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Ratiometric fluorescent probe and application thereof in penicillamine detection

A ratiometric fluorescent probe, penicillamine technology, used in fluorescence/phosphorescence, measurement devices, luminescent materials, etc., can solve problems such as signal uncertainty, and achieve the effect of reducing interference and improving the range of dynamic response.

Pending Publication Date: 2020-12-18
ANHUI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the single-emission signal fluorescence sensor itself has some defects, such as quantum dot nanoprobes are easily interfered by many variable or difficult to quantify factors such as probe concentration, polarity, temperature, environmental pH value, stability, etc. signal uncertainty

Method used

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  • Ratiometric fluorescent probe and application thereof in penicillamine detection
  • Ratiometric fluorescent probe and application thereof in penicillamine detection
  • Ratiometric fluorescent probe and application thereof in penicillamine detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of Au clusters is as follows:

[0018] Precisely prepare 0.01M chloroauric acid solution (dissolve 1g of chloroauric acid in deionized water, and dilute to volume in a 250mL volumetric flask); then prepare 200mL of 1M NaOH solution for later use. Add 10mL of deionized water to a 50mL round bottom flask, weigh 0.0544g MUA (11-mercaptoundecanoic acid) and add it to the round bottom flask. Completely dissolved, stop adding NaOH. Then slowly add 6250 μL of previously prepared HAuCl while stirring 4 Solution, if flocs appear, stir for half an hour, if the precipitate does not disappear, add NaOH solution (1M) dropwise until the solution becomes clear, and stir at room temperature for 24h. After the reaction, the solution was dialyzed for 24 hours. The obtained solution was colorless under sunlight, and showed a strong orange-red light under 312nm ultraviolet light, which was the Au cluster solution. Before use, it was centrifuged at 10,000 rpm for 10...

Embodiment 2

[0020] The preparation method of phenolic resin is as follows:

[0021] 0.05mmol of phenol and 0.025mmol of hexamethylenetetramine (HMT) were dispersed in 22ml of ultrapure water. After fully dissolving, they were transferred to a hydrothermal reaction kettle and reacted at a constant temperature of 160°C for 4h. The reaction product was stored at low temperature. The obtained phenolic resin aqueous solution showed blue-green light under 312nm ultraviolet light. Before the experiment, the tank of the reactor was filled with concentrated HNO with a volume ratio of 10:1 3 with H 2 After soaking the mixture of O for 4 hours, add ultrapure water to wash at a constant temperature of 140 degrees for 4 hours, and finally dry it. Depend on Figure 7 , The phenolic resin of the present invention is evenly distributed and uniform in size.

Embodiment 3

[0023] The construction method of the solution system for ratiometric fluorescence detection of penicillamine is as follows:

[0024] ① Accurately prepare 10mM penicillamine, draw 1000μL of 10mM penicillamine and 9.0ml deionized water to prepare 1.0mM penicillamine, and keep it for future use.

[0025] ②Accurately configure 10mM Cu(NO 3 ) 2 solution, use a 1000μL pipette to draw 1000μL of 10mM Cu(NO 3 ) 2 The solution was prepared with 9.0mL of deionized water to make 1.0mM Cu(NO 3 ) 2 solution, keep it.

[0026] ③ Add 2mL of water, 1mL of Au cluster solution and 1mL of phenolic resin aqueous solution into a test tube and keep it for later use.

[0027] ④ Add 2 mL of water and 100 μL of phenolic resin-Au cluster mixed solution to the cuvette, and add 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 μL in sequence 1mM Cu(NO3)2 solution, so that the concentration of Cu(NO3)2 in the detection system reaches 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14μM , and recorded ...

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Abstract

The invention discloses a ratiometric fluorescent probe and application thereof in penicillamine detection. A ratio type fluorescence sensor has two emission wavelengths, and quantifies the content ofa target object according to the relationship between the ratio of fluorescence intensities at the two wavelengths and the concentration of an analyte. By establishing an internal standard, a self-adjusting function is achieved, and the interference of variable or difficultly quantitative factors on experiments and results is greatly weakened. Moreover, along with the change of the concentrationof the analyte, the color of the ratio probe also changes step by step, so the analyte is visually detected, and semi-quantitative and qualitative analysis of the analyte can be realized according tothe color of the probe.

Description

technical field [0001] The invention relates to a ratio fluorescent nanometer probe phenolic resin-Au cluster-Cu 2+ The detection system and its construction also relate to the application of a ratio fluorescent nanometer probe to detect penicillamine. Background technique [0002] Penicillamine (D-Penicillamine, D-PA) chemical name 3,3-dimethyl-(D)-cysteine, is a kind of amino acid containing mercapto group, which has the ability to chelate metal ions. Cu 2+ effect is particularly prominent. Therefore, it has outstanding ability in the treatment of hepatolenticular degeneration (HLD, an abnormal copper metabolism disease), heavy metal poisoning and so on. On the other hand, the sulfhydryl group of D-penicillamine can decompose rheumatoid factor belonging to macroglobulin due to the breakage of disulfide bonds, thereby reducing the level of serum rheumatoid factor. Based on this, penicillamine has been widely used in autoimmune diseases such as scleroderma and rheumatoid...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/06C09K11/58
CPCG01N21/643G01N21/6402C09K11/06C09K11/58G01N2021/6432C09K2211/1425
Inventor 姚永杰张奎
Owner ANHUI UNIVERSITY OF TECHNOLOGY
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