Strawberry vitamin C synthesis related gene FaGalLDH and application

Abstract

The invention discloses a strawberry vitamin C synthesis related gene FaGalLDH and application. The strawberry vitamin C synthesis related gene FaGalLDH has a nucleotide sequence shown as SEQ ID NO: 1. The invention further discloses application of the strawberry vitamin C synthesis related gene FaGalLDH in increasing the content of vitamin C and application of the strawberry vitamin C synthesis related gene FaGalLDH in improving the salt tolerance of plants. After the gene is over-expressed in arabidopsis thaliana, a transgenic plant with the content of vitamin C (AsA) remarkably increased isobtained, and the salt tolerance of the transgenic plant is improved. Experiments prove that the FaGalLDH gene can synthesize vitamin C in vitro, the optimal induction condition is found through optimization, the content of vitamin C in transgenic arabidopsis thaliana leaves can be remarkably increased through over-expression, and no obvious influence is caused to normal growth of plants. Proteinand an encoding gene thereof have important theoretical and practical significance in research of a plant vitamin C synthesis mechanism and improvement and stress resistance of the content of vitaminC of plants, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of plant molecular genetics and genetic engineering, in particular to a gene FaGalLDH related to the synthesis of strawberry vitamin C and its application. Background technique [0002] Strawberry (Fragaria×ananassa Duch.) is a perennial herb of the Rosaceae Fragaria genus. The fruit is bright in color, rich in aroma, sweet and sour, and has high nutritional and health value. It has the reputation of "Queen of Fruits". Strawberry fruit has been shown to have strong antioxidant activity associated with the vitamin C and polyphenolic compounds contained in the fruit. Vitamin C (also known as ascorbic acid) is a water-soluble vitamin, which not only has a positive preventive effect on human diseases such as scurvy, arteriosclerosis, and hypertension, but also plays an anti-oxidant, antioxidant role in the growth and development of plants. stimulating and other vital roles. In addition, vitamin C is also one o...

AI Technical Summary

Problems solved by technology

L-galactose-1,4-lactone dehydrogenase (GalLDH) is a key enzyme in the synthesis of vitamin C, but there are few reports on the GalLDH gene in strawberry fruit, so the role of GalLDH gene in vitamin C accumulation is clarified. mechanism, critical to strawberry production

Images