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Method for quickly screening [alpha]-glucosidase inhibitor in quinoa bran

A technology for glucosidase and screening method, which is applied in the field of compounds of α-glucosidase inhibitors, and can solve the problems of large workload, complicated synthesis process and the like

Active Publication Date: 2021-01-15
中科爱伽(天津)医用食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Such a screening method has a large workload, and the synthesis process is usually cumbersome.

Method used

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  • Method for quickly screening [alpha]-glucosidase inhibitor in quinoa bran
  • Method for quickly screening [alpha]-glucosidase inhibitor in quinoa bran
  • Method for quickly screening [alpha]-glucosidase inhibitor in quinoa bran

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Embodiment 1

[0116] 1. Materials and Methods

[0117] 1.1. Materials

[0118] Quinoa bran is provided by Qinghai Boji Biotechnology Co., Ltd., powdered, white quinoa (Chenopodium quinoa Willd) mechanically removed bran;

[0119] Yeast α-glucosidase (US sigma company, G5003, 1KU), 4-nitrophenyl-α-D-glucopyranoside (Aladdin Reagent (Shanghai) Co., Ltd.), acarbose (Shanghai Yuanye Technology Co., Ltd. Co., Ltd.), other reagents are all domestic analytical pure;

[0120] Chip: BetterWays 3D Carboxylation Chip, Cat: BW38204, BetterWays (China)

[0121] PBS: HyClone PBS buffer, Cat: SH30256.01B, Thermo Fisher (USA)

[0122] Glycine: Millipore Glycine for analysis, Cat: 1042010250, Merck (Germany)

[0123] HCl: Sigma Hydrochloric acid, Cat: H1758-500ML, Merck (Germany).

[0124] 1.2 Instruments

[0125] Berthold bScreen LB 991 biochip analysis system (Biacore T200 system (GEHealthcare, Sweden)); UPLC-Triple-TOF / MS system: AcquityTM ultra high performance liquid chromatography (Waters, USA), T...

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Abstract

The invention discloses a method for quickly screening an [alpha]-glucosidase inhibitor in quinoa bran, which comprises the following steps: capturing compounds with [alpha]-glucosidase inhibition activity in quinoa bran extract by using SPR and UPLC / MS coupling technique, and separating and identifying the structure of each compound by using liquid chromatography and mass spectrometry, and screening out 18 compounds in the quinoa bran successfully, wherein the 18 compounds have [alpha]-glucosidase inhibition activity, and are efficient and rapid.

Description

technical field [0001] The invention relates to the field of α-glucosidase inhibitors, in particular to a method for screening compounds that can be used as α-glucosidase inhibitors in quinoa bran by using SPR and UPLC / MS combined technology. Background technique [0002] α-glucosidase inhibitors (alpha-glucosidase inhibitors, AGI) is a class of drugs used to treat type 2 diabetes, and has become the drug of choice for patients with type 2 diabetes who are poorly controlled by simple diet and insulin for patients with type 1 diabetes. Treatment of choice adjuvant drugs. A large number of clinical studies in recent years have shown that α-glucosidase inhibitors can strictly control the rise in postprandial blood sugar, reduce the stimulation of the high-sugar environment of diabetic patients on the tissues and organs of the body, and delay the transformation of patients with impaired glucose tolerance to type 2 diabetes. It can overcome some shortcomings of traditional hypog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N2030/062
Inventor 董琦王洪伦胡娜
Owner 中科爱伽(天津)医用食品有限公司
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