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Method for extracting 1-deoxynojirimycin from fresh mulberry leaves

A technology of deoxynojirimycin and fresh mulberry leaves, applied in the direction of organic chemistry, etc., can solve the problems of difficult large-scale production and high production cost, and achieve the effects of increasing pH value, increasing yield, and improving dissolution efficiency

Pending Publication Date: 2021-02-02
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese invention patent CN109180563A discloses a method for extracting DNJ from fresh mulberry leaves. Although a high-content DNJ extract is obtained by this method, the process requires grinding and breaking the mulberry leaves. Protease must also be added for enzymatic hydrolysis, which is difficult to produce on a large scale and has high production costs

Method used

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  • Method for extracting 1-deoxynojirimycin from fresh mulberry leaves
  • Method for extracting 1-deoxynojirimycin from fresh mulberry leaves
  • Method for extracting 1-deoxynojirimycin from fresh mulberry leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Get 10kg of fresh mulberry leaves, clean, drain, and cut into strips with a diameter of 3mm; the mulberry leaves after the strips are added into 60L of 70°C, 5% acetic acid extract, and ultrasonic (frequency 40KHz, sound intensity 0.6 W / cm 2 ,) assisted extraction for 30min; then use 60L of pure water to extract twice with the same ultrasonic wave, each time for 20min; combine the extracts, filter with a diatomaceous earth filter, and concentrate in vacuo; dilute the concentrate with purified water to a specific gravity of 1.15, Stand still at 0-4°C for 72 hours; take the supernatant, filter it through an 8 μm filter membrane, pass it through a cation exchange resin, wash it with water until it is colorless, and then elute it with an alkaline aqueous solution until it is colorless, and collect the alkaline eluent; The eluate was concentrated and dried to obtain extract 1.

Embodiment 2

[0030] Get 10kg of fresh mulberry leaves, clean, drain, and cut into strips with a diameter of 4mm; the mulberry leaves after the strips are added into 70L of 60°C extract containing 10% acetic acid, and ultrasonic (frequency 100KHz, sound intensity 1W) / cm 2 ,) assisted extraction for 30min; then use 70L of pure water to extract twice with the same ultrasonic wave, each time for 20min; combine the extracts, filter with a diatomaceous earth filter, and concentrate in vacuo; dilute the concentrate with purified water to a specific gravity of 1.19, Stand still at 0-4°C for 72 hours; take the supernatant, filter it through a 20 μm filter membrane and pass it through a cation exchange resin, then wash it with water until it is colorless, then elute it with an alkaline aqueous solution until it is colorless, and collect the alkaline eluent; The eluate was concentrated and dried to obtain extract 2.

Embodiment 3

[0032] Get 10kg of fresh mulberry leaves, clean, drain, and cut into strips with a diameter of 6mm; add 80L of mulberry leaves with a temperature of 50°C and an extract containing 20% ​​acetic acid, and use ultrasonic waves (frequency 200KHz, sound intensity 2W / cm 2 ,) assisted extraction for 30 minutes; then use 80L of pure water to assist extraction twice with the same ultrasonic wave, each time for 20 minutes; combine the extracts, filter with a plate and frame filter, and concentrate in vacuum; dilute the concentrate with purified water to a specific gravity of 1.22, Stand still at 0-4°C for 72 hours; take the supernatant, filter it through a 60 μm filter membrane and pass it through a cation exchange resin, then wash it with water until it is colorless, then elute it with an alkaline aqueous solution until it is colorless, and collect the alkaline eluent; The eluate was concentrated and dried to obtain extract 3.

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Abstract

The invention discloses a method for extracting 1-deoxynojirimycin from fresh mulberry leaves. The method comprises the following steps: cleaning and draining fresh picked mulberry leaves; cutting thedrained fresh mulberry leaves into strips, and putting the mulberry leaves into an extracting solution containing acetic acid; recovering an extracting solution after ultrasonic treatment; carrying out ultrasonic-assisted extraction on the mulberry leaves twice by taking purified water as an extraction solvent; combining extracting solutions, filtering, concentrating in vacuum, refrigerating, filtering, concentrating and drying to obtain an extract. The method does not need a large amount of organic solvents, and is simple in process, easy to operate and high in extraction rate. The obtainedextract can be used for preparing high-purity 1-deoxynojirimycin.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, in particular to a method for extracting 1-deoxynojirimycin from fresh mulberry leaves. Background technique [0002] Mulberry leaves, also known as iron fan, are the dried leaves of the Moraceae plant Sang (Morus alba L.), which is a traditional Chinese medicine. Traditional Chinese medicine believes that mulberry leaves are cold in nature and sweet in taste, and have the functions of dispelling wind-heat, clearing liver and improving eyesight, cooling blood to stop bleeding, clearing lung and moistening dryness. Mulberry leaf is approved by the Ministry of Health as a dual-use medicine and food. It is rich in resources in my country and has great potential for development. [0003] Studies have found that mulberry leaves contain a special hypoglycemic substance, namely 1-deoxynojirimycin (1-deoxynojirimycin, DNJ). DNJ can competitively inhibit the activity of small intestin...

Claims

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Application Information

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IPC IPC(8): C07D211/46
CPCC07D211/46
Inventor 董志超何际婵
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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