Method for screening pharmaceutically active compounds from traditional Chinese medicines and acting agent
A technology for screening drugs and drug effects, applied in the field of methods and agents, screening drug active compounds, can solve the problems of long time, high cost of experimental materials, small throughput, etc., and achieve the effect of high screening efficiency
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[0040] In order to enable those skilled in the art to better understand the technical solutions in the application, the present invention will be further described below in conjunction with the following examples. Obviously, the described embodiments are only a part of the embodiments of the application, rather than all Example. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the scope of protection of this application.
[0041] 1. High-throughput screening based on high-throughput sequencing
[0042] A high-throughput screening platform based on signature gene expression profiles can quantitatively analyze changes in transcriptional profiles in cells on a large scale. We examined the regulatory effects of 513 traditional Chinese medicine extracts on key signaling pathways of cytokine storm in immune cell THP1. These 513 kinds of traditional Chinese medicine...
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