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A kind of yellow fluorescent carbon dot and its preparation method and application

A yellow fluorescence, carbon dot technology, used in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of weak emission, limited application of carbon dots, biological tissue damage, etc., and achieve stable optical properties and good detection results. , good solubility and dispersibility

Active Publication Date: 2022-03-18
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the carbon dots exhibit blue-green emission, with weak emission in the long-wavelength region (yellow to red)
The application of carbon dots in the field of biosensing is limited due to the blue autofluorescence of biological matrices and the susceptibility of biological tissues to damage by ultraviolet excitation light.

Method used

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  • A kind of yellow fluorescent carbon dot and its preparation method and application
  • A kind of yellow fluorescent carbon dot and its preparation method and application
  • A kind of yellow fluorescent carbon dot and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1, at room temperature, dissolve 190.1 mg of EGTA and 108.1 mg of p-phenylenediamine in 10 mL of deionized water, stir well, and sonicate to obtain a clear solution.

[0033] Step 2, transfer the solution to a 25mL hydrothermal reaction kettle.

[0034] Step 3: Put the hydrothermal kettle in an oven and react at 200° C. for 5 hours to obtain a brown solution.

[0035] Step 4, take out the hydrothermal reaction kettle, cool naturally, and centrifuge the obtained carbon dot solution at 10000rpm for 10min to remove insoluble matter. Further filter with a 0.22 μm microporous membrane.

[0036] In step 5, the above-mentioned fluorescent carbon quantum dot aqueous solution is freeze-dried to obtain yellow fluorescent carbon dots.

Embodiment 2

[0038] The carbon dot prepared in embodiment 1 is carried out TEM and infrared spectroscopic characterization (see Figure 1-2 ), for UV absorption spectroscopy and fluorescence excitation emission characterization (see image 3 ), the particle size of carbon dots prepared by the present invention is all less than 10nm, and the surface contains groups such as carboxyl, hydroxyl, amino groups, and emits yellow fluorescence.

Embodiment 3

[0040] Get 1mL of the carbon dot aqueous solution (0.30mg / mL) prepared in Example 1 and place it in a fluorescent cuvette, add 10 μL concentration of chlortetracycline solution of 1, 5, 10, 15 ... 95, 100 μM respectively, mix well, Scan the emission spectrum (λex=400nm, λem=540nm) in the fluorescence photometer, according to the relationship between the concentration of aureomycin and the relative fluorescence intensity change value, calculate the detection range and detection limit of carbon dots to aureomycin ( See Figure 4 ).

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Abstract

The invention discloses a carbon dot emitting yellow fluorescence, a preparation method and application thereof. The carbon dot is a yellow fluorescent carbon dot prepared by a one-step hydrothermal method using ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EGTA) and p-phenylenediamine as raw materials. The preparation method of the carbon dot is simple and convenient, and the prepared carbon dot has extremely small size, good water solubility and excellent light stability. Due to the unique surface structure of carbon dots, it can be used as a fluorescent probe to detect tetracycline antibiotics in a label-free, rapid and sensitive manner. When tetracycline antibiotics exist, the fluorescence of carbon dots is effectively quenched by tetracycline antibiotics, and the content of tetracycline antibiotics in the sample can be detected according to the degree of fluorescence change. Compared with the traditional method, the method for detecting tetracycline antibiotics provided by the present invention is simple and fast, without additional modification of markers, can directly detect samples, and has more advantages in practical applications.

Description

technical field [0001] The invention relates to fluorescent carbon dots, in particular to a yellow fluorescent carbon dot and a preparation method thereof, and the use of the carbon dots in the detection of tetracycline antibiotics. Background technique [0002] Tetracyclines (TCs) antibiotics are a class of broad-spectrum antibiotics produced by actinomycetes, mainly including chlortetracycline, oxytetracycline, and tetracycline. They act bacteriostatically by interfering with bacterial protein synthesis and, at very high concentrations, kill bacteria. Due to its strong penetrating power, long half-life, and broad antimicrobial activity against both Gram-positive and food-borne Gram-negative bacteria, it has long been used as the main antibiotic for anti-infection treatment in humans and animals. But because of its increasing usage, it has been left in water and food. This directly affects human health, induces drug-resistant bacteria in the human body, and then affects t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65G01N21/64
CPCC09K11/65G01N21/6428G01N2021/6432G01N2021/6439
Inventor 贾晶双少敏董川
Owner SHANXI UNIV
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