Sodium ion corrected erythrocyte hematocrit test system and test method

Abstract

The invention discloses a sodium ion corrected erythrocyte hematocrit test system and a test method, and the system comprises a sodium ion electrode test unit for measuring the concentration of sodiumions in a blood sample; an impedance test unit for measuring the whole blood impedance of the blood sample; and a calculating unit which is connected with the sodium ion electrode test unit and the impedance test unit and is used for calculating the hematocrit of the blood sample. According to the system, the sodium ion electrode test unit is added to carry out a sodium ion concentration test ina whole blood hematocrit test of an impedance electrode, and the influence of the factor on whole blood impedance is corrected, so that the accuracy of the hematocrit test is improved, and clinical errors are avoided. Meanwhile, the test system is simple in structure, high in test speed, convenient to use, low in price and low in HCT detection cost. The test method is small in error and high in test efficiency and accuracy.

Description

technical field [0001] The invention belongs to the technical field of biological analysis instruments, and relates to a hematocrit test system and a test method, in particular to a sodium ion-corrected impedance method hematocrit test system and a test method. Background technique [0002] Hematocrit, also known as hematocrit (HCT), refers to the volume ratio of the sinking red blood cells to the whole blood measured after a certain amount of anticoagulated whole blood is centrifuged. It is a measure that indirectly reflects the number and volume of red blood cells. easy way. Combined with red blood cell count and hemoglobin content, the average value of red blood cells can be calculated, which is helpful for the morphological classification of anemia, and is of great significance for the diagnosis of anemia, rehydration of dehydrated patients, and blood rheology. [0003] At present, HCT determination methods mainly include Wen's centrifugation method, blood cell analyzer...

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Problems solved by technology

The above-mentioned method has the following technical problems: (1) Wen's centrifugation method is a classic definition method, but it cannot completely eliminate the influence of residual plasma, and the accuracy is low, and this method cannot be conveniently combined with other methods, and the amount of sample used (2) The blood cell analyzer method calculates hematocrit by measuring red blood cells, white blood cells, etc., which is mainly calculated by empirical formulas, so that biological variation cannot be measured. In addition, this method needs to use a blood cell analyzer to input Calculated value, it is inconvenient to test HCT alone clinically; (3) The indirect empirical calculation method of hemoglobin cell derivatives measures hemoglobin through spectroscopy, and then calculates HCT through empirical formulas. In clinical practice, expensive spectral equipment must be installed, which is inconvenient for one-time use, and the cost is high (4) The direct electrical impedance method is to obtain the hematocrit value by the impedance value by measuring whole blood red blood cells, but the above method is calculated by the red blood cell impedance law under the usual electrolyte level, and in the case of electrolyte disturbance, it is prone to errors or even unrecognizable

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