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Application of long-chain acyl carnitine as biomarker of cerebral arterial thrombosis

A technology for ischemic stroke and long-chain acylcarnitine, applied in the field of biomedicine, can solve the problems of unclear regulation and mechanism, etc.

Active Publication Date: 2021-01-26
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether LCAC can be used as a diagnostic marker for ischemic stroke, and its regulatory role and mechanism in the pathological process of ischemic stroke are still unclear

Method used

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  • Application of long-chain acyl carnitine as biomarker of cerebral arterial thrombosis
  • Application of long-chain acyl carnitine as biomarker of cerebral arterial thrombosis
  • Application of long-chain acyl carnitine as biomarker of cerebral arterial thrombosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening of Differential Lipids by UPLC-Orbitrap Mass Spectrometry System

[0038] 1. Instruments and reagents used

[0039] Q-Exactive Plus mass spectrometer (Thermo Scientific)

[0040] UHPLC Nexera LC-30A Ultra High Performance Liquid Chromatograph (SHIMADZU)

[0041] Low-temperature high-speed centrifuge (Eppendorf 5430R)

[0042] Chromatographic column: Waters, ACQUITY UPLC CSH C18, 1.7μm, 2.1mm×100mm column acetonitrile (Thermo Fisher)

[0043] Isopropanol (Thermo Fisher)

[0044] Methanol (Thermo Fisher)

[0045] Ammonium formate (Sigma, 70221)

[0046] 2. Project test process

[0047] 2.1 Preparation of focal cerebral ischemia-reperfusion animal model

[0048]The middle cerebral artery occlusion (MCAO) model of C57BL / 6J mice was prepared by using a polylysine-coated nylon thread plug (model: 1622A4, Beijing Xinong Technology Co., Ltd., China) according to the Longa method. C57BL / 6J mice were fasted for 8 hours before operation, anesthetized mice by intr...

Embodiment 2

[0064] MRM method verifies the high expression of LCAC in the infarct penumbra of mice with ischemic stroke

[0065] 1. Instruments and reagents used

[0066] 13 deuterium isotope-labeled carnitine standards, the article numbers are NSK-B (8 pieces) and NSK-B-G (5 pieces);

[0067] 13 non-isotope-labeled fatty acylcarnitine standard substances, the article numbers are NSK-B-US (8 pieces) and NSK-B-G-US (5 pieces), all purchased from Cambridge Isotope Laboratories, USA (Cambridge Isotope Laboratories, Inc);

[0068] Liquid chromatography-mass spectrometry grade (LC-MS) methanol and acetonitrile were purchased from Fisher Scientific. Liquid chromatography-mass spectrometry grade (LC-MS) formic acid was purchased from Sigma-Aldrich, USA. Other experimental materials were purchased from Shanghai Anpu Experimental Technology Co., Ltd. The main equipment includes Agilent ultra-high performance liquid chromatography (model 1290Infinity II), Agilent tandem quadrupole mass spectrom...

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Abstract

The invention discloses an application of long-chain acyl carnitine as a biomarker for cerebral arterial thrombosis, belongs to the field of biomedicine, and finds that the expression of the long-chain acyl carnitine in ischemic penumbra of a mouse acute cerebral arterial thrombosis model is up-regulated on the basis of a research technology of lipidomics and screening analysis of bioinformatics.Specifically, the invention relates to application of long-chain acyl carnitine in preparation of products for stroke diagnosis, risk assessment, diagnosis and treatment effect assessment of the severity degree of illness, prognosis recovery judgment and the like, early diagnosis, risk assessment and early warning of stroke can be rapidly and effectively carried out, and early diagnosis and earlywarning of stroke can also be rapidly and effectively carried out, and an important basis can be provided for evaluation and prognosis judgment of disease severity and treatment effect in the later period, and a treatment scheme of a doctor is assisted. In addition, the long-chain acyl carnitine disclosed by the invention can also provide a target for screening medicines for preventing and treating cerebral arterial thrombosis and clinical treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of long-chain acylcarnitine as a biomarker of ischemic stroke. Background technique [0002] Ischemic stroke is a common disease worldwide, characterized by high morbidity, disability, and mortality. In recent years, although vascular recanalization methods such as intravenous thrombolysis and mechanical thrombectomy have made some progress in the treatment of ischemic stroke, the proportion of patients who can receive vascular recanalization within the time window is still very low. brought a heavy burden. At present, the diagnosis of acute ischemic stroke mainly relies on CT, MRI and other imaging methods, but CT and MRI scanning are not completely reliable methods. CT has only 16% sensitivity for early ischemic stroke, while for hemorrhagic stroke Stroke had an 89% sensitivity. Twenty percent of ischemic strokes are not detected by MRI scanning techniqu...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86G01N33/92
CPCG01N30/02G01N30/06G01N30/7266G01N30/8665G01N30/8675G01N30/8696G01N33/92G01N2030/045G01N2800/2871G01N2800/52
Inventor 孙秀兰黄新鑫杨进季娟郭若冰施海彬程虹孙涛胡刚
Owner NANJING MEDICAL UNIV
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