Micro-fluidic chip and method for sorting micro-nano particles through inertia turbulent flow

Abstract

The invention discloses a micro-fluidic chip and a method for sorting micro-nano particles by inertia turbulent flow. The micro-fluidic chip comprises an upper-layer substrate and a lower-layer substrate, wherein the upper-layer substrate and the lower-layer substrate are bonded together in a sealing manner to form the micro-fluidic chip; the upper-layer substrate is provided with a liquid inlet hole, an inlet liquid storage tank, an inertia flow channel, a sudden expansion flow channel, a turbulent flow barrier, an inner outlet flow channel, an inner outlet liquid storage tank, an inner liquid outlet hole, an outer outlet flow channel, an outer outlet liquid storage tank and an outer liquid outlet hole; the liquid inlet hole is sequentially communicated with the inlet liquid storage tank,the inertia flow channel and the sudden expansion flow channel; then the liquid is divided into two branches, one branch is sequentially communicated with the inner outlet flow channel, the inner outlet liquid storage pool and the inner liquid outlet hole, and the other branch is sequentially communicated with the outer outlet flow channel, the outer outlet liquid storage pool and the outer liquid outlet hole; and the turbulent flow barrier is positioned in the sudden expansion flow channel. The micro-fluidic chip overcomes the technical bottleneck that the traditional inertial micro-fluidicchip is difficult to accurately sort micro-nano particles, and has the advantages of simple structure, good control precision and high flux.

Description

technical field [0001] The invention relates to a microfluidic chip and method for sorting micro-nano particles by inertial disturbance, which belongs to the field of microfluidics and can be used for precise manipulation of micro-nano biological particles and cells, such as precise capture and focusing of micro-nano biological cells and sorting etc. Background technique [0002] Portable real-time detection instruments have important application value in dealing with sudden on-site acute disease diagnosis, early screening and prognosis assessment of malignant diseases, and promoting the development of personalized medicine. highly valued. As the key technology of real-time detection instruments, microfluidic chips have the advantages of fast detection speed, high sensitivity, low cost, and good integration. They are very suitable for the technical needs of real-time detection and have become a research hotspot in this field. [0003] Precise manipulation of biological cel...

AI Technical Summary

Problems solved by technology

However, the fluid inertia effect has a strong dependence on the apparent size of cells, and it is difficult to precisely manipulate cells with high concentrations and similar sizes (such as separating and capturing circulating tumor cells in blood), and the precise acquisition of such cells is crucial for some major diseases. Diagnosis, monitoring and treatment are of great practical and scientific value

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