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A kind of induction method of rubber grass tetraploid plant

A rubber grass, tetraploid technology, applied in the field of plant genetics and breeding, can solve the problems of rubber grass damage, low induction rate, etc., to achieve the effect of reducing chimerism, increasing mutation rate, and improving seedling rate

Active Publication Date: 2022-03-29
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] When inducing chromosome doubling, the more commonly used method is to use colchicine to treat rubber grass, but the present invention finds that the induction rate of this method is low, and it is very harmful to rubber grass

Method used

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  • A kind of induction method of rubber grass tetraploid plant
  • A kind of induction method of rubber grass tetraploid plant

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Effect test

Embodiment 1

[0025] The present embodiment provides a kind of rubber grass tetraploid plant induction method (process diagram sees figure 1 ), the method includes the following steps:

[0026] Step 1: Soak rubber grass seeds in 1% hydrogen peroxide and sterilize them for 1 hour, then rinse them with water, put them into a petri dish with filter paper to germinate, and when the seeds germinate and the radicle grows to about 5-10 mm, perform mutagenesis treatment;

[0027] Step 2: Soak the rubber grass seedlings in 0.04mM oryzalin solution for 30 minutes, then wash them with water for 4 times, and transplant them to culture soil (vermiculite + peat soil = 3:1) for cultivation. The cultivation temperature is 18 ~ 26°C, 16 hours of light (natural light + 50klux fluorescent light supplementary light);

[0028] Step 3: After the plant grows to 5-7 leaves, use flow cytometry or root tip cell chromosome pressing technology to screen and identify the tetraploid plant of rubber grass.

Embodiment 2

[0030] The present embodiment provides a kind of rubber grass tetraploid plant induction method, and the method comprises the following steps:

[0031] Step 1: Soak rubber grass seeds in 1% hydrogen peroxide and sterilize them for 1 hour, then rinse them with water, put them into a petri dish with filter paper to germinate, and when the seeds germinate and the radicle grows to about 5-10 mm, perform mutagenesis treatment;

[0032] Step 2: Immerse the rubber grass seedlings in 0.04mM oryzalin solution for 1 hour, then wash them with water for 4 times, and transplant them to culture soil (vermiculite + peat soil = 3:1) for cultivation. The cultivation temperature is 18 ~ 26°C, 16 hours of light (natural light + 50klux fluorescent light supplementary light);

[0033] Step 3: After the plant grows to 5-7 leaves, use flow cytometry or root tip cell chromosome pressing technology to screen and identify the tetraploid plant of rubber grass.

Embodiment 3

[0035] The present embodiment provides a kind of rubber grass tetraploid plant induction method, and the method comprises the following steps:

[0036] Step 1: Soak rubber grass seeds in 1% hydrogen peroxide and sterilize them for 1 hour, then rinse them with water, put them into a petri dish with filter paper to germinate, and when the seeds germinate and the radicle grows to about 5-10 mm, perform mutagenesis treatment;

[0037] Step 2: Soak the rubber grass seedlings in 0.08mM oryzalin solution for 30 minutes, then wash them with water for 4 times, and transplant them to culture soil (vermiculite + peat soil = 3:1) for cultivation. The cultivation temperature is 18 ~ 26°C, 16 hours of light (natural light + 50klux fluorescent light supplementary light);

[0038] Step 3: After the plant grows to 5-7 leaves, use flow cytometry or root tip cell chromosome pressing technology to screen and identify the tetraploid plant of rubber grass.

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Abstract

The invention relates to the field of plant genetics and breeding, in particular to a method for inducing tetraploid plants of rubber grass. During mutagenesis treatment, the rubber grass seedlings are soaked in 0.04-0.08 mM asulphin solution for 30-60 minutes. Through the optimization of the method for inducing the tetraploid plant of the rubber grass, the present invention greatly improves the mutagenesis rate, reduces the chimera, and significantly improves the seedling rate at the same time. During application, the mutation rate of tetraploid plants can reach more than 10%, and the seedling rate can reach more than 70%, forming a set of efficient and stable induction methods for tetraploid plants of rubber grass.

Description

technical field [0001] The invention relates to the field of plant genetics and breeding, in particular to a method for inducing tetraploid plants of rubber grass. Background technique [0002] Rubber grass, also known as Russian dandelion, is a perennial herb of the genus Dandelion in the family Asteraceae. As a potential alternative plant for rubber production, rubber grass has attracted widespread attention at home and abroad in recent years. Wild rubber grass contains 4-5% high-quality natural rubber, which mainly exists in the root of rubber grass. Polyploid plants bred by polyploid breeding often have the characteristics of large plant organs, high yield, strong stress resistance, and robust growth, and are one of the important methods in plant breeding. Rubber grass seeds are very small, with a length of only about 0.5cm and a thousand-grain weight of only about 0.5g. [0003] When inducing chromosome doubling, the more commonly used method is to treat the rubber g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 杨玉双覃碧王若旭刘实忠王学卓张继川张立群
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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