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Chinese wolfberry glycopeptide, preparation method and applications thereof

A wolfberry and glycopeptide technology, applied in the field of medicine, can solve the problems of ethanol explosion protection and solvent recovery

Active Publication Date: 2017-08-08
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alcohol precipitation is a relatively common method to extract polysaccharides, but there are problems of explosion protection and solvent recovery in the production of ethanol, so if there is a new simple and environmentally friendly extraction production method to replace alcohol precipitation operation, it has great application prospects

Method used

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  • Chinese wolfberry glycopeptide, preparation method and applications thereof
  • Chinese wolfberry glycopeptide, preparation method and applications thereof
  • Chinese wolfberry glycopeptide, preparation method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] 100g of dried wolfberry fruit is crushed and soaked in deionized water with a mass ratio of 15 times, the soaking temperature is 10°C, and the soaking time is 10 hours. The soaking solution was placed in a CR22G centrifuge and centrifuged at 1000 rpm for 1 minute, and the supernatant obtained by centrifugation was still turbid. Put the supernatant in a water bath to raise the temperature to 40°C and keep it for 5 hours. The pulp and pectin remaining in the supernatant polymerize into a flocculent precipitate, and then put the material in a CR22G centrifuge at a speed of 16000rpm for 5 hours. Seconds, a clear solution was obtained with a light transmittance of 83%. Gained clarified solution is placed in the ultrafiltration device for ultrafiltration, the molecular weight cut-off of the ultrafiltration membrane is 1000Da, and the working pressure is 5 kilograms, constantly replenishes deionized water in the intercepted solution, when the conductivity of the retained solut...

Embodiment 2

[0052]100g of dried wolfberry fruit is crushed and soaked in deionized water with a mass ratio of 10 times, the soaking temperature is 20°C, and the soaking time is 5 hours. The soaking solution was placed in a CR22G centrifuge and centrifuged at 4000 rpm for 10 seconds, and the supernatant obtained by centrifugation was still turbid. Put the supernatant in a water bath to raise the temperature to 60°C and keep it for 2 hours. The pulp and pectin remaining in the supernatant polymerize into a flocculent precipitate. Then put the material in a CR22G centrifuge and centrifuge at 13000rpm for 5 minutes. A clear solution was obtained with a light transmittance of 78%. Gained clarified solution is placed in the ultrafiltration device for ultrafiltration, the molecular weight cut-off of the ultrafiltration membrane is 1000Da, and the working pressure is 5 kg, and deionized water is constantly added to the intercepted solution. When the conductivity of the retained solution drops to ...

Embodiment 3

[0054] 100g of dried wolfberry fruit is crushed and soaked in deionized water with a mass ratio of 5 times, the soaking temperature is 30°C, and the soaking time is 2 hours. The soaking solution was placed in a CR22G centrifuge and centrifuged at 3000 rpm for 1 minute, and the supernatant obtained by centrifugation was still turbid. Put the supernatant in a water bath to raise the temperature to 70°C and keep it for 0.5 hours. The pulp and pectin remaining in the supernatant will polymerize into a flocculent precipitate. Then put the material in a CR22G centrifuge and separate it at 6000rpm for 5 minutes , a clear solution was obtained with a light transmittance of 60%. The resulting clarified solution is placed in an ultrafiltration device for ultrafiltration. The molecular weight cut-off of the ultrafiltration membrane is 2000Da, and the working pressure is 5 kg. Deionized water is constantly added to the retained solution. When the conductivity of the retained solution drop...

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Abstract

The present invention discloses a Chinese wolfberry glycopeptide, a preparation method and applications thereof, wherein the molecular weight distribution of the Chinese wolfberry glycopeptide is that the Chinese wolfberry glycopeptide having the molecular weight of 1000-10000 Da accounts for 50-85%, the protein content is 20-35%, the neutral polysaccharide content is 20-35%, and the uronic acid content is 5-20%. According to the present invention, the heating flocculation method is used to remove the partial insoluble impurities in the preparation method of the present invention and replaces the application of a large amount of ethanol to precipitate in the traditional extraction method, such that the production safety is improved, the ethanol recovery step is avoided, the production cost is reduced, the activity of the obtained Chinese wolfberry glycopeptide is not changed, and the god production promotion prospect is provided.

Description

technical field [0001] The present invention relates to the field of medical science and technology, in particular to a wolfberry glycopeptide and its preparation method and its application in the preparation of immune-enhancing drugs or health care products. Background technique [0002] Lycium barbarum is the mature fruit of Lycium barbarum, a plant of the genus Lycium barbarum in the Solanaceae family. It has a variety of health benefits and is a dual-purpose food for medicine and food approved by the Ministry of Health. [0003] The main active ingredient of wolfberry is wolfberry glycopeptide. It is a water-soluble glycoprotein. Many studies have shown that Lycium barbarum glycopeptide has the functions of promoting immunity, anti-aging, anti-tumor, scavenging free radicals, anti-fatigue, anti-radiation, protecting liver, protecting and improving reproductive functions, etc. Traditional wolfberry glycopeptide extraction methods mostly use high-temperature cooking, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30A23L33/18A61K38/02A61P37/04
CPCA23V2002/00A61K38/02C07K1/30C07K1/34C07K1/36A23V2200/324A23V2250/55
Inventor 吕龙江艳项玉华丁志刚韩建林智通卫
Owner SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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