Method for preparing GABA (gamma-aminobutyric acid) tea through microbial fermentation
A technology of microbial fermentation and lactic acid bacteria fermentation, applied in the field of microbial fermentation to produce GABA tea, can solve the problems of not being popularized and applied, damage to tea quality, difficult operating conditions, etc., to achieve long-term preservation and storage, increase utilization rate, and uniform quality Effect
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Embodiment 1
[0052] A method for making GABA tea by microbial fermentation, comprising the steps of:
[0053] (1) Preparation of lactic acid bacteria fermented seed liquid: culture Lactobacillus brevis AS1.214 for 12 hours, collect the bacteria after centrifugation, and prepare lactic acid bacteria fermented seed liquid with sterile water for later use.
[0054] (2) Picking fresh leaves: picking fresh tea leaves with one bud and three or four leaves;
[0055] (3) Finishing: use a microwave oven to finish for 2 minutes;
[0056] (4) kneading: adopt kneading machine to knead the green leaves cooled to 30~35°C for 20min;
[0057] (5) Sterilization: Sterilize the rolled fresh leaves in a high-pressure steam sterilizer at 121° C. for 15 minutes.
[0058] (6) Inoculation: soak the sterilized tealeaves in sterile water at a mass ratio of 20%, add the lactic acid bacteria fermented seed liquid obtained in step (1), and inoculum with 3%.
[0059] (7) Fermentation: the above-treated tea leaves we...
Embodiment 2
[0063] A method for making GABA tea by microbial fermentation, comprising the steps of:
[0064] (1) Preparation of lactic acid bacteria fermented seed liquid: culture Streptococcus thermophiles AS1.6472 for 14 hours, collect the bacteria after centrifugation, and prepare lactic acid bacteria fermented seed liquid with sterile water for later use.
[0065] (2) Picking fresh leaves: picking fresh tea leaves with one bud and three or four leaves;
[0066] (3) Finishing: Use a microwave oven to finish for 2.5 minutes;
[0067] (4) Kneading: adopt kneading machine to knead the green leaves cooled to 30~35°C for 15min;
[0068] (5) Sterilization: Sterilize the rolled fresh leaves in a high-pressure steam sterilizer at 121° C. for 15 minutes.
[0069] (6) Inoculation: soak the sterilized tea leaves in sterile water at a mass ratio of 30%, add the lactic acid bacteria fermented seed liquid obtained in step (1), and inoculate with 1%.
[0070] (7) Fermentation: the above-treated te...
Embodiment 3
[0074] A method for making GABA tea by microbial fermentation, comprising the steps of:
[0075] (1) Preparation of lactic acid bacteria fermented seed liquid: Lactobacillus plantarum subsp. plantarum AS1.3919 was cultured for 16 hours, the bacteria were collected after centrifugation, and lactic acid bacteria fermented seed liquid was prepared with sterile water for later use.
[0076] (2) Picking fresh leaves: picking fresh tea leaves with one bud and three or four leaves;
[0077] (3) Finishing: Use a microwave oven to finish for 3 minutes;
[0078] (4) kneading: adopt kneading machine to knead the green leaves cooled to 30~35°C for 20min;
[0079] (5) Sterilization: Sterilize the rolled fresh leaves in a high-pressure steam sterilizer at 121° C. for 15 minutes.
[0080] (6) Inoculation: soak the sterilized tealeaves in sterile water at a mass ratio of 20%, add the lactic acid bacteria fermented seed liquid obtained in step (1), and inoculate with 6%.
[0081] (7) Fermen...
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