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Establishment of a highly sensitive detection method for dengue virus based on nano-magnetic beads immunochromatography

A technology of nano-magnetic beads and dengue virus, which is applied in measurement devices, analytical materials, biological material analysis, etc., can solve the problems of cumbersome virus separation operation, unfavorable large-scale detection, unsuitable early diagnosis, etc. The effect of simplicity and improved sensitivity

Active Publication Date: 2022-08-02
CHINESE ACAD OF INSPECTION & QUARANTINE
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Problems solved by technology

Among them, the virus isolation operation is cumbersome and has low sensitivity, which is not suitable for early diagnosis; the RT-PCR method requires professional equipment, the process is complicated, the cost is high, and it is not conducive to large-scale detection; the ELISA method is complicated to operate, and its sensitivity and specificity are lower than RT. -PCR method; colloidal gold method is slightly lower in sensitivity and specificity than ELISA method, and the cost is higher than ELISA method

Method used

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  • Establishment of a highly sensitive detection method for dengue virus based on nano-magnetic beads immunochromatography
  • Establishment of a highly sensitive detection method for dengue virus based on nano-magnetic beads immunochromatography
  • Establishment of a highly sensitive detection method for dengue virus based on nano-magnetic beads immunochromatography

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Experimental program
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Embodiment 1

[0073] 1 Materials, methods and technical routes

[0074] 1.1 Virus

[0075] Dengue virus recombinant protein NS1 (E.coli) used in this study, Zika virus (ZIKV), influenza virus and serum samples were provided by Beijing Dahong Biotechnology Co., Ltd.

[0076] 1.2 Materials and Instruments

[0077] Sodium acetate, FeCl 3 ·6H 2 O, ethanol, ethylene glycol (Beijing Chemical Reagent Factory), 10mg / mL N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide. Hydrochloride (EDC), 10mg / mL N-Hydroxythiosuccinimide. Sodium salt (NHS), 0.1MTris, 5%BSA, 1%BSA, 10mM PB, DAB color developing solution (Zhongshan Jinqiao), magnetic beads (Jinzhun Zhizao Biotechnology Co., Ltd., particle size 50nm), transmission electron microscope (JEOL2000FX 20\0KV, Japan Electronics Co., Ltd.), high temperature oven (Tianjin Test), enzyme-linked immunosorbent analyzer (Bio-Rad), PVDF membrane (Millipore), scribing flow machine (Arista, USA), slitting machine (Tianjin Keju Biotechnology), XYZ three-dimensional ...

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Abstract

The invention discloses the establishment of a high-sensitivity detection method for dengue virus based on nano-magnetic bead immunochromatography technology, the preparation of dengue virus nano-magnetic bead-antibody probes; the assembly of nano-magnetic bead test strips; the use of nano-magnetic bead test strips to detect For the detection of dengue virus, a new method for the detection of dengue virus based on the immunochromatographic technology of nano-magnetic beads is established by using the magnetic enrichment and separation function of nano-magnetic beads and the characteristic that the color signal can be observed by the naked eye, which can effectively improve the detection sensitivity. The method is simple and easy to operate, and can be used for large-scale rapid diagnosis.

Description

technical field [0001] The invention relates to the technical field of nano-magnetic beads immunochromatography. Specifically, a highly sensitive detection method for dengue virus was established based on nano-magnetic beads immunochromatography technology. Background technique [0002] Dengue fever (Dengue Fever) is an acute infectious disease caused by Dengue Virus (DENV) of the genus Flavivirus. Dengue virus is transmitted by vector insects such as Aedes aegypti and Aedes albopictus and causes dengue fever. It is prevalent in tropical and subtropical regions of the world. Mild symptoms include persistent fever, headache, joint pain, muscle pain, etc., and severe disease has a high mortality rate. High dengue hemorrhagic fever and dengue shock syndrome. The rather fast-spreading viral disease has seen a 30-fold increase in global incidence over the past 50 years due to factors such as global warming and blind urban sprawl. According to statistics, 390 million people wor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/558G01N33/54346G01N33/54326G01N2333/185
Inventor 刘键刘昕张永江
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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