Application of 1,2,4 triazolo 4,3-B pyridazine derivative in preparation of anti-inflammatory factor storm drugs
A technology of pyridazine derivatives and inflammatory factors, applied in the field of medicine, can solve problems such as large side effects, limited drugs, high blood pressure and osteonecrosis
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[0044] Example 1 C1632 inhibits the expression of various inflammatory factors
[0045] The inventor's team treated A549, Huh-7, THP1 and PBMC cells with C1632, and detected the effect of C1632 on the expression levels of inflammatory factors in HEK293T, A549, and Huh-7: cells were divided at 2 × 10 6 A 6-well plate was plated per well, 2 ml of DMEM complete medium was added to each well, and 240 μM C1632 was added for treatment for 48 h the next day, and the cells were lysed with Trizol to extract RNA for QRT-PCR analysis.
[0046] C1632 treatment of THP1 cells: THP1 cells by 5 × 10 5 Each well was seeded with 12-well plate, 1 ml of PRMI1640 complete medium per well, and PMA with a final concentration of 100 ng / μl was added to induce cell differentiation. The next day, the medium was changed to fresh medium and C1632 was added for 2 days of treatment. Add LPS or TNF-α with a final concentration of 100 ng / ml to stimulate for 8~12 h, collect the cell culture supernatant for L...
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