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Detection kit and detection method for mycoplasma pneumoniae antigen of children

A technology of mycoplasma pneumoniae and detection kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of single diagnosis method of mycoplasma pneumoniae pneumonia in children, and achieve the effect of effective detection method and single diagnosis method

Pending Publication Date: 2020-12-22
丁圣刚 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the deficiencies in the above-mentioned background technology, the purpose of the present invention is to provide a detection kit for mycoplasma pneumoniae antigen in children, to solve the problem that the diagnosis method of mycoplasma pneumoniae pneumonia in children is relatively single in the prior art, and to provide a more direct method for the detection of mycoplasma pneumoniae in humans. , an effective detection method

Method used

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  • Detection kit and detection method for mycoplasma pneumoniae antigen of children
  • Detection kit and detection method for mycoplasma pneumoniae antigen of children
  • Detection kit and detection method for mycoplasma pneumoniae antigen of children

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Experimental program
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Effect test

Embodiment 1

[0029] A detection kit for mycoplasma pneumoniae antigen in children, the detection kit is provided with: an ELISA plate pre-coated with a known calprotectin antibody, an enzyme conjugate working solution, a positive quality control, a negative quality control, and a sample diluent , Biotinylated antibody working solution, chromogenic reagent, concentrated washing solution and stop solution;

[0030] The calprotectin antibody - anti-human S100 calcium-binding protein A8, anti-human S100 calcium-binding protein P, anti-human S100 calcium-binding protein A9 or anti-human S100 calcium-binding protein A12;

[0031] Positive quality control - Mycoplasma pneumoniae positive serum; Negative quality control - healthy negative serum;

[0032] Stop solution - 1mol / L H 2 SO 4 solution;

[0033] Concentrated washing solution - PBS-Tween buffer;

[0034] Chromogen - TMB.

Embodiment 2

[0036] 1. Materials and methods

[0037] 1.1 Research object

[0038] Case group: 100 patients who were admitted to the Pediatrics Department of the First Affiliated Hospital of Anhui Medical University from September 2018 to June 2019 were selected, including 42 females and 58 males. The average age of the patients was 5.38 years old. According to the diagnosis of Mycoplasma pneumoniae pneumonia in children Standards are divided. Control group: 100 healthy children who underwent physical examination in outpatient clinics during the same period, including 47 females and 53 males, with an average age of 5.21 years.

[0039] 1.2 Method

[0040] 2ml of venous blood was collected, placed in a vacuum blood collection tube treated with anticoagulant, and the serum was separated by centrifugation, and the serum calprotectin content was detected by Elisa.

[0041] 1.3 Statistical Analysis

[0042] Statistical SPSS software was used for data processing and clinical analysis. The me...

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Abstract

The invention discloses a detection kit and a detection method for a mycoplasma pneumoniae antigen of children. The detection kit disclosed by the invention is used for determining the content of calprotectin in a sample by applying an enzyme-linked immunoassay method. An enzyme conjugate is incubated, washing is conducted to remove unconjugate, and after thorough washing, a substrate TMB is addedfor color development; and TMB is converted into blue under the catalysis of peroxidase, and is converted into final yellow under the action of acid. The higher the calprotectin concentration in thesample to be detected is, the more inhibited the combination of the labeled antigen and the antibody is, and the lighter the color development is. The developing depth is positively correlated with the enzyme amount, and is negatively correlated with the content of calprotectin to be detected in the sample. A microplate reader is used for measuring absorbance at the wavelength of 450 nm, the concentration of a sample is calculated, then the calprotectin content in the sample is judged, a more direct and effective detection mode is provided for detection of the mycoplasma pneumoniae of children, and the problem that in the prior art, a diagnosis method for the mycoplasma pneumoniae of children is single is solved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection kit and detection method for childhood mycoplasma pneumoniae antigen. Background technique [0002] Mycoplasma pneumoniae pneumonia (MPP), also known as primary atypical pneumonia, cold agglutination-positive pneumonia, is a type of atypical pneumonia, which is caused by mycoplasma (MP) infection, showing interstitial pneumonia and bronchiolitis-like changes , accounting for 10% to 40% of community-acquired pneumonia (CAP) in children. Symptoms vary in severity. Most of the onset is not urgent, with fever, anorexia, cough, chills, headache, sore throat, substernal pain and other symptoms, with fever and cough as the main manifestations. The body temperature is between 37°C and 41°C, most of which are around 39°C. It can be persistent or flaccid, or only low-grade fever, or even no fever. Most of the coughs are severe, initially dry cough, followed by sputum (occ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/535
CPCG01N33/56933G01N33/543G01N33/535
Inventor 丁圣刚王旺沈兵
Owner 丁圣刚
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