Hypsizygus marmoreus GJ5 strain as well as SSR marker primers and application thereof
A technology for labeling primers and crab-flavored mushrooms, applied in the fields of application, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as differences in the quality of crab-flavored mushroom strains, and achieve high accuracy, cost reduction, and detection. short time effect
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Embodiment 1
[0042] Example 1: Development and application of SSR-labeled primers for Mushroom GJ5 strain
[0043] The said GJ5 strain of crab-flavored mushroom is an industrial crab-flavored mushroom strain independently developed by the laboratory. This strain has the advantages of high yield, stable strain properties, high concentration of liquid strains, strong vitality, neat fruiting, and good taste.
[0044] The Gujian white jade mushroom GJ5 strain developed by the present invention is preserved for strains. The preservation unit: China Typical Culture Collection Center; Address: Wuhan, China. Wuhan University; preservation date: September 16, 2020; Hypsizygusmarmoreus GJ5 strain The deposit number is CCTCC M 2020506.
[0045] (1) Sequence analysis and primer design of the SSR markers of the crab-flavored mushroom:
[0046] The genome of the GJ5 strain was extracted for whole genome sequencing, and the obtained genome size was 45.1M. The SSR marker sequence was searched in the geno...
Embodiment 2
[0072] Example 2: SSR-marked fingerprints of fruiting bodies in different parts of No. 5 Gujian Xieweigu No. 5
[0073] The SSR marker in the present invention is based on the 150 simple repeats (SSRs) found on the genomic DNA using the SSRhunter1.3 software after the whole genome sequencing of the crab-flavored mushroom, and specific primers are designed according to its upstream and downstream gene sequences, and the collected After PCR amplification, electrophoresis was performed to obtain polymorphic marker fragments of the crab-flavored mushroom species. SSR marking has the advantages of simple detection method, less requirement for sample volume, no requirement for sampling position, good repeatability, and short detection time. The present invention screens a large number of SSR primers, and finally obtains 5 pairs of primers with high polymorphism and good specificity. The fragment combination obtained by using these 5 pairs of primers can be used for 24 kinds of white...
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