Zinc-rich candida tropicalis strain and use thereof
A technology of Candida tropicalis and Candida spp. is applied in directions such as application, fungi, and treatment of microorganisms with electricity/wave energy, can solve problems such as being unable to be absorbed, and no public reports have been reported, and achieves improved zinc content, Improved reproductive performance and high productivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0022] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:
[0023] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+ Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed solution;
[0024] The Zn 2+ is zinc sulfate;
[0025] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 30g / L to make the zinc content reach 300μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into conical flasks, 100mL per bottle, sterilized at 120°C for 15 minutes,...
Embodiment 2
[0028] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:
[0029] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed liquid;
[0030] The Zn 2+ is zinc oxide;
[0031] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 50g / L to make the zinc content reach 500μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into conical flasks, 100mL per bottle, sterilized at 120°C for 15min, when the ...
Embodiment 3
[0034] The application method of a strain of zinc-rich Candida tropicalis strain UDY-12 in the preparation of zinc-rich yeast feed additive of the present invention comprises the following steps:
[0035] (1) Preparation of seed solution: insert Candida tropicalis strain UDY-12 into Zn 2+ Erlenmeyer flasks with plate medium, Zn 2 + The mass content is 500μg / mL, cultured at 28°C for 36h, and then inserted with Zn 2+ In the Erlenmeyer flask of YEPD medium, place on a shaker, the shaker speed is 180r / min, and cultivate at 28°C for 60h to obtain a fermentation culture seed liquid;
[0036] The Zn 2+ is zinc sulfate;
[0037] (2) Preparation of fermentation culture: Add zinc sulfate stock solution to the wort medium with a mass sugar concentration of 70g / L to make the zinc content reach 800μg / mL, add 2g of potassium dihydrogen phosphate, and use wort medium to set The capacity is up to 1000mL, divided into Erlenmeyer flasks, 100mL per bottle, sterilized at 120°C for 15 minutes...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com