Bioreactor auxiliary device and operation method thereof

Abstract

The invention discloses a bioreactor auxiliary device and an operation method thereof. The bioreactor auxiliary device comprises bioreactors, a sampler, a storage and analysis device, a buffer solution bottle, a waste solution bottle and feedback supplementing pumps, wherein the bioreactors are connected with the sampler, the sampler is connected with the storage and analysis device, the buffer solution bottle and the waste solution bottle are connected between the sampler and the storage and analysis device, and the feedback supplementing pumps are connected to the bioreactors through a silica gel hose. The operation method of the bioreactor auxiliary device comprises automatic sampling operation, automatic sample storage operation, automatic analysis operation, automatic cleaning operation and automatic feedback material supplementing operation. According to the invention, operations such as full-automatic sampling, sample storage, analysis, cleaning, feedback supplementing and the like can be realized. The automation degree is high, time and labor are saved, and data analysis and regulation are accurate.

Description

technical field [0001] The invention relates to an auxiliary device for biological fermentation and cultivation and an operating method thereof, in particular to an auxiliary device for a bioreactor and an operating method thereof. Background technique [0002] In the process of biological fermentation and cultivation, operations such as online sampling of fermentation broth, offline analysis, and feeding regulation are often required. The on-line sampling of fermentation broth is usually done through the sampling valve installed on the reactor. The existing fermentation broth sampling valve generally includes a fermentation broth outlet and a manual valve body connected to the inner wall of the sampling tube. When not sampling, the manual valve body is blocked. When sampling, the operator moves the manual valve body away from the outlet of the fermentation broth, and under the action of the pressure of the tank, the fermentation broth flows out from the outlet of the fermen...

AI Technical Summary

Problems solved by technology

Another reason is that the cycle of most biological fermentations is relatively long, as short as 1-2 days, and as long as more than 15 days. During the fermentation process, continuous sampling and analysis are required to detect the fermentation state or to regulate the fermentation process control strategy. Manual sampling and off-line analysis are time-consuming and labor-intensive in the operation of feedback regulation of the fermentation process, the control accuracy of the fermentation process is not high, and it will indirectly affect the accuracy of the analysis and measurement data

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