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Anti- P.aeruginosa pharmaceutical composition as well as preparation method and application thereof

A technology of Pseudomonas aeruginosa and composition, applied in antibacterial drugs, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effect of improving antibacterial effect, low price, and good biocompatibility

Inactive Publication Date: 2021-02-02
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of berberine against Pseudomonas aeruginosa

Method used

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  • Anti- P.aeruginosa pharmaceutical composition as well as preparation method and application thereof
  • Anti- P.aeruginosa pharmaceutical composition as well as preparation method and application thereof
  • Anti- P.aeruginosa pharmaceutical composition as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0050] The preparation method of the present invention will be further described in detail in conjunction with specific examples below. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies realized based on the above contents of the present invention are covered within the scope of protection intended by the present invention.

[0051] The experimental methods used in the following examples are conventional methods unless otherwise specified; the reagents and materials used in the following examples can be obtained from commercial sources unless otherwise specified.

Embodiment 1

[0052] Embodiment 1. Berberine and 3,4-dibromomaleimide compatibility

[0053] A solution containing 200 μM berberine and 100 μM 3,4-dibromomaleimide was prepared with pure water or PBS buffer (pH=7.2), and incubated with Pseudomonas aeruginosa in an incubator at 37° C. for 12 hours. Experimental detection bacteriostatic rate ≥ 99.5%.

Embodiment 2

[0054] Embodiment 2. Compatibility of bromoberberine and 3,4-dibromomaleimide

[0055]Use pure water or PBS buffer (pH=7.2) to prepare a solution containing 100 μM bromoberberine and 50 μM 3,4-dibromomaleimide, and incubate with Pseudomonas aeruginosa in an incubator at 37°C for 12 hours . Experimental detection bacteriostatic rate ≥ 99.5%.

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Abstract

The invention provides an anti- P.aeruginosa pharmaceutical composition as well as a preparation method and application thereof. The pharmaceutical composition comprises the following components: (1)berberine and / or halogenated berberine, and (2) 3, 4-dibromomaleimide. Compared with single use of the berberine or the halogenated berberine for resisting P.aeruginosa, the anti- P.aeruginosa pharmaceutical composition has the advantages that after the berberine, the halogenated berberine and the 3, 4-dibromomaleimide adjuvant are compatible, the anti- P.aeruginosa activity of the pharmaceuticalcomposition can be improved by 4 times or above compared with that of raw medicines (the berberine and the halogenated berberine). Due to the fact that the dosage of raw medicines is greatly reduced,the content of the medicines of the berberine and the halogenated berberine contained in the novel preparation can be reduced by 4 times or above compared with the concentration of the raw medicines,and toxicity and various adverse reactions caused by high-dosage medication of various berberine and halogenated berberine are effectively avoided.

Description

technical field [0001] The invention belongs to the field of anti-Pseudomonas aeruginosa, in particular to an anti-Pseudomonas aeruginosa pharmaceutical composition and its preparation method and application. Background technique [0002] Pseudomonas aeruginosa, or P. aeruginosa for short, is a ubiquitous Gram-negative pathogenic bacterium that causes high morbidity in cystic fibrosis patients and immunocompromised patients and mortality. Pseudomonas aeruginosa is considered an opportunistic pathogen, the most common bacterium in nosocomial infections, and its sources are very diverse, including cystic fibrosis, burns, urinary tract infections, and lung infections bacteria. Pseudomonas aeruginosa has low pathogenicity but strong resistance to most antibiotics including aminoglycosides, quinolones and β-lactams. The mechanisms of drug resistance are diverse, including the production of antibiotic inactivating enzymes, inhibition of drug penetration through the cell wall, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61K31/4015A61P31/04
CPCA61K31/4015A61K31/4375A61P31/04A61K2300/00
Inventor 陈懿吴飞鹏李洪峰
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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