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Bifidobacterium adolescentis capable of relieving psoriasis and application thereof

A technology for bifidobacteria and psoriasis, applied in the field of microorganisms, can solve problems such as unexplained skin relief effects

Active Publication Date: 2021-05-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the strain only alleviated the level of inflammation in the patient's serum, and did not describe the relief effect on the skin

Method used

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  • Bifidobacterium adolescentis capable of relieving psoriasis and application thereof
  • Bifidobacterium adolescentis capable of relieving psoriasis and application thereof
  • Bifidobacterium adolescentis capable of relieving psoriasis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Screening and identification of Bifidobacterium adolescentis

[0053] Specific steps are as follows:

[0054] Take human feces as the sample, after pretreatment, store the sample in 20% glycerol in a -80°C refrigerator, take it out and thaw it, mix the sample and add 0.5mL sample to 4.5mL to contain 0.05g / L half Cystine 9g / L physiological saline was used for gradient dilution, and the appropriate gradient dilution was selected and spread on the MRS solid medium, cultured at 37°C for 48h, and the typical colonies of Lactobacillus paracasei were picked and drawn on the MRS solid medium. Line purification, pick single bacterium colony and transfer to MRS liquid medium (containing 0.05g / L cysteine) enrichment, 30% glycerol preserves, obtains bacterial strain CCFM667; Wherein, the typical bacterium colony of Lactobacillus paracasei is milky white, not Regular, round convex, smooth.

[0055] The genome of CCFM667 was extracted, the 16S rDNA of CCFM667 was amplifi...

Embodiment 2

[0056] Embodiment 2: the cultivation of bifidobacterium adolescent

[0057] Specific steps are as follows:

[0058] After inoculating Bifidobacterium adolescentis CCFM667 into mMRS solid medium and culturing at 37°C for 48 hours, the colonies were observed and observed under a microscope, and it was found that the colonies were milky white, irregular, round and convex Rigid, smooth, slightly irregular-shaped, round-ended campylobacters, usually present singly, in pairs, and in small clusters.

[0059] Insert Bifidobacterium adolescentis CCFM667 into MRS liquid medium and culture it at 37°C for 30 hours, then transfer it to fresh MRS liquid medium, cultivate it under the same conditions for 30 hours, centrifuge the cells at 6000 g for 15 minutes, wash with 0.9% saline After the bacterial cells were centrifuged again at 6000 g for 10 min, the bacterial cells were obtained, and resuspended with 30% sucrose solution to obtain the bacterial sucrose suspension, which was ready for ...

Embodiment 3

[0060] Example 3: Relief effect of Bifidobacterium adolescentis on lesion skin of psoriasis mice

[0061] The SPF level BALB / c female mouse of 6-8 week age is divided into 4 groups, is respectively normal group, model group and experimental group, wherein, experimental group 1 is fed Bifidobacterium adolescentis CCFM667 (hereinafter abbreviated as CCFM667 group), Experimental group 2 was fed with Bifidobacterium adolescentis B.adolescentis 1 (hereinafter abbreviated as B.adolescentis 1 group), 6 animals in each group, and they were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, with a constant temperature of 21-26°C and a humidity of 40°C. -70%, noise less than or equal to 60dB, animal illumination 15-20LX (all animal experiment procedures are reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0062] The experimental period was 3 weeks in total, and the modeling began in the third week....

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PUM

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Abstract

The invention discloses bifidobacterium adolescentis capable of relieving psoriasis and application thereof, and belongs to the technical field of microorganisms and the technical field of medicine. The bifidobacterium adolescentis CCFM667 provided by the invention has the effect of relieving psoriasis, the condition of lesion skin of a psoriasis-like mouse is relieved, the thickening of the epidermal layer of the psoriasis-like mouse is inhibited, the spleen weight of the psoriasis-like mouse is reduced, the levels of IL-22 and IL-23 in the skin of the psoriasis-like mouse are reduced by 30.2%, and the bifidobacterium adolescentis has a huge application prospect in preparation of products (such as foods, medicines or health foods) for preventing and / or treating psoriasis.

Description

technical field [0001] The invention relates to a bifidobacterium adolescent capable of relieving psoriasis and application thereof, and belongs to the technical field of microbes and the technical field of medicine. Background technique [0002] Psoriasis is a chronic, immune-mediated disease, mainly affecting the skin and joints, with complex heredity, with a global prevalence of about 2-3%, and the prevalence is increasing year by year. Although it can occur at any age, the incidence is highest between the ages of 18-39 or 50-69. As a skin disease, psoriasis can manifest in a variety of phenotypes, including plaque psoriasis (also known as psoriasis vulgaris), punctate psoriasis, inverse psoriasis, pustular psoriasis psoriasis, palmoplantar psoriasis, and erythrodermic psoriasis. Multiple phenotypes can occur in the same individual, of which psoriasis vulgaris is the most common. Symptoms common to all phenotypes include pruritus, burning, and soreness. Psoriasis is n...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L29/00A23L11/50A23L19/00A23C9/123A61K35/745A61P17/06C12R1/01
CPCC12N1/20A23L29/065A23L11/00A23L19/00A23C9/1234A61K35/745A61P17/06A23V2400/513
Inventor 陆文伟陈卫邓雅丹翟齐啸崔树茂王刚张秋香赵建新张灏
Owner JIANGNAN UNIV
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