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Application of cerasus humilis extract in preparation of antibacterial products

A technology for extracts and products, which is applied in the field of application of Plum extract in the preparation of antibacterial products, can solve the problems of low development and utilization and low added value, and achieve the effect of full utilization

Active Publication Date: 2021-02-05
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development and utilization of Plum pulp is relatively low, and it is mostly used as fruit juice, wine making, preserved fruit, etc., with low added value. Therefore, it is necessary to deeply develop Plum pulp and increase added value in order to realize full utilization of resources.

Method used

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  • Application of cerasus humilis extract in preparation of antibacterial products
  • Application of cerasus humilis extract in preparation of antibacterial products
  • Application of cerasus humilis extract in preparation of antibacterial products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of the total extract of embodiment 1 eucalyptus

[0034] Remove the pits of Ou Li, take the pulp, weigh 330g and put it into a round-bottomed flask, add 2600mL of distilled water, stir well, heat and reflux at room temperature for extraction for 1h (extraction temperature is 100°C), filter with 8 layers of gauze, repeat three times to combine and take The filtrate is then concentrated under reduced pressure (the reduced pressure conditions are water bath temperature 60° C., vacuum degree 0.08 MPa, rotation speed 50 r / min), concentrated until viscous and poured into an evaporating dish. Dry in an electric constant temperature blast drying oven (DHG-9140A) (drying condition temperature: 60°C) until the extract is hard and there is no obvious depression when pressed; weigh 60g of the extract, which is the water extract of Oxyrifolia.

Embodiment 2

[0035] The preparation of embodiment 2 Plum water layer extract, Plum organic solvent layer extract

[0036] According to the method of Example 1, prepare the total extract of Prunus punctatus, weigh 25g and put it into a beaker, add 100mL of distilled water, stir well at room temperature until dissolved, then pour it into a 250mL separatory funnel and add 100mL of dichloromethane, shake fully, Stand still for 24 hours to separate the liquid (the upper layer solution is the water layer, and the lower layer solution is the methylene chloride layer), the lower layer solution is discharged from the lower port, and placed in a 500mL beaker. Then add 100mL of dichloromethane into the separatory funnel, fully shake and stand for 24h to separate the liquid, and the lower layer solution is released from the lower port into a 500mL beaker. This was repeated until the dichloromethane layer was colorless.

[0037] Add 100 mL of ethyl acetate to the water layer extracted by dichlorometha...

Embodiment 3

[0040] The bacteriostasis of embodiment 3 Ou Li extract

[0041] 1. Standard strains: Staphylococcus aureus (26112), Staphylococcus epidermidis (26069), Klebsiella pneumoniae (46117), Shigella flexneri (51571), Proteus (49102), Salmonella typhi (50071) , Pseudomonas aeruginosa (10102), Escherichia coli (44155), the above strains come from the China Institute of Pharmaceutical and Biological Products.

[0042] Test drug sample: the total extract of Prunus punctatus prepared in Example 1, the aqueous layer extract of Prunus punctifera prepared in Example 2, the dichloromethane layer extract of Prunus punctifera, the n-butanol layer extract of Prunus punctifera, the ethyl acetate of Prunus punctatus Ester layer extract.

[0043] Medium:

[0044] Double-material nutrient broth: peptone 20g / L, NaCl 10g / L, beef extract 6g / L, dissolved in distilled water;

[0045] Single-material nutrient broth: peptone 10g / L, NaCl 5g / L, beef extract 3g / L, dissolved in distilled water;

[0046] 2...

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Abstract

The invention discloses application of a cerasus humilis extract in preparation of antibacterial products. The cerasus humilis extract has a remarkable inhibition effect on staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, shigella flexneri, proteus, salmonella typhimurium, pseudomonas aeruginosa and escherichia coli, and has great potential in development and research of antibacterial drugs and other antibacterial products in the future.

Description

technical field [0001] The invention relates to the technical field of phytochemistry, and more specifically relates to the application of Prunus chinensis extract in the preparation of antibacterial products. Background technique [0002] In recent years, due to the abuse of antibiotics, the emergence of drug-resistant strains has become the most difficult problem in clinical treatment. It takes a long period to develop new antibiotics, so it is particularly important to discover traditional Chinese medicines with antibacterial activity or their active components. [0003] Cerasus humilis (Bge.) Sok is a small shrub of the genus Cherry in the family Rosaceae. It is an ancient wild fruit tree species unique to my country. It is widely distributed in arid and cold regions such as North China, Northwest China, Northeast China and East China. Plum fruit is rich in nutrients, among which minerals (Ca, Fe), vitamins (VC, VB family, etc.), polyphenols, amino acids and unsaturated...

Claims

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Application Information

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IPC IPC(8): A61K36/736A01N65/34A01P1/00A61P31/04A61K131/00
CPCA61K36/736A61P31/04A01N65/34A01N65/00A61K2236/331A61K2236/39A61K2236/53A61K2236/51Y02A50/30
Inventor 付雪艳龙俊典陶梦昕王占军刘乾松董琳李航
Owner NINGXIA MEDICAL UNIV
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