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A crop growth-promoting and disease-resistant dse strain and its application

A technology of strains and fungi, applied in the direction of application, plant growth regulators, plant growth regulators, etc., can solve the problems of different interactions, negative effects on plant growth, and inability to promote the growth of another plant, so as to reduce the incidence of disease rate, the effect of promoting rice growth

Active Publication Date: 2022-08-02
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The applicant team has been committed to the research on DSE resources and its application in agricultural and natural environment protection. At present, several strains of DSE fungi with good biological characteristics have been screened out. Although DSE can co-exist with host plants, different species and different The interaction between the strains on different crops is different. Some fungi can promote the growth of one plant but not another. Some fungi can prevent and resist diseases, but some fungi cannot. To the corresponding effect, there are even reports that DSE has a negative impact on the growth of plants

Method used

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  • A crop growth-promoting and disease-resistant dse strain and its application
  • A crop growth-promoting and disease-resistant dse strain and its application
  • A crop growth-promoting and disease-resistant dse strain and its application

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Experimental program
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Effect test

Embodiment 1

[0023] The present invention provides a DSE strain Phialocephala fortinii J2PC4, whose deposit number is CGMCCNO.20236. Belongs to the kingdom of fungi, Phialocephala genus, fortinii species. The DSE strain is preserved in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, with the preservation number of CGMCC NO.20236 and the preservation date of August 10, 2020.

[0024] Morphological observation of strain J2PC4, such as figure 1 As shown, after the strain was cultured on the wort medium medium at 28°C for 14 days, the colonies were nearly round to round, gray, and white villi, and the colonies were 50-55 mm in diameter, and the villi covered the entire plate. In line with the morphological characteristics of the Phialocephala genus, fortinii strains.

Embodiment 2

[0026] Growth-promoting effect on rice:

[0027] (1) Preparation of bacterial liquid: culture the strain on a wort medium plate for about 10 days, transfer it to a potato liquid medium medium for shaking culture (28°C, 120 r / min) for 10-15 days; filter and collect with sterilized gauze Mycelium, rinse with sterile water several times, break the mycelium mass into 5×10 5 The bacterial liquid of cfu / mL is ready for use;

[0028] (2) Cultivation of rice seedlings: The rice seeds are sown in transparent culture cups after germination and whitening, and the seedling substrates in the cups have been sterilized. 30 plants / cup were irrigated with bacterial liquid J2PC4 as the treatment group. Distilled water irrigation was used as a control treatment, and each treatment had three replicates with 3 cups each; rice seedlings were transplanted into strip pots after the second inoculation of J2PC4 strain for 2 to 3 days.

[0029] (3) Inoculation method: On the basis of step (2), when th...

Embodiment 3

[0036] The effect of reducing the incidence of black-streaked dwarf disease in southern rice and resistance to white-backed planthopper:

[0037] (1) Preparation of bacterial liquid: Culture the strain on a wort medium plate for about 10 days, transfer it to a potato liquid medium medium for shaking culture (28°C, 120r / min) for 10-15 days; filter with sterilized gauze Collect the mycelium, rinse with sterile water several times, break the mycelium mass, and make 5×10 5The bacterial solution of cfu / mL was used for later use.

[0038] (2) Rice sowing and inoculation of J2PC4: rice seeds were sown in transparent culture cups after germination and whitening, and the seedling substrates in the bottles had been sterilized, 30 plants / cup, and the treatment group was irrigated with bacterial liquid J2PC4, and the treatment group was not inoculated with J2PC4. The distilled water irrigation of the strain was a control treatment, and three replicates were set for each treatment, and ea...

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Abstract

The invention relates to the field of biotechnology, in particular to the DSE fungal strain J2PC4 and its application. The invention discloses a strain Phialocephala fortinii J2PC4 with a deposit number of CGMCC NO.20236. The strain has the properties of promoting the growth of rice, reducing the incidence of southern rice black-streaked dwarf disease, hindering the growth and reproduction of white-backed planthopper pests, and effectively preventing and controlling banana fusarium wilt. It is a DSE fungus with good growth promotion and disease prevention effects, which can be widely used in the field of biochemical fertilizers and biopesticides, effectively reducing the dependence of agricultural production on chemical pesticides and chemical fertilizers. Compared with the control, it can effectively promote the growth of rice, increase the biomass (dry weight) by 46.43%, effectively reduce the incidence of southern rice black-streaked dwarf disease by 11.70%, and increase the lethality of white-backed planthopper by 369.23%. The reproduction rate of white-backed planthopper was reduced by 26.73%, and the control effect on banana fusarium wilt reached 70.97%.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a crop growth-promoting and disease-resistant DSE strain and its application. 【Background technique】 [0002] Dark septate endophyte (DSE) is one of the typical representatives of plant symbiotic fungi, they can colonize the roots of plants but are not pathogenic to the host, and the hyphae have dark septations and can form in the roots" Microsclerotia" and other structural features. DSE is widely found in nature and can even survive in adversity and extreme environments such as high mountains, polar regions, acidic and dry soils. The applicant team has been devoted to the research on DSE resources and its application in the protection of agricultural natural environment. At present, a number of DSE fungi with good biological characteristics have been screened. Although DSE can coexist with host plants, different species and different The interaction of strains on different crops...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00A01P7/04A01P3/00A01P1/00C12R1/645
CPCA01N63/30C12R2001/645C12N1/145
Inventor 张艳秦碧霞谢玲农倩陈艳露廖仕同曾凤花
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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