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Liquid chromatography-tandem mass spectrometry method for determining residual quantity of ametoctradin in vegetable food

A technology of fenflux and tandem mass spectrometry, which is applied in the field of liquid chromatography tandem mass spectrometry for determination of fenflux residues in plant foods, and can solve the problems of long time, cumbersome extraction steps, false positive results and the like.

Pending Publication Date: 2021-03-05
PONY TESTING INT GRP SHANGHAI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of liquid chromatography ultraviolet detector for detection is prone to false positive results
The traditional solid-phase extraction column, the extraction steps are cumbersome and time-consuming

Method used

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  • Liquid chromatography-tandem mass spectrometry method for determining residual quantity of ametoctradin in vegetable food
  • Liquid chromatography-tandem mass spectrometry method for determining residual quantity of ametoctradin in vegetable food
  • Liquid chromatography-tandem mass spectrometry method for determining residual quantity of ametoctradin in vegetable food

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Experimental program
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Effect test

Embodiment 1

[0037] 1. Instruments and reagents

[0038] High performance liquid chromatography-tandem mass spectrometer: American Waters, UPLC-Xevo TQ;

[0039] Standard substance: pyraclostrobin, 100mg / L;

[0040] Methanol: chromatographically pure;

[0041] Acetonitrile: chromatographically pure;

[0042] Formic acid: analytically pure

[0043] Anhydrous magnesium sulfate: analytically pure;

[0044] Oasis PRiME HLB: 6mL, 200mg;

[0045] The water used in this method is primary water.

[0046] 2. Instrument analysis conditions

[0047] a. Liquid chromatography conditions:

[0048] Chromatographic column: Waters, BEH C18, 1.7μm, 2.1mm×50mm

[0049] Mobile phase: 0.1% formic acid aqueous solution (A) + 0.1% formic acid acetonitrile solution (B), gradient elution, the program is the initial mobile phase ratio, 0.1% formic acid aqueous solution; 0.1% formic acid acetonitrile solution 95:5; 2min, 0.1% Formic acid aqueous solution: 0.1% formic acid acetonitrile solution 95:5; 5.5min,...

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Abstract

The invention belongs to the technical field of food safety, and relates to a liquid chromatography-tandem mass spectrometry method for measuring residual quantity of ametoctradin in vegetable food. The method comprises the steps of weighing a uniform sample, carrying out homogeneous extraction by using methanol, purifying by using anhydrous magnesium sulfate and PSA, carrying out high-speed refrigerated centrifugation, carrying out rotary evaporation by using a supernatant until the sample is nearly dry, dissolving residues by using 80% acetonitrile, purifying by using an Oasis PRiME HLB column, filtering by using a 0.22 [mu] m filter membrane, carrying out liquid chromatography-tandem mass spectrometry detection, and quantifying by using an external standard method. The detection methodprovided by the invention has the advantages of simple and novel pretreatment steps, good impurity removal effect, high sensitivity, high recovery rate and good precision of determination results, andcan effectively detect the residual quantity of ametoctradin in vegetable food.

Description

technical field [0001] The invention belongs to the technical field of food safety, and in particular relates to a method for determining the residual amount of pyramethyzin in vegetable foods by liquid chromatography tandem mass spectrometry. [0002] technical background [0003] Pyramid is a triazole-pyrimidine fungicide, which belongs to the mitochondrial respiration inhibitor. It is a highly selective fungicide, which has a control effect on downy mildew and Phytophthora oomycete fungi, and has a strong residual activity And rain resistance, can redistribute in the leaves, protect the healthy growth of crops, and give full play to their growth potential. At the beginning of 2010, pyraclostrobin was firstly registered in Romania, mainly for the control of late blight and downy mildew on grapes; in July 2010, it was approved to be registered in the UK; in the same year, it was registered in the Netherlands; The amine product Initium was launched; in 2012, it was registered...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 熊敏
Owner PONY TESTING INT GRP SHANGHAI CO LTD
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