HPLC method for detecting saponin in panax notoginseng leaves

A technology of notoginseng saponin and notoginseng leaf, which is applied in the medical field and can solve the problems of less types of notoginseng saponin monomers and lagging detection methods

Inactive Publication Date: 2021-01-29
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection method of saponin monomers in Panax notoginseng leaves is still relatively lagging behind, mainly because there are fewer types of saponin monomers in Panax notoginseng leaves

Method used

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  • HPLC method for detecting saponin in panax notoginseng leaves
  • HPLC method for detecting saponin in panax notoginseng leaves
  • HPLC method for detecting saponin in panax notoginseng leaves

Examples

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Effect test

Embodiment 1

[0032] A HPLC method for detecting saponins from Panax notoginseng leaves, characterized in that it comprises the steps of:

[0033]Step (1), preparation of the test sample solution: dry the leaves of Panax notoginseng in time after harvesting at a temperature of 55°C, grind them into powder, pass through a 45-mesh sieve, and the sieved Panax notoginseng powder Add chloroform, let it stand overnight for 10 hours, filter, discard the supernatant, take the precipitate, add chloroform after the precipitate has dried naturally, filter again after standing for 2 hours, take the precipitate, wait for the precipitate to dry naturally, take the air-dried powder and add Methanol, stand still for 30 minutes, then ultrasonically extract for 20 minutes, let stand for 1 hour, let it cool down to room temperature, finally use methanol for precise quantification, shake slowly, take the liquid after standing for 30 minutes, and filter it with a 0.45 micron filter membrane to obtain the test so...

Embodiment 2

[0039] A HPLC method for detecting saponins from Panax notoginseng leaves, characterized in that it comprises the steps of:

[0040] Step (1), preparation of the test sample solution: dry the leaves of Notoginseng leaves in time after harvesting at a temperature of 60°C, grind them into powder, pass through a 45-mesh sieve, and add the leaves of Notoginseng leaves under the sieve Chloroform, stand overnight for 12 hours, filter, discard the supernatant, take the precipitate, add chloroform after the precipitate has dried naturally, filter again after standing for 3 hours, take the precipitate, wait for the precipitate to dry naturally, take the air-dried powder and add methanol After resting for 40 minutes, ultrasonically extract for 30 minutes, let it stand for 2 hours, let it cool to room temperature, and finally use methanol for precise quantification, shake slowly, and after standing for 40 minutes, take the liquid and filter it with a 0.45 micron filter membrane to obtain ...

Embodiment 3

[0055] A HPLC method for detecting saponins from Panax notoginseng leaves, characterized in that it comprises the steps of:

[0056] Step (1), preparation of the test sample solution: dry the notoginseng leaves in time after harvesting at a temperature of 58°C, grind them into powder, pass through a 45-mesh sieve, and accurately weigh the sieved notoginseng Put 1g of leaf powder in a 50ml Erlenmeyer flask, add 50ml of chloroform, let it stand overnight, filter the solution with filter paper the next day, remove the filtrate, take the precipitate on the filter paper, and after the precipitate is naturally air-dried, add it to 30ml of chloroform again, and let it stand for 2.5 After h, filter again with filter paper, and let the precipitate dry naturally; then take 0.6g of powder and add 50ml of methanol, stand still for 35min, then ultrasonically extract for 25min, let stand for 1.5h, let it cool to room temperature, and finally use methanol to precisely quantify to 50ml, shake ...

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Abstract

The invention relates to an HPLC method for detecting saponin in panax notoginseng leaves, and belongs to the technical field of medicine. The method comprises the following steps of: preparing a testsample solution, preparing a reference substance solution, determining chromatographic conditions, measuring chromatographic peaks, and the like. The HPLC method can accurately measure the contents of seven saponin monomers, including ginsenosides Rb1, Rc, Rb2 and Rb3, notoginsenosides Fa and Fe and gypenoside XVII in panax notoginseng leaves in a short time, meets the related standards of Pharmacopoeia of People's Republic of China in precision, stability, repeatability, minimum detection limit and sample recovery rate, and is rapid, simple, stable and reliable and easy to popularize and apply.

Description

technical field [0001] The invention belongs to the field of medical technology, and relates to an HPLC method for detecting saponins from Panax notoginseng leaves. In particular, it relates to the HPLC method for analyzing and detecting the content of seven kinds of saponin monomers in Panax notoginseng leaves, specifically, it relates to the established HPLC analysis and detection method for the notoginseng saponin monomer quantitative method. Background technique [0002] Triterpenoid saponins are the main pharmacologically active substances of Panax notoginseng (Burk.) F.H.Chen ex C.Chow]. Studies have shown that Panax notoginseng contains both panaxadiol-type saponins and panaxatriol-type saponins. Among dozens of saponins, the saponin monomer is known as ginsenoside Rg 1 , Rb 1 , Rd and notoginsenoside R 1 Mainly, the content accounts for about 80% of the total saponins. Compared with the root of the traditional medicinal part of Panax notoginseng, the medicinal a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/60
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/6052G01N2030/047
Inventor 张广辉贾兵范伟陈庚李晓波李莹宋婉玲杨生超卢迎春刘冠泽张双艳林源
Owner YUNNAN AGRICULTURAL UNIVERSITY
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