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Injectable biological adhesive, preparation method and application thereof

A technology of biological adhesive and oxidative cross-linking agent, applied in the field of polymer materials and biological materials, to achieve the effect of broad application prospects, no cytotoxicity, and excellent tissue adhesion performance

Active Publication Date: 2020-12-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many studies on bionic bioadhesives, how to make them solidify and adhere quickly according to different needs such as wound size and location, and at the same time make them have high adhesion performance is still a difficult point to overcome.

Method used

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  • Injectable biological adhesive, preparation method and application thereof
  • Injectable biological adhesive, preparation method and application thereof
  • Injectable biological adhesive, preparation method and application thereof

Examples

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preparation example Construction

[0037] The preparation method of the oxidized dextran involved in the following examples is as follows:

[0038] Weigh 4g dextran (DE, M w =2×10 3 ) was dissolved in 80mL deionized water, and 0.244g of sodium periodate (NaIO 4 ), after reacting at room temperature in the dark for 12 hours, ethylene glycol was added to terminate the reaction and dialyzed in deionized water to remove unreacted small molecules until there was no iodate in the dialysate. First take 1mL of dialysate and add 1mL of 1wt% AgNO to it 3 Solution, and there is no precipitation, which proves that the unreacted periodate has been completely removed, and then the liquid in the dialysis bag is freeze-dried to obtain a white powder modified macromolecular ODE 5 , and their degree of oxidation was 5.1%, respectively.

[0039] It should be noted that when NaIO 4 The amount of oxidized dextran prepared by 0.488, 0.976, 1.464, 1.952 is recorded as ODE 10 , ODE 20 , ODE 30 , ODE 40 , the degree of oxidation...

Embodiment 1

[0042] Oxidized dextran-dopamine-chitosan-sodium periodate (ODE-DA-CS-IO 4 - ) preparation of double cross-linking binder:

[0043] Take 190μL 10wt% CS aqueous solution, and add 17.77mg dopamine to it, shake and mix to form a mixed solution; then take 1mL 10wt% ODE 40 PBS solution, and to which was added 2.48mg of NaIO 4 , mix well to form a mixed solution; mix the two mixed solutions (wherein, ODE 40 Aldehyde group in CS, amino group in CS, amino group in DA and NaIO 4 The ratio is 2:1:1:0.1), observe the time of gel formation, and the obtained hydrogel is recorded as ODE-DA-CS-IO 4 - (10mol%) hydrogel binder.

[0044] It should be noted that when taking NaIO 4 The mass of 4.96mg and 9.92mg of the prepared hydrogel is recorded as ODE-DA-CS-IO 4 - (20mol%), ODE-DA-CS-IO 4 - (40mol%) hydrogel binder.

[0045] The schematic diagram of its preparation is as figure 1 (c) shown.

Embodiment 2

[0047] Oxidized dextran-dopamine-chitosan-iron (ODE-DA-CS-Fe 3+ ) preparation of double cross-linking binder:

[0048] Take 190μL of 10wt% CS solution, and add 17.77mg of dopamine to it, shake and mix to form a mixed solution; then take 1mL of 10wt% ODE 40 PBS solution, to which 1.88mg FeCl was added 3 , mix well to form a mixed solution; mix the two mixed solutions (wherein, ODE 40 Aldehyde group in CS, amino group in CS, amino group in DA and FeCl 3 The molar ratio is 2:1:1:0.1), observe the time of gel formation, and the obtained hydrogel is recorded as ODE-DA-CS-Fe 3+ (10mol%) hydrogel binder.

[0049] It should be noted that when the amount of cross-linking agent ferric chloride was 3.76mg and 3.56mg respectively, the prepared bioadhesive materials were recorded as ODE-DA-CS-Fe 3+ (20mol%) and ODE-DA-CS-Fe 3+ (40mol%).

[0050] The schematic diagram of its preparation is as figure 1 (d) shown.

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Abstract

The invention discloses an injectable biological adhesive, a preparation method and application thereof, and belongs to the technical field of high polymer materials and biological materials. According to the preparation method, saccharide macromolecules, namely glucan and chitosan oligosaccharide, with good biocompatibility are selected as matrixes and mixed and dispersed in an aqueous solution with dopamine and an oxidative cross-linking agent, and rapid gelatinization is performed to obtain the multi-cross-linked biological adhesive. The biological adhesive material obtained by the invention has the advantages of injectability, convenience in practical application and operation, capability of being applied to skin tissues, capability of being injected to the required amount according tothe required adhesion size and the like, high mechanical strength, no cytotoxicity and excellent tissue adhesion performance, can be applied to adhesion of various materials such as animal epidermis,in-vivo tissues and organs, glass, metals and oxides thereof, sealants, plastics and the like in clinical operations, and has wide application prospects in the fields of tissue adhesion and repair, hemostasis, packaging, sealing and the like in clinical medicine.

Description

technical field [0001] The invention specifically relates to an injectable bioadhesive and its preparation method and application, belonging to the technical field of polymer materials and biomaterials. Background technique [0002] Tissue wounds caused by traffic accidents, diseases, wars, surgical operations, etc. all need to be repaired. In actual treatment, the shape of the wound and the degree of injury of the patient are different, and the shape of the hydrogel adhesive required for tissue repair is different. However, the externally implanted hydrogel adhesive is limited by the shape of the treatment area, which cannot match well with irregularly shaped damaged tissue sites, which greatly affects the repair efficiency. Therefore, it is very important to ensure that the adhesive material is not limited by the shape of the treatment area and still has a good tissue repair effect. Injectable hydrogel is an in situ gelling scaffold material, its physical state can be con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09J105/08C09J105/02A61L24/08A61L24/00C08J3/24C08J3/075C08L5/08C08L5/02
CPCC09J105/08C09J105/02A61L24/001A61L24/0031A61L24/08C08J3/246C08J3/075A61L2400/06C08J2305/08C08J2405/02C08J2305/02C08J2405/08C08L2205/02C08L5/08C08L5/02
Inventor 施冬健杜德焰石畅章朱迎倪忠斌陈明清
Owner JIANGNAN UNIV
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