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A kind of sea bacillus with denitrification function and its application

A technology of bacillus and sea rod, applied in the field of microbiology and environmental engineering

Active Publication Date: 2022-02-11
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • A kind of sea bacillus with denitrification function and its application
  • A kind of sea bacillus with denitrification function and its application
  • A kind of sea bacillus with denitrification function and its application

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Embodiment 1

[0023] 1. Isolation, Purification and Preservation of Bacillus marinum S33H4

[0024] Southwest Indian Ocean deep-sea sediment samples (S38°42′54″; E46°58′6″), two sediment samples were taken, connected to a Erlenmeyer shaker flask containing 100mL enrichment medium, 180rpm, 28°C, carried out Enrichment culture. After culturing for 5 days, transfer the culture solution to a new Erlenmeyer flask containing the enrichment medium at a ratio of 1:100 by volume, at 28°C, 180 rpm, and culture for 5 days for enrichment twice, repeat the above steps for the third time Enrichment. The third enriched culture solution was diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 , take 10 -3 、10 -4 、10 -5 100 μL of the diluted culture solution was spread on the separation medium, and cultured in a constant temperature incubator at 28°C for 3-5 days. The colony shape, color, etc. were observed with the naked eye, and a single colony on the plate was picked and purified by streaking. The puri...

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Abstract

The invention discloses a Bacillus hominis with denitrification function and its application. Marivirgasp. S33H4, the deposit number is GDMCCNo:61709. The Bacillus inhabitants S33H4 of the present invention can effectively remove nitrate nitrogen, ammonium nitrogen and nitrite nitrogen in water bodies, wherein the removal effects of nitrate nitrogen and ammonium nitrogen are all above 90%, In addition, the strain also has a significant removal effect on nitrite nitrogen. This shows that Bacillus coccidioides S33H4 has good application potential in water denitrification.

Description

Technical field: [0001] The invention belongs to the technical field of microbes and environmental engineering, and in particular relates to a sea bacillus with denitrification function and its application. Background technique: [0002] With the rapid development of the domestic aquaculture industry, the high-density farming model is more and more popular. However, while high-density farming meets people's demand for aquatic products, it also causes serious harm to the breeding environment and surrounding ecological environment due to factors such as excessive feed input and frequent use of antibacterial agents. On the one hand, the deterioration of the aquaculture water itself, such as the accumulation of excessive bait, feces and secretions, increases the concentration of ammonium nitrogen and nitrite nitrogen in the water, endangering the healthy growth of aquaculture animals, affecting the production of aquaculture, and improving the quality of aquaculture. cost; on th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/163C02F2101/166C02F2101/16
Inventor 姚雨欣
Owner JINAN UNIVERSITY
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