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Selenium-enriched probiotics for affecting mouse diarrhea and intestinal canal florae caused by irinotecan

A technology of selenium-enriched probiotics and irinotecan, which is applied in the field of microorganisms and can solve problems such as impact

Pending Publication Date: 2020-12-15
JIANGSU DAYSEBIOTECH LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the adverse reactions of the current clinical application of CPT-11, the present invention provides a probiotic that affects mouse diarrhea and intestinal flora caused by irinotecan

Method used

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  • Selenium-enriched probiotics for affecting mouse diarrhea and intestinal canal florae caused by irinotecan
  • Selenium-enriched probiotics for affecting mouse diarrhea and intestinal canal florae caused by irinotecan
  • Selenium-enriched probiotics for affecting mouse diarrhea and intestinal canal florae caused by irinotecan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 Se-DD98

[0044] The Bifidobacterium longum DD98 strain (Bifidobacterium.longum DD98) used in the present invention, after extracting the bacterial genome DNA, is amplified using the 16S universal primer of the bacterium, and the 16SrDNA gene sequence of the bacterium is amplified, and the sequence is as shown in SEQ ID No: 1 shown.

[0045]By using 16SrDNA sequencing and BLAST analysis of nucleotide sequences, the strain is not completely identical to the corresponding sequences of all other Bifidobacterium longum known so far, but has certain homology. Through the analysis of sugar fermentation characteristics, compared with "Bergey's Bacteria Identification Manual", it is further confirmed that the strain belongs to Bifidobacterium longum, but it is different from the standard strain Bifidobacterium longum JCM 11342 (see Genebank number for its 16S rDNA sequence: LC306854. 1) The result of comparing the 16S rDNA sequence shows that th...

Embodiment 2

[0053] Example 2 Changes in body weight of mice

[0054] The mice were randomly divided into 4 groups, 12 in each group, which were normal control group, CPT-11 group, Se-DD98 low-dose group, and Se-DD98 high-dose group. The normal control group and the CPT-11 group were intragastrically administered distilled water, the Se-DD98 low-dose group was intragastrically administered bacterial powder (the number of viable bacteria was 1×108 CFU / kg), and the Se-DD98 high-dose group was intragastrically administered bacterial powder (the number of viable bacteria was 1× 109 CFU / kg), 0.2 ml / 10g, 1 time / day. Establish irinotecan diarrhea model. From day 0 to day 24, the mice in each group were intraperitoneally injected with sterile water or bacterial solution; on day 18, the normal control group was intraperitoneally injected with sterile saline, and each mouse in the other groups was intraperitoneally injected with 150 mg / kg CPT- 11. The injection volume is 0.1 ml / 10 g; on the 17th d...

Embodiment 3

[0064] Example 3 Incidence of diarrhea in mice and evaluation of diarrhea grade

[0065] After 24 hours of irinotecan injection, the mice in each group were observed for diarrhea and scored at 48, 72, 96, 120 and 144 hours, respectively.

[0066] Table 4 Evaluation criteria for diarrhea in mice

[0067]

[0068] Table 5 Effect of Se-DD98 on the average diarrhea grade of mice with irinotecan-induced diarrhea

[0069]

[0070] Table Note: Compared with the normal group, *p△ p△△ p<0.01.

[0071] in conclusion:

[0072] 1. figure 2 The results showed that the incidence of diarrhea in the irinotecan group and the Se-DD98 low-dose group changed over time, showing a trend of increasing gradually at first, then decreasing and then remaining unchanged. The Se-DD98 high-dose group remained unchanged after 48 hours of decline, but the incidence of diarrhea in the Se-DD98 high-dose and low-dose groups was lower than that of the irinotecan group;

[0073] 2. The results in Tab...

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Abstract

The invention discloses selenium-enriched probiotics for affecting mouse diarrhea and intestinal canal florae caused by irinotecan. Mouse models suffering from diarrhea caused by the irinotecan are established, the selenium-enriched probiotics are administered through long-term intragastric administration, and influence on the mouse diarrhea and intestinal canal florae caused by the irinotecan caused by the selenium-enriched probiotics is evaluated. Mice of an experimental group are administered with the selenium-enriched probiotics every day through intragastric administration, and body weight, diarrhea levels, mouse immunity indexes, intestinal canal florae and ileum tissue structures of the mice are measured. A result shows that the selenium-enriched probiotics can inhibit loss of weight and lowering of immunologic functions caused by the irinotecan; the diarrhea incidence rate and average diarrhea level of the mice are lowered, and the mouse diarrhea caused by the irinotecan is relieved; the content of clostridium, escherichia coli and bacteroides related to beta-D-glucuronidase is lowered, and the content of bifidobacterium in an intestinal canal is increased; and the injury to intestinal canal mucosae, cells and glands of the mice induced by the irinotecan is relieved.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a selenium-enriched probiotic that affects mouse diarrhea and intestinal flora caused by irinotecan. Background technique [0002] Irinotecan (CPT-11) is a compound derived from camptothecin, which is widely used in the treatment of various tumor diseases, such as non-small cell lung cancer, gastric cancer and rectal cancer. After entering the body, CPT-11 can be rapidly hydrolyzed into the active compound SN-38 in the gastrointestinal tract and liver tissue, and SN-38 is metabolized into the inactive metabolite SN-38 glucuronide (SN-38G ), which are then exported to the gut. However, SN-38G can be hydrolyzed to SN-38 by intestinal bacterial β-D-glucuronidase, leading to enterotoxicity. CPT-11 can also cause adverse reactions such as myelosuppressive toxicity, neutropenia and leukopenia. [0003] Bifidobacteria are living intestinal microorganisms, as medicines or food supple...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61K35/745A61P1/00A61P1/12A61P39/02
CPCA61K35/741A61K35/745A61P1/00A61P1/12A61P39/02Y02A50/30
Inventor 祁艳陈代杰邵雷谭俊朱慧周艳
Owner JIANGSU DAYSEBIOTECH LTD
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