisia: Scorpion weight ratio 3: 15: 2 ratio is 3: 6.0kg, the coarse powder is 6.0kg, add 8 times The amount of 75% ethanol was soaked for 2 h, and the ultrasound was extracted at room temperature for 20 min, extracted twice. Pumped filtration, combined with filtrate. The extract is 40 ° C to recover ethanol, stirred overnight, to obtain a rough liquid, extract it with petroleum ether, ethyl acetate, n-butanol, to obtain petroleum ether, ethyl acetate, n-butanol site, and water part, take The petroleum ether is part, the ethyl acetate site, the n-butanol site, that is, it is obtained. See the extraction process figure 1 .

[0038] The addition of the volume ratio of 1: 1 in the bude liquid, slightly stirred, shaken, and allows the layering. The lower water is inserted, and the petroleum ether l...

Embodiment 2

[0041] Example 2 Separation of a petroleum ether site

[0042] Separation of purified petroleum ether is separated by silica gel column chromatography figure 2 .

[0043] Example 1 A total of 103 g of the petroleum ether is 103 g, and 3 g, the remaining silica gels are onto the column. Silica gel wet formation column, dry method, and then deodoxicle is covered. Elution with petroleum ether-acetate (10: 0-7: 3, v / v) gradient, the elution volume of each gradient is twice the volume of the column, 25 ml / min, and collects 500 ml per grant. The eluate was carried out by the TLC method, and the thin layer chromatinic silica G board was spotted, and the unfolder was petroleum ether-ethyl acetate, and the developer was 5% vanillal sulfuric acid. The hair dryer is heated, observed the spots, combined with the same flow, and a total of 11 sets of flow.

Embodiment 3

[0044] Example 3 Isolation of EtOAc EtOAc

[0045] Separation process of ethyl acetate image 3 .

[0046] The purified ethyl acetate is separated by silica gel column chromatography. Appropriate amounts of ethyl acetate were taken, respectively, with petroleum ether-ethyl acetate (20:80), dichloromethane-methanol (95: 5), which is better, and therefore, chlorine is selected. Methane-methanol system for elution. A total of 169 g of ethyl acetate is 1 g, and the remaining silica gel samples are left. Silica gel wet formation column, dry method, and then deodoxicle is covered. Elution with dichloromethane-methanol system (10: 0-7: 3, v / v) gradient, each gradient elution volume is 2 times that of the column volume, flow rate 20ml · min -1 Press 500ml of each serving. The eluate was carried out by TLC method, dotted on the thin layer chromatinic silica G plate, expandant dichloromethane-methanol, and the selection agent was 5% vanillic aldehyde silicate and 2% trichloride solution. ...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to a flos lonicerae-herba artemisiae-fructus gardeniae extract, a preparation method thereof and application of the flos lonicerae-herba artemisiae-fructus gardeniae extract to preparation of anti-Coxsackievirus A6 medicines. Antiviral experiment results show that the petroleum ether part, the ethyl acetate part andthe n-butyl alcohol part of the flos lonicerae-herba artemisiae-fructus gardeniae alcohol extract have high anti-CV-A6 activity; Fractions H and J of the petroleum ether part of the flos lonicerae-herba artemisiae-fructus gardeniae alcohol extract have high anti-CV-A6 activity; the fraction E of the ethyl acetate part of the flos lonicerae-herba artemisiae-fructus gardeniae alcohol extract has good anti-CV-A6 activity. and 30% alcohol and 60% alcohol elution products of the n-butyl alcohol part of the flos lonicerae-herba artemisiae-fructus gardeniae alcohol extract have high anti-Coxsackievirus A6 activity.

Description

Technical field [0001] The present invention belongs to the field of medical technology, and specifically involves the use of Honeysuckle - Artemisia - Gardenia extracts and their preparation methods and their application in the preparation of anti-A6-type Coaqi virus drugs. Background technique [0002] Since 2008, the A6 Coveievirus (CV-A6) has begun to popularize worldwide, and has gradually spread from Europe to Asia, and the CV-A6 infection is the rise and foot and low-level disease rate. In 2008, CV-A6 positive hand and foot disease was first lost in Finland. In 2009, China Taiwan, 2010 Spain, 2011 Japan, 2013, my country, Guangdong, Guangdong, China, China, etc., has emerged in the hands and foot of the CV-A6 infection. Sick epidemic. Since 2013, CV-A6 has become one of the main pathogens of the outbreak of hand and foot and mouth disease. Domestic CV-A6 infection causes the risk of hand and foot and mouth disease, and Patients with CV-A6 infection have risen from 8.2% in ...

Claims

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Application Information

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IPC IPC(8): A61K36/744A61P31/14
CPCA61K36/282A61K36/355A61K36/744A61K2236/333A61K2236/39A61K2236/55A61P31/14A61K2300/00
Inventor 李菲田景振
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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