A kind of sea bacillus with denitrification function and its application
A technology of bacillus and sea rod, applied in the field of microbiology and environmental engineering
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[0023] 1. Isolation, Purification and Preservation of Bacillus marinum S33H4
[0024] Southwest Indian Ocean deep-sea sediment samples (S38°42′54″; E46°58′6″), two sediment samples were taken, connected to a Erlenmeyer shaker flask containing 100mL enrichment medium, 180rpm, 28°C, carried out Enrichment culture. After culturing for 5 days, transfer the culture solution to a new Erlenmeyer flask containing the enrichment medium at a ratio of 1:100 by volume, at 28°C, 180 rpm, and culture for 5 days for enrichment twice, repeat the above steps for the third time Enrichment. The third enriched culture solution was diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 , take 10 -3 、10 -4 、10 -5 100 μL of the diluted culture solution was spread on the separation medium, and cultured in a constant temperature incubator at 28°C for 3-5 days. The colony shape, color, etc. were observed with the naked eye, and a single colony on the plate was picked and purified by streaking. The puri...
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