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Preparation and application of microbial agent for degrading quinclorac

A microbial agent and microbial technology, applied in the field of agricultural environmental soil remediation, can solve the problems of inhibiting the normal growth of crops, reducing production, and cutting off production, achieving the effects of low cost, promoting crop growth, and improving soil

Active Publication Date: 2020-12-29
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these crops are very sensitive to chloroquine phosphate, which will seriously inhibit the normal growth of crops, resulting in reduced or even extinction of production
At present, there is a temporary lack of in-situ remediation agents for chloroquine phosphate-contaminated soil, which can not only solve the problem of soil pollution, but also reduce or eliminate the inhibition of crop growth caused by chloroquine phosphate residues, which has become an urgent problem to be solved

Method used

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  • Preparation and application of microbial agent for degrading quinclorac
  • Preparation and application of microbial agent for degrading quinclorac
  • Preparation and application of microbial agent for degrading quinclorac

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A microbial soil remediation agent is prepared by inoculating a microbial agent on a PDA medium to grow spores, and then inoculating it on a glucose-containing co-metabolism medium for fermentation; the microbial agent is Paecilomyces lilacinus.

[0037] The preparation method of microbial soil remediation agent:

[0038] (1) Inoculate the slant of the preserved strain Paecilomyces lilacinus GFDZ1 on the PDA solid medium, and culture it in a constant temperature incubator at 30°C for 5 days. After a large number of spores grow, add a small amount of sterilized inorganic salts to the plate culture medium, and scrape off the spores growing on the plate, filter with four layers of gauze to remove the mycelium, obtain the spore suspension, measure the absorbance of the spore suspension with a spectrophotometer, and make the suspension OD 600 = 3;

[0039] (2) The spore suspension is added to a co-metabolism medium with a certain inoculum amount for fermentation to obtain t...

Embodiment 2

[0045] The other components are the same as in Example 1, only co-metabolism medium with different components is used, as follows: ① Inorganic salt medium + 0.5% glucose (m / v, the unit is g / mL).

[0046] Among them, the formula of inorganic salt medium: K 2 HPO 4 1.0-1.5g, KH 2 PO 4 1.0-1.5g, NaCl 0.5-1.0g, MgSO 4 ·7H 2 O 0.2-0.5g, FeCl 3 0.05-0.07g, CaCl 2 0.02-0.05g, NH 4 NO 3 1.0-1.5g.

Embodiment 3

[0057] Effects of different inoculation volumes on the degradation of quinclorac by strain GFDZ1

[0058] With 2%, 4%, 8%, 12%, 16%, 20% inoculum size, the spore suspension was added to 50mL sterile co-metabolism medium (inorganic salt medium+0.5% glucose) and dichloroquine Acid concentration is in the 150mL Erlenmeyer flask of 50mg / L, is placed in 30 DEG C, cultivates 5d in the constant temperature shaker of 150r / min, after the completion of the cultivation, take a sample, detect the content of quinclorac in the sample with liquid chromatography, Adding the corresponding concentration of quinclorac and no spore suspension were set as control treatments, and each treatment was replicated three times.

[0059] The effects of different inoculum volumes on the degradation of quinclorac by strain GFDZ1 were as follows: figure 1 Shown, test result shows, when inoculum size is at 2%-8%, the degradation rate of quinclorac and the inoculum size of spore suspension present positive co...

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Abstract

The invention discloses the preparation and application of a microbial agent for degrading quinclorac. The microbial soil remediation agent comprises Paecilomyces lilacinus, and has relatively good degradation effects on quinclorac. The strain can degrade mulching films and prevent and treat rhizosphere nematode, multiple effects of the strain can be exerted when the strain is used to solve the problem of residual phytotoxicity of quinclorac, and no environmental risk exists. The microbial agent is a pure microbial preparation, and is high in safety and free of secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of agricultural environment soil restoration, and in particular relates to the preparation and application of a dichloroquine phosphoric acid degrading microorganism. Background technique [0002] In recent years, there have been irregular drug use behaviors such as blind drug use and excessive drug use, which not only led to an increase in crop injury incidents, but also caused soil pollution. In order to restore the environment, it is imminent to control the land polluted by pesticides. [0003] Quinclorac, whose trade name is Kuaishabai, Shabailing or Shenhoe, is a hormone-type herbicide whose target is the synthetic hormone in plants. Mainly by interfering with the enzyme activity regulated by hormones in plants, it hinders the growth and metabolism of organisms, making them unable to proceed normally, resulting in the phenomenon of leaves becoming smaller, distorted, darker in color, and biomass reduce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10A01N63/30A01P5/00C09K17/14C12R1/79C09K101/00
CPCC12N1/14B09C1/105A01N63/30C09K17/14B09C2101/00C09K2101/00C12R2001/79C12N1/145
Inventor 顾文杰杨少海卢钰升解开治王丹徐培智石超宏孙丽丽
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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