Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A bifidobacterium animal strain that can relieve psoriasis and its application

A technology of animal bifidobacteria and microbial strains, applied in the field of microorganisms, can solve problems such as obvious adverse reactions

Active Publication Date: 2021-12-03
JIANGNAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, it may be possible to start with lactic acid bacteria, by constructing a psoriasis-like pathological model, and looking for lactic acid bacteria that can effectively alleviate the condition of psoriasis, so as to find new drugs or new methods for the treatment of psoriasis, so as to overcome the existing therapeutic drugs and methods. Defects such as obvious adverse reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A bifidobacterium animal strain that can relieve psoriasis and its application
  • A bifidobacterium animal strain that can relieve psoriasis and its application
  • A bifidobacterium animal strain that can relieve psoriasis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: the acquisition of animal bifidobacterium

[0061] Specific steps are as follows:

[0062] 1. Screening

[0063] Taking human feces from Wuxi, Jiangsu as samples, the samples were pretreated and stored in 20% glycerin in a -80°C refrigerator. After taking out and thawing, mix the samples and add 0.5mL samples to 4.5mL to contain 0.05 Gradual dilution of 9 g / L normal saline with g / L cysteine, select the appropriate gradient dilution to spread on the MRS solid medium, culture at 37°C for 48 hours, pick typical colonies of Bifidobacterium animalis to the MRS solid Purify by streaking on the medium, pick a single colony and transfer it to MRS liquid medium (containing 0.05g / L cysteine) for enrichment, and preserve it in 30% glycerol to obtain strain CCFM1148; among them, the typical colony of Bifidobacterium animalis It is milky white, irregular, round and convex, and smooth.

[0064] 2. Identification

[0065] The genome of bacterial strain CCFM1148 was e...

Embodiment 2

[0066] Example 2: Effect of Bifidobacterium animalis on proliferation of psoriasis-like cortex-forming cells

[0067] Specific steps are as follows:

[0068] After rewarming the frozen human immortalized cortex-forming cells at 37°C, centrifuge at 1000rpm for 5min, and take the pellet; the pellet was washed with 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL chain After washing once with DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin, the cells were resuspended and counted to obtain cell weight. Suspension: Inoculate the cell resuspension into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were subcultured wh...

Embodiment 3

[0072] Example 3: Effect of Bifidobacterium animalis on NF-κB nuclear translocation in psoriasiform cortex-forming cells

[0073] Specific steps are as follows:

[0074] After rewarming the frozen human immortalized cortex-forming cells at 37°C, centrifuge at 1000rpm for 5min, and take the pellet; the pellet was washed with 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, 100mg / mL chain After washing once with DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin, the cells were resuspended and counted to obtain cell weight. Suspension: Inoculate the cell resuspension into a 10cm culture dish at 37°C with 5% (v / v) CO in the gas phase 2 cultured in a cell culture box, and the next day, the DMEM medium containing 5% (v / v) fetal bovine serum (FBS), 100U / mL penicillin, and 100mg / mL streptomycin was replaced once and continued at 37°C with 5% in the gas phase. (v / v)CO 2 cultured in a cell culture incubator. Cells were su...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an animal bifidobacterium capable of alleviating psoriasis and its application, belonging to the technical field of microorganisms and the technical field of medicine. The present invention provides a strain of animal bifidobacterium (Bifidobacterium animalis) CCFM1148, the animal bifidobacterium CCFM1148 can alleviate psoriasis, specifically embodied in: inhibiting the excessive proliferation of psoriasis-like cortex-forming cells; inhibiting the psoriasis-like cortex Activation of the NF‑kB signaling pathway in developing cells; reduced levels of the pro-inflammatory factor IL‑6 in psoriatic cortex-forming cells; reduced levels of the pro-inflammatory factor IL‑8 in psoriatic cortex-forming cells, visible, animals Bifidobacterium animalis CCFM1148 has great application prospects in the preparation of products for the prevention and / or treatment of psoriasis (such as daily chemical products or medicines, etc.).

Description

technical field [0001] The invention relates to an animal bifidobacterium capable of alleviating psoriasis and its application, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Psoriasis is a chronic, genetically and environmentally induced, immune-mediated inflammatory disease characterized clinically by scaly erythema or plaques, localized or widespread. Psoriasis is non-infectious and easy to relapse. It may gradually worsen with age, or the severity may fluctuate. More than 125 million people worldwide suffer from psoriasis, and the prevalence rate is increasing year by year. The global prevalence rate of psoriasis released by the World Health Organization in 2016 is 0.09-11.43%. Although psoriasis can occur at any age, the incidence rate is highest between the ages of 18-39 or 50-69. Although psoriasis has traditionally been considered limited to the skin and joints, in recent years there has been in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P17/06A61K8/99A61Q19/10A61Q19/00C12R1/01
CPCA61K8/99A61K35/745A61Q19/00A61Q19/10A61P17/06C12N1/205C12R2001/01
Inventor 陆文伟陈卫邓雅丹田丰伟翟齐啸王刚崔树茂赵建新张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com