A production method of nanoporous glass for oligonucleotide synthesis

A technology of borosilicate glass and acid solution, which is applied in the field of material processing, can solve the problems of high production cost, uneven channel, environmental pollution and other problems in the production process, and achieve the effects of reducing output ratio, simple operation and simple process

Active Publication Date: 2021-10-08
河北迪纳兴科生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, the CPG products produced by the existing technology still have uneven channels and clogging, and the production process has problems such as high production cost, low efficiency and serious environmental pollution.

Method used

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  • A production method of nanoporous glass for oligonucleotide synthesis
  • A production method of nanoporous glass for oligonucleotide synthesis
  • A production method of nanoporous glass for oligonucleotide synthesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1: a kind of method of producing CPG

[0081] 1000g of borosilicate glass (80-100 mesh) is placed in a 1L spherical reactor, using a 200-mesh screen, 3L of 1.5mol / L nitric acid is injected into 1L from the top of the spherical reactor through the acid tank through the circulation pump, and the injection is completed Start the oil bath and heat it to 80°C, start the stirring system and adjust the speed to 300 rpm, and stir for 3 hours. This stirring process mainly makes the sodium boron in the borosilicate glass dissolve in the acid. Stop stirring, after the acid liquid in the still is discharged from the waste liquid port, start the circulation pump, the pressure of the circulation pump is 0.5Mpa, pass the acid liquid in the acid liquid tank into the reaction kettle, and circulate for 3 hours, close the acid liquid valve, and put the acid liquid in the still Put the acid solution into the acid solution tank, then open the tank valve, the volume of the tank is...

Embodiment 2

[0083] Embodiment 2: a kind of method of producing CPG

[0084] 5000g of borosilicate glass (80-100 mesh) is placed in a 5L spherical reactor, using a 200-mesh screen, 15L of 0.75mol / L sulfuric acid is injected into 5L from the top of the spherical reactor through the acid tank through the circulation pump, and the injection is complete. Start the oil bath and heat it to 85°C, start the stirring system and adjust the speed to 300 rpm, and stir for 3 hours. This stirring process mainly makes the sodium boron phase in the borosilicate glass dissolve in the acid. Stop stirring, and the acid solution in the kettle After being discharged from the waste liquid port, start the circulation pump, the pressure of the circulation pump is 0.5Mpa, pass the acid solution in the acid solution tank into the reaction kettle, circulate for 3 hours, close the acid solution valve, and pour the remaining acid solution in the tank into the acid solution tank , open the water tank valve, the volume ...

Embodiment 3

[0086] Embodiment 3: a kind of method of producing CPG

[0087]20,000g of borosilicate glass (80-100 mesh) is placed in a 20L spherical reactor, using a 200-mesh screen, 60L of 1.5mol / L hydrochloric acid is injected from the top of the spherical reactor through the acid tank through the circulation pump, and 20L is completed Start the oil bath and heat it to 98°C, start the stirring system and adjust the rotation speed to 300 rpm, and stir for 3 hours. This stirring process mainly makes the sodium boron phase in the borosilicate glass dissolve in the acid. Stop stirring and dissolve the acid solution in the kettle. After being discharged from the waste liquid port, start the circulation pump, the pressure of the circulation pump is 0.5Mpa, pass the acid solution in the acid solution tank into the reaction kettle, circulate for 3 hours, close the acid inlet valve, and pour the remaining acid solution in the tank into the acid solution tank , open the water tank valve, the volum...

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Abstract

The invention belongs to the technical field of material processing, and in particular relates to a method for producing nanoporous glass with controllable pore size by a cycle integration device. The present invention adopts an integrated device for CPG production, and sets different processing conditions for borosilicate glass with different meshes, which can minimize waste liquid output, reagent consumption, and processing time, greatly reduce processing costs, and improve production efficiency , reduce energy consumption, and obtain products with more uniform pores and no clogging phenomenon, which will play a powerful role in promoting the domestic gene synthesis industry, oligonucleotide drug research and other fields.

Description

Technical field: [0001] The invention belongs to the technical field of material processing, and in particular relates to a method for producing nanoporous glass with controllable pore size by a cycle integration device. Background technique: [0002] Controlled pore glass (Controlled pore glass) is a carrier material for the synthesis of oligonucleotides, referred to as CPG. Oligonucleotides are fundamental tools for modulating gene expression in biomedical and life science research, and can be developed as gene-targeted therapeutics, which are then used to treat viruses, tumors, and genetic diseases. The 21st century is the century of life science and biotechnology. Domestic biotechnology research has developed rapidly, among which biotechnology such as gene synthesis technology, amplification technology, gene modification technology, gene cloning technology, gene chip technology, and gene therapy technology have already been recognized. Included in the "China High-Tech D...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C03C15/00
CPCC03C15/00
Inventor 卢晋华张阳
Owner 河北迪纳兴科生物科技有限公司
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