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Lactobacillus paracasei capable of inhibiting abnormal proliferation of HaCaT cells

A technology of Lactobacillus and side cheese, applied in the field of microorganisms, can solve the problems of obvious adverse reactions

Active Publication Date: 2020-12-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, it may be possible to start with lactic acid bacteria, by constructing a psoriasis-like pathological model, and looking for lactic acid bacteria that can effectively alleviate the condition of psoriasis, so as to find new drugs or new methods for the treatment of psoriasis, so as to overcome the existing therapeutic drugs and methods. Defects such as obvious adverse reactions

Method used

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  • Lactobacillus paracasei capable of inhibiting abnormal proliferation of HaCaT cells
  • Lactobacillus paracasei capable of inhibiting abnormal proliferation of HaCaT cells
  • Lactobacillus paracasei capable of inhibiting abnormal proliferation of HaCaT cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: the acquisition of Lactobacillus paracasei

[0060] Specific steps are as follows:

[0061] 1. Screening

[0062] Taking human feces from Wuxi, Jiangsu as samples, the samples were pretreated and stored in 20% glycerin in a -80°C refrigerator. After taking out and thawing, mix the samples and add 0.5mL samples to 4.5mL to contain 0.05 Gradual dilution of 9 g / L normal saline with g / L cysteine, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, and pick typical colonies of Lactobacillus paracasei on the MRS solid Purify by streaking on the culture medium, pick a single colony and transfer it to MRS liquid medium (containing 0.05g / L cysteine) for enrichment, and preserve it in 30% glycerol to obtain bacterial strain CCFM1147; among them, the typical colony of Lactobacillus paracasei It is milky white, irregular, round and convex, and smooth.

[0063] 2. Identification

[0064] The genome of bacterial ...

Embodiment 2

[0065] Example 2: Effect of Lactobacillus paracasei on the proliferation of psoriasis-like cortex-forming cells

[0066] Specific steps are as follows:

[0067] After rewarming the frozen HaCaT cells at 37°C, centrifuge at 1000rpm for 5min to take the pellet; wash the pellet with DMEM containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL streptomycin After the medium was washed once, the cells were resuspended in DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin and counted to obtain a cell resuspension; Inoculate the resuspension of cells into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were subcultured ...

Embodiment 3

[0071] Example 3: Effect of Lactobacillus paracasei on psoriasis-like cortex-forming cells NF-κB nuclear translocation

[0072] Specific steps are as follows:

[0073] After rewarming the frozen HaCaT cells at 37°C, centrifuge at 1000rpm for 5min to take the pellet; wash the pellet with DMEM containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL streptomycin After the medium was washed once, the cells were resuspended in DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin and counted to obtain a cell resuspension; Inoculate the resuspension of cells into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were subcu...

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PUM

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Abstract

The invention discloses lactobacillus paracasei capable of inhibiting abnormal proliferation of HaCaT cells, and belongs to the technical field of microorganisms and medicines. The invention providesa lactobacillus paracasei CCFM1147, and the lactobacillus paracasei CCFM1147 can alleviate psoriasis, which is specifically embodied in that the excessive proliferation of psoriasis-like cortical forming cells is inhibited; the activation of an NF-kB signal path in psoriasis-like cortical forming cells is inhibited; the level of proinflammatory factor IL-6 in psoriasis-like cortical forming cellsis reduced; the level of proinflammatory factor IL-8 in psoriasis-like cortical forming cells is reduced; and therefore, the lactobacillus paracasei CCFM1147 has a huge application prospect in the preparation of products (such as daily chemical products or medicines) for preventing and / or treating psoriasis.

Description

technical field [0001] The invention relates to a strain of Lactobacillus paracasei capable of inhibiting the abnormal proliferation of HaCaT cells, and belongs to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Psoriasis is a chronic inflammatory skin disease that is mediated by genetics, environment, and immunity. Next, autoimmune diseases mediated by keratinocytes and the immune system (especially T cells). More than 125 million people worldwide suffer from psoriasis, and the prevalence of psoriasis is increasing year by year. The global prevalence of psoriasis released by the World Health Organization in 2016 is 0.09-11.43%. Although psoriasis can occur at any age, the incidence is highest between the ages of 18-39 or 50-69. Psoriasis will not be life-threatening, but will seriously affect the health of the body. However, many papules and erythema of different sizes will appear on the upper layer of the skin, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61K9/06A61K9/70A61P17/06A61K8/99A61Q19/00A61Q19/10C12R1/245
CPCA61K35/747A61K9/0014A61K9/06A61K9/7007A61K47/14A61P17/06A61K8/99A61Q19/00A61Q19/10A61K2800/85C12R2001/245C12N1/205
Inventor 陈卫陆文伟邓雅丹翟齐啸杨波崔树茂张秋香赵建新张灏
Owner JIANGNAN UNIV
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