In-vitro drug sensitivity detection method based on drug probe and tissue slice

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to an in-vitro drug sensitivity detection method based on a drug probe and a tissue slice. According to the invention, a drug probe in chemical biology is coupled with fluorescein by utilizing click chemistry, so that drug visualization is realized. The method does not depend on primary culture or animalmodels, and is short in detection period, high in success rate and free of secondary trauma; the method has very wide clinical application and scientific research value, and can be particularly usedfor predicting the drug effect of the anti-tumor targeted drug so as to guide a patient to take the drug; the method also has important guiding significance for patient grouping in a new drug clinicaltest; in addition, staining results of some targeting drug probes of the method can also be used for auxiliary pathological diagnosis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to an in vitro drug sensitivity detection method based on drug probes and tissue slices. Background technique [0002] Small molecule drugs (including chemotherapy drugs and targeted drugs) play an important role in the treatment of malignant tumors and are commonly used in clinical treatment. However, malignant tumors are heterogeneous. Even the same tumor with the same pathological type and histological type has different sensitivities to the same small molecule drug. Multidrug resistance is common, and there are large individual differences among patients. Factors may lead to treatment failure. Moreover, there are many kinds of chemotherapeutic drugs and targeted drugs currently on the market, and choosing a treatment plan based on clinical experience alone has a certain degree of blindness, low therapeutic efficiency, high toxicity and side effects, and easy drug resistance...

AI Technical Summary

Problems solved by technology

The success rate of primary culture of tumor cells is low, the culture period is long, and the efficiency is low, which seriously limits the use of this method

In addition, the traditional cell viability detection method also has many shortcomings, such as tumor cell colony assay, the primary culture period is long, the specimen evaluation rate is low (40%-70%), the operation is cumbersome, and it is difficult to standardize; Based on azolium salt) colorimetric method, the number of cells required is large, the experimental data is unstable, and the detected cells are living cells, which cannot distinguish mesenchymal cells from tumor cells, so when applied to highly heterogeneous clinical tumor tissues It is difficult to accurately reflect its drug sensitivity

PDX requires high cost, long cycle, and certain limitations

The current gene sequencing used to guide medication can only give information on gene mutations, not directly detect drug targets, so the accuracy is low

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