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Electrochemical immunosensor for simultaneously detecting two neuroendocrine tumor markers

A tumor marker and neuroendocrine technology, applied in the field of electrochemical biosensing, can solve the problem that there is no simultaneous detection of biosensors, and achieve the effect of enhancing antifouling ability and improving specificity

Pending Publication Date: 2021-02-09
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the realization of simultaneous detection of serum CgA and CgB is of great significance for improving the accuracy of clinical diagnosis of NETs, ​​but there is no biosensor for simultaneous detection of CgA and CgB

Method used

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  • Electrochemical immunosensor for simultaneously detecting two neuroendocrine tumor markers
  • Electrochemical immunosensor for simultaneously detecting two neuroendocrine tumor markers
  • Electrochemical immunosensor for simultaneously detecting two neuroendocrine tumor markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Preparation of an electrochemical immunosensor for simultaneous detection of CgA and CgB

[0040] (1) PMS-M 2+ preparation of

[0041] Add 10mg of PMS to 20mL of copper nitrate or lead nitrate solution, wherein the mass ratio of PMS to copper nitrate (or lead nitrate) is 1:2, ultrasonically disperse the solution and stir at 22°C for 8h, then centrifuge and wash, place at 60 ℃ drying in an oven to obtain PMS-Cu 2+ or PMS-Pb 2+ . figure 2 For the prepared PMS-Cu 2+ scanning electron microscope image of image 3 For the prepared PMS-Pb 2+ scanning electron microscope image.

[0042] (2) PMS-M 2+ Preparation of / AuNPs / PEG / CS

[0043] Solution A: the concentration is 2mgmL -1 PMS-Cu 2+ or PMS-Pb 2+ Solution; Solution B: a mixed solution containing 0.5wt.% PEG, 0.5wt.% CS and 0.5mM AuNPs. Mix solutions A and B at a volume ratio of 1:1 and disperse them ultrasonically.

[0044] (3) Preparation of sensor working electrode

[0045] 1) Add 6μL PMS-Cu 2+ / AuNPs...

Embodiment 2

[0063] 1. Preparation of an electrochemical immunosensor for simultaneous detection of CgA and CgB

[0064] (1) PMS-M 2+ preparation of

[0065] Add 8 mg of PMS to 10 mL of copper nitrate or lead nitrate solution, wherein the mass ratio of PMS to copper nitrate (or lead nitrate) is 1:0.5, ultrasonically disperse the solution and stir at 20°C for 6 hours, then centrifuge and wash, and place at 55 ℃ drying in an oven to obtain PMS-Cu 2+ or PMS-Pb 2+ .

[0066] (2) PMS-M 2+ Preparation of / AuNPs / PEG / CS

[0067] Solution A: the concentration is 1mgmL -1 PMS-Cu 2+ or PMS-Pb 2+ Solution; Solution B: a mixed solution containing 0.4wt.% PEG, 0.4wt.% CS and 0.4mM AuNPs. Mix solutions A and B according to the volume ratio of 1:0.5, and ultrasonically disperse.

[0068] (3) Preparation of sensor working electrode

[0069] 1) Add 5μL PMS-Cu 2+ / AuNPs / PEG / CS and PMS-Pb 2+ / AuNPs / PEG / CS solution was dropped on the surface of two clean glassy carbon electrodes respectively, and ...

Embodiment 3

[0078] 1. Preparation of an electrochemical immunosensor for simultaneous detection of CgA and CgB

[0079] (1) PMS-M 2+ preparation of

[0080] Add 12 mg of PMS to 30 mL of copper nitrate or lead nitrate solution, wherein the mass ratio of PMS to copper nitrate (or lead nitrate) is 1:3, ultrasonically disperse the solution and stir at 25 ° C for 10 h, then centrifuge and wash, and place at 65 ℃ drying in an oven to obtain PMS-Cu 2+ or PMS-Pb 2+ .

[0081] (2) PMS-M 2+ Preparation of / AuNPs / PEG / CS

[0082] Solution A: the concentration is 3mgmL -1 PMS-Cu 2+ or PMS-Pb 2+ Solution; Solution B: a mixed solution containing 0.6 wt.% PEG, 0.6 wt.% CS and 0.6 mM AuNPs. Mix solutions A and B at a volume ratio of 1:2 and disperse them ultrasonically.

[0083] (3) Preparation of sensor working electrode

[0084] 1) Add 10μL PMS-Cu 2+ / AuNPs / PEG / CS and PMS-Pb 2+ / AuNPs / PEG / CS solution was dropped on the surface of two clean glassy carbon electrodes respectively, and dried at...

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Abstract

The invention discloses an electrochemical immunosensor for simultaneously detecting two neuroendocrine tumor markers and belongs to the technical field of electrochemical biosensing. According to thesensor, two parallel glassy carbon electrodes are used as working electrodes, and the surfaces of the electrodes are sequentially modified with a metal ion functionalized porous magnesium silicate / gold nanoparticle / polyethylene glycol / chitosan composite material and a recognition antibody. When specific binding occurs between the markers and the antibody, differentiable electric signals of the two metal ions are recorded through differential pulse voltammetry to realize simultaneous detection of CgA and CgB, and the more the markers react with the antibody, the lower the electric signals are.The electrochemical immunosensor is advantaged in that the electrochemical immunosensor is high in sensitivity and low in detection limit, the limitation of a single marker can be made up, and accuracy of neuroendocrine tumor diagnosis is improved.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensing, and provides a method for simultaneously detecting neuroendocrine tumor markers, that is, simultaneously detecting two neuroendocrine tumor (NETs) markers, chromogranin A (CgA) and chromogranin B (CgB) electrochemical immunosensor. Background technique [0002] Neuroendocrine tumors (NETs) are a group of rare tumors of neuroendocrine cell origin. In recent years, the incidence of NETs has continued to increase worldwide. NETs are usually asymptomatic in the early stage, and 60–80% of NETs patients are diagnosed at advanced stage, and NETs such as rectum and pancreas are easily misdiagnosed and mistreated clinically. Therefore, effective tumor marker screening and early diagnosis of NETs are crucial to improve patient treatment outcomes. [0003] Currently, serum chromogranin A (CgA) is the most commonly used marker in NETs, ​​which can be used to assist diagnosis, guide trea...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48G01N33/574
CPCG01N27/308G01N27/3275G01N27/48G01N33/57407G01N33/57488
Inventor 陈旭刘雪娇谭煌英李远良冯拥军
Owner BEIJING UNIV OF CHEM TECH
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