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Hyaluronic acid-based modified gliadin nanoparticles as well as preparation method and application thereof

A gliadin and nanoparticle technology, applied in the directions of pharmaceutical formulations, organic active ingredients, cosmetic preparations, etc., can solve problems such as application limitations, multiple emulsion thermodynamic instability, etc., achieve simple preparation methods, improve bioavailability, Enhanced solubility and emulsifying properties

Active Publication Date: 2020-12-25
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its application is greatly limited due to the thermodynamically unstable properties of multiple emulsions due to the flocculation, coalescence and migration of inner and outer aqueous phase components of oil droplets.

Method used

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  • Hyaluronic acid-based modified gliadin nanoparticles as well as preparation method and application thereof
  • Hyaluronic acid-based modified gliadin nanoparticles as well as preparation method and application thereof
  • Hyaluronic acid-based modified gliadin nanoparticles as well as preparation method and application thereof

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preparation example Construction

[0029] One aspect of the embodiments of the present invention provides a method for preparing hyaluronic acid-based modified gliadin nanoparticles, which includes:

[0030] The first mixed reaction system containing gliadin, EGCG, sodium hydroxide, ethanol and water was reacted at 25°C for 24h to obtain a gliadin-EGCG covalent complex, and then the gliadin-EGCG covalent complex was made to The second mixed reaction system of the valence complex, sodium carbonate, ethanol and water was reacted at 25°C for 4 hours to obtain hollow gliadin-EGCG nanoparticles;

[0031] The third mixed reaction system comprising hyaluronic acid, 1,3-carbodiimide, dimethylaminopyridine, DMSO and water was activated at 25° C. for 1 h, and then EGCG was added to the third mixed reaction system, and the React at 60-65°C for 4 hours to obtain a hyaluronic acid-EGCG covalent complex;

[0032] And, the fourth mixed reaction system comprising the hollow gliadin-EGCG nanoparticles, hyaluronic acid-EGCG cov...

Embodiment 1

[0103] (1) Preparation of hollow gliadin-EGCG nanoparticles

[0104] Dissolve gliadin and EGCG in 80% (v / v) ethanol solution respectively, adjust the pH value to 9.0 with 0.1 mol / L sodium hydroxide solution after mixing, and react with continuous stirring at 25°C for 24h, After the reaction was completed, the obtained mixed solution was ultrasonically dialyzed in a water bath for 24 hours, during which the dialysate was replaced 8 times to remove free EGCG, and finally the solution was freeze-dried to obtain a gliadin-EGCG covalent complex;

[0105]Dissolve 2g of gliadin-EGCG covalent complex in 100mL ethanol solution (volume fraction 80%), and stir at 800rpm for 2h, let it stand until completely dissolved, and simultaneously mix 2wt% sodium carbonate aqueous solution and absolute ethanol by Mix uniformly at a volume ratio of 3:7 to form an ethanol suspension of sodium carbonate, and mix the above suspension with a gliadin-EGCG covalent complex solution at a volume ratio of 1:...

Embodiment 2

[0116] (1) Preparation of hollow gliadin-EGCG nanoparticles

[0117] Dissolve gliadin and EGCG in 80% (v / v) ethanol solution respectively, adjust the pH value to 9.0 with 0.1 mol / L sodium hydroxide solution after mixing, and react with continuous stirring at 25°C for 24h, After the reaction was completed, the obtained mixed solution was ultrasonically dialyzed in a water bath for 24 hours, during which the dialysate was replaced 8 times to remove free EGCG, and finally the solution was freeze-dried to obtain a gliadin-EGCG covalent complex;

[0118] Dissolve 2g of gliadin-EGCG covalent complex in 100mL ethanol solution (volume fraction 80%), and stir at 800rpm for 2h, let it stand until completely dissolved, and simultaneously mix 2wt% sodium carbonate aqueous solution and absolute ethanol by Mix uniformly at a volume ratio of 3:7 to form an ethanol suspension of sodium carbonate, and mix the above suspension with a gliadin-EGCG covalent complex solution at a volume ratio of 1...

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Abstract

The invention discloses hyaluronic acid-based modified gliadin nanoparticles as well as a preparation method and application thereof. The method for preparing the W2 / O2 / (O1 / W1) type multiple emulsionfrom the nanoparticles comprises the following steps: mixing polyglycerol ricinoleate with corn oil at 65 DEG C to form a first oil phase, mixing a mixed solution of sodium chloride and gelatin with the obtained first oil phase, and performing homogenizing by adopting high-pressure microjet to obtain a W2 / O2 type primary emulsion; mixing a second oil phase with the nanoparticle suspension, and performing high-shear treatment to obtain an O1 / W1 type Pickering emulsion; and mixing the W2 / O2 type primary emulsion with the O1 / W1 type Pickering emulsion by adopting a high-pressure homogenization technology, so as to prepare the W2 / O2 / (O1 / W1) type multiple emulsion. The preparation method of the multiple emulsion is simple, mild in reaction condition and suitable for industrial production, and can be applied to the fields of foods, medicines, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field, and in particular relates to a hyaluronic acid-based modified gliadin nanoparticle and its preparation method and application, in particular to a hollow gliadin-EGCG / hyaluronic acid-EGCG nanoparticle and its Preparation method, and hollow gliadin-EGCG / hyaluronic acid-EGCG nanoparticles in the preparation of W 2 / O 2 / (O 1 / W 1 ) Application in multiple emulsions. Background technique [0002] Epigallocatechin gallate (EGCG) has excellent antibacterial and antioxidant activities, can scavenge free radicals, and is widely used as a functional food additive. However, the multiple phenolic hydroxyl groups contained in polyphenols make them unstable to light, high temperature and alkaline conditions, and their bioavailability is also reduced. Many studies have shown that the covalent attachment of polyphenols to biopolymers may increase their physical stability, antioxidant activity, and bioavailability. ...

Claims

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Application Information

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IPC IPC(8): A23L29/00A61K8/06A61K8/49A61K8/64A61K8/73A61K9/113A61K31/353A61K47/61A61K47/69A61Q19/00
CPCA23L29/035A23L29/015A23L29/03A61K9/113A61K8/066A61K47/6931A61K31/353A61K8/498A61K8/64A61K8/735A61K47/61A61Q19/00
Inventor 章宝孟然李小龙刘文杰
Owner HEFEI UNIV OF TECH
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