Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Long-chain non-coding RNA RP11-469H8.6 and application thereof

A long-chain non-coding, tumor technology, applied in the field of long-chain non-coding RNA RP11-469H8.6, which can solve the problems of low expression and function without any literature reports

Active Publication Date: 2021-03-05
CHINA PHARM UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Through bioinformatics analysis, the present invention found for the first time that lncRNA RP11-469H8.6 was significantly lower expressed in various solid tumors including head and neck cancer, thyroid cancer, lung cancer, esophageal squamous cell carcinoma, gastric cancer, breast cancer, kidney cancer, and skin cancer , through database and literature search (Pubmed, Ensembl, Google), it is found that the function of RP11-469H8.6 has not been reported in any literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Long-chain non-coding RNA RP11-469H8.6 and application thereof
  • Long-chain non-coding RNA RP11-469H8.6 and application thereof
  • Long-chain non-coding RNA RP11-469H8.6 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Expression analysis of RP11-469H8.6 in various human tumor tissues and paracancerous tissues.

[0041] TCGA standard method download includes head and neck cancer, glioma, thyroid cancer, esophageal squamous cell carcinoma, lung cancer, liver cancer, gastric cancer, kidney cancer, breast cancer, ovarian cancer, cervical cancer, bladder cancer, colorectal cancer, pancreatic cancer, osteosarcoma The RNA-seq sequencing files and clinical information of cancer tissues and normal tissues of 16 kinds of tumors, including skin cancer, found the differential expression of RP11-469H8.6 in Table 1 (judgment criteria: (1)|cancer / paracancer The expression level of |>2, (2)P<0.05).

[0042] Table 1 Analysis of the expression level of RP11-469H8.6 in human tumor tissues and normal tissues (cancer / paracancer)

[0043]

[0044]

[0045] From Table 1 and figure 1 As shown, compared with normal tissues, the expression levels of RP11-469H8.6 were significantly higher in 8 tumor ti...

Embodiment 2

[0047] The expression of RP11-469H8.6 in head and neck cancer clinical patients and adjacent tissues was detected by fluorescent quantitative PCR.

[0048] (1) Collection of specimens

[0049] With the informed consent of the patients, head and neck cancer and paracancerous tissue samples were collected during the operation, washed with normal saline, and stored in liquid nitrogen or in a -80°C refrigerator for later use.

[0050] (2) Primer design

[0051]Primers were designed using Primer Premier 5.0 according to the nucleotide sequence of lnc RP11-469H8.6, the sequence is as follows:

[0052] Upstream primer (SEQ ID NO.2)

[0053] Downstream primer (SEQ ID NO.3)

[0054] (3) Real-time quantitative PCR detection of the expression of RP11-469H8.6 in head and neck cancer patients and normal paracancerous tissues.

[0055] The total RNA of the collected samples was extracted according to the instructions of Trizol of the life company, and then the purity and concentration o...

Embodiment 3

[0063] In situ hybridization was used to detect the expression of RP11-469H8.6 in head and neck cancer clinical patients and adjacent tissues.

[0064] (1) Collection and processing of tissue samples

[0065] With the informed consent of the patients, head and neck cancer and paracancerous tissue samples were collected during the operation, washed with normal saline, and fixed in formalin fixative for at least 4 hours. Paraffin-embedded and sectioned, routinely deparaffinized to water.

[0066] (2) Digoxigenin-labeled probes synthesized in vitro

[0067] Design a specific probe according to the nucleotide sequence of RP11-469H8.6, the specific sequence is shown in SEQ ID NO.4, and label several DIG-11-dUTP molecules at both ends of the probe by covalent bonding.

[0068] (3) Hybridization and immunoassay

[0069] Add 50-100ul of hybridization solution to each slide, cover the slide with a 22X22 coverslip, seal the slide with glue (to prevent drying), incubate at a hybridiza...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a long-chain non-coding RNA RP11-469H8.6 and application thereof, and belongs to the field of tumor detection and molecular targeted therapy. Experiments prove that the long-chain non-coding RNA RP11-469H8.6 is remarkably low in expression in various solid tumors such as head and neck cancer, thyroid cancer, lung cancer, esophageal squamous carcinoma, gastric cancer, breastcancer, kidney cancer and skin cancer, and experiments prove that after the lnc RP11-469H8.6 is over-expressed, proliferation and migration invasion of various solid tumors such as head and neck cancer, thyroid cancer, lung cancer, esophageal squamous carcinoma, gastric cancer, breast cancer, kidney cancer and skin cancer of human bodies can be remarkably inhibited, and the long-chain non-codingRNA RP11-469H8.6 has important tumor detection and treatment value.

Description

technical field [0001] The invention belongs to the field of tumor detection and molecular targeted therapy, and more specifically relates to a long-chain non-coding RNA RP11-469H8.6 and its application. Background technique [0002] Tumor is the most serious disease that endangers human health. Studies have found that the occurrence of tumors is a complex process of gradual accumulation of gene mutations. The development of modern medical technology and molecular biology has brought tumor treatment into an era of individualization and greatly increased the remission rate of tumor treatment. Therefore, finding specific targets for early detection, treatment and prognosis of tumors is the key bottleneck restricting the clinical efficacy of tumors. [0003] Long non-coding RNA (long non-coding RNA, lncRNAs) is a class of RNA molecules with a transcript length of more than 200 nt, which lacks a specific and complete open reading frame and has no protein-coding function. quite...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K31/7105A61P35/00C12N15/11
CPCC12Q1/6886A61K31/7105A61P35/00C12Q2600/178C12Q2600/118
Inventor 徐寒梅李梦玮
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com