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A gas chromatography mass spectrometry detection system and method

A technology of gas chromatography mass spectrometry and detection system, which is used in measurement devices, instruments, scientific instruments, etc.

Active Publication Date: 2022-07-15
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention can not only solve the problem of specific component analysis in the complex matrix of microbial metabolism, but also can be used for the analysis of trace impurities in raw materials and fine chemicals, as well as the analysis of complex active substances and optical isomers

Method used

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  • A gas chromatography mass spectrometry detection system and method
  • A gas chromatography mass spectrometry detection system and method
  • A gas chromatography mass spectrometry detection system and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] like figure 1 As shown, the gas chromatography mass spectrometry detection system includes: a first column box 1, a second column box 2, a heart cutting unit 3, a three-way valve 4, a mass spectrometer interface 5, and a sample inlet 6.

[0036] The first column box chromatographic column sampling end and the second column box chromatographic column sampling end are respectively connected to the first interface and the second interface of the heart cutting unit; the first column box chromatographic column and the second column box chromatographic column are connected respectively. of different polarities.

[0037] The first interface of the three-way valve is connected to the third interface of the central cutting unit, the second interface is connected to the sampling section of the second column box chromatographic column, and the third interface is connected to the mass spectrometer through the mass spectrometer interface.

Embodiment 2

[0039] like figure 1 As shown, the sample to be tested enters the first column box chromatographic column through the injection port, enters the second column box chromatographic column through the first interface of the heart-cutting unit and the second interface of the heart-cutting unit, and then passes through the three-way valve and the mass spectrometer in turn. The instrument interface enters the mass spectrometer.

[0040] like figure 2 As shown in the single-column workflow of the gas chromatography mass spectrometry detection system, the sample to be tested enters the first column box chromatographic column through the injection port, enters through the first interface and the third interface of the heart cutting unit, and then passes through the three-way valve and the mass spectrometer in turn. The instrument interface enters the mass spectrometer.

[0041] like image 3 As shown, the gas chromatography mass spectrometry detection system backflush work flow, t...

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Abstract

The invention relates to the technical field of detection of complex samples of microbial metabolism. The present invention provides a gas chromatography mass spectrometry detection system, which comprises: a first column box chromatographic column 1, a second column box chromatographic column 2, a central cutting unit 3, a three-way valve 4, a mass spectrometer interface 5, and a sample inlet 6 . The invention realizes accurate detection of trace secondary metabolites in microbial fermentation broth, accurately identifies target compounds in complex samples with complex substrates, can effectively reduce the pollution of complex substrates to instruments, especially ion sources, and maintains high sensitivity of the instruments. Eliminate adverse effects such as ghost peaks.

Description

technical field [0001] The present invention relates to the technical field of detection of complex samples of microbial metabolism. Background technique [0002] Gas chromatography-mass spectrometry, referred to as gas mass spectrometer (GCMS), is currently the most widely used mainstream separation and detection system. So far, GCMS has been widely used in food, medicine, chemical industry, inspection and monitoring and other fields. It not only participates in the formulation of national standards and pharmacopoeia methods, but also plays an important supporting role in the field of scientific research. Intelligent, specialization and other characteristics. However, there are still limitations in the development of methods for the effective separation of microbial metabolism of complex organic samples, especially in the field of scientific research. There is no universally tried research method for trace or complex structural components in complex matrices. The characte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/52G01N30/54G01N30/60
CPCG01N30/88G01N30/06G01N30/52G01N30/54G01N30/6043G01N2030/062G01N2030/8813
Inventor 柳国霞蔡真张君丽唐双焱
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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