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A microfluidic device and method for sorting droplets based on the principle of dielectrophoresis

A microfluidic device and a technology of dielectrophoresis, which are applied in the field of microfluidics, can solve the problems of inaccurate droplet sorting, and achieve the effects of simple structure and volume reduction.

Inactive Publication Date: 2021-04-30
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microfluidic devices for sorting droplets are not accurate enough in droplet sorting.

Method used

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  • A microfluidic device and method for sorting droplets based on the principle of dielectrophoresis
  • A microfluidic device and method for sorting droplets based on the principle of dielectrophoresis
  • A microfluidic device and method for sorting droplets based on the principle of dielectrophoresis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: as figure 1As shown, a microfluidic device for sorting droplets based on the principle of dielectrophoresis, including a glass substrate 1 and a PDMS chip 2 fixed thereon, the PDMS chip 2 is provided with a primary sorter 9 and a secondary The first-stage sorter 7, the first-stage sorter 9 and the second-stage sorter 7 have the same structure, both of which are composed of a transverse channel that can pass through a single droplet and two branch channels that are connected to the transverse channel. A sorting structure and a balance pressure auxiliary structure are provided between the branch channels, a first electric field 4 is provided on the transverse channel of the primary separator 9, a second electric field 5 is provided on the transverse channel of the secondary separator 7, and a second electric field 5 is provided on the transverse channel of the secondary separator 7. Both the first electric field 4 and the second electric field 5 are composed...

Embodiment 2

[0039] Embodiment 2: In this embodiment, the structure of the first-level sorter 9 and the second-level sorter 7 is described in detail. Because the two structures are the same, the first-level sorter 9 is used for illustration here, specifically as Figure 2-3 As shown, the first-stage sorter 9 includes a lateral channel composed of a limiting channel 9-1 and an electric field microchannel 9-2, a split inlet 9-3, a first sorting structure 9-5, and a second sub-structure 9-9. The sorting structure, the balance pressure auxiliary structure composed of the first auxiliary structure 9-6, the second auxiliary structure 9-7 and the third auxiliary structure 9-8.

[0040] One end of the limiting channel 9-1 is connected to the inlet 3, and the other end is connected to the electric field microchannel 9-2. The limiting channel 9-1 mainly allows liquid droplets to pass through individually. The upper pole plate and the lower pole plate of the electric field 4, the end of the electric ...

Embodiment 3

[0047] Embodiment 3: In this example, the specific dimensions of each part are limited, wherein the PDMS chip 2 is 120mm long, 60mm wide, and 10mm high, and the angle between the two branch channels of the primary sorter 9 is 30° ,like image 3 shown. The width of the limiting passage 9-1 is 0.025mm, the height is 0.025mm, and the length is 3mm; the length of the first sub-channel 9-4 is 0.050mm, and the width is 0.025mm; the length of the second sub-channel 9-10 is 0.100mm, the width is 0.025mm; the width of the microchannel between the first sorting structure 9-5 and the second sorting structure 9-9 is 0.010mm, the first auxiliary structure 9-6, the second auxiliary structure 9 The distance between -7 and the third auxiliary structure 9-8 is 0.015mm, and the widths of the three auxiliary structures are all 0.045mm. Other connecting channels have a width of 0.050mm and a height of 0.050mm.

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Abstract

The invention relates to a microfluidic device and method for sorting droplets based on the principle of dielectrophoresis, and belongs to the technical field of microfluidics. The invention includes a glass substrate and a PDMS chip fixed thereon, a microchannel structure made by a microfabrication technology, and a microchannel inside a sorter. The invention allows droplets to pass through a microchannel with an electric field, and under the action of dielectrophoretic force, the droplets wrapping a single cell gather at the lower part of the channel, and the droplets move forward continuously in the continuous phase fluid to reach the special When the position of the sorting structure is different, under the different resistance of the two sub-channels, the droplet wrapping a single cell enters the lower sub-channel, and the droplet wrapping two or more cells enters the upper channel, so as to achieve the separation and packaging The effect of a single cell droplet, after the droplet enters the sub-channel, under the action of the balanced pressure auxiliary structure at the back, the droplet moves forward at a uniform speed, avoiding backflow, and realizing the precise sorting of the droplet.

Description

technical field [0001] The invention relates to a microfluidic device and method for sorting droplets based on the principle of dielectrophoresis, and belongs to the technical field of microfluidics. Background technique [0002] Microfluidic chips are widely used in disease diagnosis, drug sorting, environmental testing, food safety and other fields. Droplet microfluidic technology is to generate and manipulate droplets in a closed micro-scale channel. It is a simple structure and high-throughput biochemical reagent reaction method. Droplet microfluidics means that there are two immiscible liquids in the microfluidic chip, such as water phase and oil phase, which are endowed with two fluids of dispersed phase and continuous phase by means of droplet generation. Unit nanoliter to picoliter droplets are dispersed in the latter. As a new form of fluid motion that has emerged in the field of microfluidic chips in recent years, liquid droplets have become an effective means to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N15/10
CPCB01L3/502753G01N15/10G01N2015/1081
Inventor 龙威刘云赵娜陈娅君魏先杰吴蜜蜜王继尧
Owner KUNMING UNIV OF SCI & TECH
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