Pathogen analysis method and device based on high-throughput sequencing and computer equipment

Abstract

The invention provides a pathogen analysis method and device based on high-throughput sequencing and computer equipment, and the method comprises the steps: obtaining sequencing data of a to-be-analyzed sample, segmenting each sequence of the sequencing data according to K-mer to obtain a plurality of K-mers; performing hash calculation on the plurality of K-mers, and searching genome positions and weights corresponding to all the K-mers of each sequence in a pre-established genome hash table according to the K-mers subjected to hash calculation; calculating the total weight of each sequence according to the weights corresponding to all the K-mers of each sequence; and performing classification analysis according to the genome positions corresponding to all the K-mers of each sequence andthe total weight of each sequence, and determining species attributes of pathogens. According to the method, the species attributes can be determined only by determining the position and the weight ofthe K-mer of each sequence of the sample to be analyzed, and the accuracy is high.

Description

technical field [0001] The present invention relates to the technical field of data processing, in particular to a pathogen analysis method, device, computer equipment and readable storage medium based on high-throughput sequencing. Background technique [0002] With the gradual maturity of next-generation sequencing technology, more and more technologies based on next-generation sequencing are used in the fields of medicine and scientific research. Pathogen detection is a typical application. As we all know, a large part of the diseases that occur in humans are caused by the invasion of external pathogens, so identifying the pathogens has become the key to treating diseases and saving lives. Before next-generation sequencing technology is immature, the most common method is to determine whether the pathogen is a virus or bacteria through blood routine examination, and then use antibiotics or interferon for treatment, but this cannot efficiently target the corresponding pat...

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention provides a pathogen analysis method, device, computer equipment and readable storage medium based on high-throughput sequencing to solve the problem that current high-throughput gene detection methods for pathogens need to repeatedly compare human genomes with different Species genome, resulting in too long analysis time and technical problems of low comparison efficiency

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