Fluorescent carbon dots for self-targeting cell nucleus and preparation method and application thereof

A technology of fluorescent carbon dots and cell nuclei, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., to achieve the effects of energy saving, time saving, good solubility and dispersibility, and stable optical properties in the preparation process

Active Publication Date: 2021-07-02
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is still challenging to controlly prepare fluorescent carbon dots with self-targeting organelles using a facile preparation method and apply them to the detection of pH.

Method used

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  • Fluorescent carbon dots for self-targeting cell nucleus and preparation method and application thereof
  • Fluorescent carbon dots for self-targeting cell nucleus and preparation method and application thereof
  • Fluorescent carbon dots for self-targeting cell nucleus and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] Preparation method of carbon dots:

[0031] 1) Weigh 0.17g of copper chloride and 0.1g of tryptophan into a glass beaker, add 20mL of deionized water, stir thoroughly and ultrasonically dissolve for 15 minutes;

[0032] 2) Transfer the reaction mixture to a hydrothermal reaction kettle, place it in an oven, and react at 180°C for 3 hours;

[0033] 3), after the reaction stops, let it stand and cool to room temperature, filter, remove insoluble matter to obtain a dark yellow solution, and filter with a 0.22 μm filter membrane to obtain a pure aqueous solution of carbon dots;

[0034] 4), freeze-drying the carbon dot aqueous solution to obtain a carbon dot solid.

Embodiment 2

[0036] The fluorescent carbon dot prepared in embodiment 1 is characterized, and its transmission electron microscope picture is as follows figure 1 , the carbon dots present monodisperse spherical particles in morphology, and the particle size is about 4.84nm; the ultraviolet absorption spectrum and fluorescence excitation-emission spectrum of the above-mentioned synthesized carbon dots are as follows: figure 2 , showing that the absorption peak of carbon dots is 278nm, and the optimal excitation wavelength and emission wavelength are 369 and 464nm; the XPS spectrum of the synthesized carbon dots is as follows image 3 , which proves that the main elements contained in carbon dots are carbon, hydrogen, oxygen, nitrogen, copper, and chlorine; the infrared spectrum of the synthesized carbon dots is as follows Figure 4 , indicating that the carbon dot has a benzene ring structure and the surface contains functional groups such as amino and carboxyl groups; such as Figure 5 F...

Embodiment 3

[0038] HeLa cells were co-incubated with BR buffer solution with a concentration of 0.7mg / mL carbon dots, diluted acridine orange staining solution, and BR buffer solution with a concentration of 0.7mg / mL carbon dots and diluted acridine orange staining solution. For 40 minutes, the morphology and fluorescence imaging phenomenon of HeLa cells were observed by laser confocal microscope, and the fluorescence photos of the nucleus imaging of HeLa cells were obtained, as shown in Figure 7 . Figure 7 The first row from left to right: (A) dark field (excitation at 488nm) cell map (green), (B) bright field, (C) green overlay of bright field and dark field; Figure 7 The second row from left to right: (D) dark field (405nm excitation) cell map (blue), (E) bright field, (F) blue overlay of bright field and dark field; Figure 7 The third row from left to right: (G) dark field (excitation at 488nm) cell image (green), (H) dark field (excitation at 405nm) cell image (blue), (I) bright...

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Abstract

The invention discloses a self-targeting fluorescent carbon dot to the cell nucleus and its preparation method and application. The carbon dot is prepared by a one-step hydrothermal synthesis method using copper chloride and tryptophan as raw materials; the carbon dot It is a zero-dimensional carbon nanomaterial with a diameter of about 3.3‑7.6nm and can emit bright blue fluorescence. The preparation method of the invention is simple and convenient, and the prepared carbon dots have good water solubility and stable fluorescence properties. In particular, the carbon dots prepared by this method do not require additional modification, can target the nucleus in cell imaging, and have a very sensitive response to intracellular pH. The application is that it can be used as a fluorescent probe to quickly enter cells and nuclei without labeling, so as to realize real-time monitoring of pH fluctuations in acidic living cells.

Description

technical field [0001] The invention relates to the preparation and application of fluorescent carbon dots, in particular to a self-targeting cell nucleus fluorescent carbon dot and its preparation method and application. Background technique [0002] The nucleus is the main organelle for eukaryotic cells to maintain gene integrity and regulate cell functions, and contains a large amount of genetic material, namely DNA. The nucleus is usually the ultimate target for disease treatment after crossing a series of biological barriers. It regulates gene expression and exchanges proteins with the cytoplasm to control cellular processes. The human nuclear genome has 3.2×10 9 base pairs, 2% of which are approximately 30,000 genes. If 30,000 genes are mutated, various gene-related diseases may occur. At present, it has been found that the change of cell nucleus is closely related to the cancerous state of tumor cells. Therefore, nucleus-targeted fluorescence imaging plays an impo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65C01B32/15G01N21/64
CPCC09K11/65C01B32/15G01N21/6402G01N21/6428G01N21/6458G01N21/6486G01N2021/6439
Inventor 郭建花路雯婧郭忠慧张慧林双少敏董川
Owner SHANXI UNIV
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