Denitrifying bacterium and application thereof

A technology for removing and nitrate nitrogen, applied in the field of denitrifying bacteria, can solve the problems of inability to meet denitrification needs, high cost, and low denitrification efficiency.

Active Publication Date: 2021-01-22
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above denitrification processes not only generally realize the nitrification process and the denitrification process in different reactors, but also 50% of the denitrification processes cannot meet the denitrification requirements due to insufficient carbon sources for denitrification
Among the solutions to the low nitrogen removal efficiency of sewage with low carbon-to-nitrogen ratio (C / N), the addition of carbon sources is simple and effective, but the cost is high

Method used

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  • Denitrifying bacterium and application thereof
  • Denitrifying bacterium and application thereof
  • Denitrifying bacterium and application thereof

Examples

Experimental program
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Embodiment 1

[0020] 1. Isolation, purification and preservation of Pseudomonas JM10A2a

[0021] The samples were collected from the culture pond water of Penaeus vannamei in Guanghai Town Breeding Base (N21°56′31″; E112°46′16″) of Nianfeng Company, Taishan City, Jiangmen City, Guangdong Province. Breeding water sample 1mL, gradient dilution 10 -1 , 10 -2 , 10 -3 and 10 -4 After that, take 200 μL of 10 -2 、10 -3 and 10 -4 The diluted solution was spread on the selective medium used for the isolation and screening of denitrification bacteria, and cultured in a 30°C incubator. Observe the colony morphology with the naked eye, pick single colonies with differences in morphology, streak and purify them separately, transfer the purified colonies to 5mL R2A liquid culture concentration, culture at 30°C, 120rpm, and store them in glycerol tubes for subsequent purification to obtain strains JM10A2a.

[0022] The formulation of the selective medium is as follows: 2.5 g sodium succinate, 2.5 ...

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Abstract

The invention discloses a denitrifying bacterium and application thereof. The collection number of Pseudomonas sp. JM10A2A is stored with number of GDMCC No.61214. The denitrifying characteristic research result of the Pseudomonas sp. JM10A2A indicates that the strain is a heterotrophic nitrification-aerobic denitrification strain, can efficiently remove nitrate nitrogen and ammonium nitrogen in awater body, and can still efficiently remove nitrite nitrogen under the condition of low C / N. Thus, the Pseudomonas sp. JM10A2A has great application potential in the aspect of water body denitrification.

Description

Technical field: [0001] The invention belongs to the technical field of microorganisms and environmental engineering, and in particular relates to a denitrification bacterium and its application. Background technique: [0002] With the development of industry and agriculture, a large amount of nitrogen is discharged into various water bodies, such as oceans, lakes, underground rivers and urban rivers. The nitrogen content is increasing day by day, which poses a serious threat to the ecological environment and human health. [0003] The removal of nitrogen-containing pollutants in water mainly includes three methods: physical, chemical and biological. Among them, microbial denitrification technology overcomes some defects of physical and chemical denitrification processes, and has the advantages of simple operation, wide application range, good treatment effect, Basically no secondary pollution and other advantages, it has become a hot spot in the field of wastewater denitrif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC12N1/20C02F3/34C02F2101/16C02F2101/163C02F2101/166C12R2001/38C12N1/205
Inventor 朱红惠张明霞臧毅李安章陈猛徐帅帅姚青
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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