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Targeted chitosan nano-silver gel as well as preparation method and application thereof

A chitosan nano, silver gel technology, applied in pharmaceutical formulations, medical preparations with inactive ingredients, medical preparations containing active ingredients, etc., to achieve broad application prospects, avoid drug resistance, dispersibility and stability good effect

Active Publication Date: 2020-12-01
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome the defects of existing nano-silver reagents, and provide materials containing imidazole cross-linked chitosan, in-situ complexation reduction nano-silver structure and hyaluronic acid targeting structure and its preparation method

Method used

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  • Targeted chitosan nano-silver gel as well as preparation method and application thereof
  • Targeted chitosan nano-silver gel as well as preparation method and application thereof
  • Targeted chitosan nano-silver gel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Put 0.5g of dimethyl imidazole-4,5-dicarboxylate into a 100mL three-neck round bottom flask, add 10mL of methanol, and heat to reflux at 70°C until the dimethyl imidazole-4,5-dicarboxylate is completely dissolved Afterwards, 2 mL of 80% hydrazine hydrate was added and refluxed for 3 h, washed with methanol, centrifuged twice, and the obtained sample was vacuum-dried to obtain the product imidazole-4,5-dicarboxylic acid dimethylhydrazide. Such as figure 1 Shown is imidazole-4,5-dicarboxylic acid dicarboxylhydrazide 1 HNMR spectrum. Dimethyl imidazole-4,5-dicarboxylate ( figure 1 a): δ3.8 (CH3), δ7.9 (CH), δ13.5 (NH). Imidazole-4,5-dicarboxylic acid dicarboxylic acid hydrazide ( figure 1 b): After hydrazinolysis, the proton resonance peak of the methoxy group δ3.8 disappears, the new resonance peaks of δ9.7 and δ12.0 are attributed to the NH peak of the hydrazide group, and δ4.7 is assigned to the NH2 peak of the hydrazide group , indicating that the hydrazinolysis w...

Embodiment 2

[0040] Weigh 1.7760 g of chitosan (MW=5KDa), place it in a 250 mL three-necked round bottom flask, dissolve it in 50 mL of NaAc / HAc buffer solution with pH=4.5, and ultrasonicate for 30 min to dissolve it. Weigh 0.2246g of sodium periodate, dissolve it in 20mL of NaAc / HAc buffer solution with pH=4.5, place the two solutions in an ice bath, fill with nitrogen gas, degas for 20 minutes, mix them, and stir them in an ice bath for 24 hours Afterwards, 10 mL of ethylene glycol was added to terminate the reaction. Take out the product solution, pour it into a petri dish, put it into a refrigerator to freeze at -20°C, take out the solidified sample and place it in a freeze dryer to freeze-dry to obtain the product formyl chitosan.

[0041] Weigh 0.05g of formyl chitosan in a single-necked flask and add 20mL of deionized water to dissolve it, dissolve 0.0055g of imidazole-4,5-dicarboxylic acid diformylhydrazide with 10mL of dimethyl sulfoxide, and add formyl chitosan to the Add imidazo...

Embodiment 3

[0043] Weigh 15mg of formyl chitosan (MW=5kDa) in 30mL of deionized water, heat and ultrasonicate in a water bath at 70°C, until it is completely dissolved, slowly add 5mL of 0.6mg / mL imidazole-4,5-dicarboxylate dropwise Acid dimethyl hydrazide solution, after ultrasonication for 20 minutes, add 2 mL of 1.7 mg / mL silver nitrate solution dropwise to the reaction solution, continue ultrasonication for 10 minutes, adjust the pH value to 8-9 with sodium hydroxide solution, and add 10% boron dropwise Sodium hydride solution 0.5mL, then dropwise add 1% hyaluronic acid solution with a volume ratio of 1:1 to imidazole cross-linked chitosan gel (CFCS), and continue ultrasonication for 20min, dialyze the product solution (MWCO=2000kDa), freeze Dry to obtain targeting nano-silver gel (HCFCS@Ag). Such as image 3 Shown are the TEM images of the targeted chitosan nanogel (HCFCS) prepared in Example 2 and the targeted nanosilver gel (HCFCS@Ag) prepared in this example. The particle size d...

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Abstract

The invention discloses targeted chitosan nano-silver gel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the method,chitosan and imidazole-4,5-dicarboxylic acid dimethyl ester are used for preparing imidazole cross-linked chitosan, and hyaluronic acid is adopted for coating, so that the targeting property of the imidazole cross-linked chitosan is achieved; the cross-linked gel, silver nitrate and sodium borohydride are adopted for in-situ reduction synthesis of the targeted nano-silver gel. The prepared nano-silver is good in dispersity and stability, has an obvious inhibiting effect on fungi such as candida albicans, has an obvious inhibiting effect on tumor cells such as Hela, also has a potential inhibiting effect on bacteria and viruses, and has a wide application prospect.

Description

technical field [0001] The present invention relates to a targeting chitosan nano-silver gel and its preparation method and application, in particular to a surface-coated hyaluronic acid, imidazole cross-linked chitosan nano-silver gel antibacterial and anticancer material The material and its preparation method and application belong to the technical field of biomedicine. Background technique [0002] Nanogels have the advantages of high stability, good biocompatibility, and large drug loading capacity, which can prevent drug degradation and clearance from the circulatory system. Loading various molecules such as deoxyribonucleic acid, ribonucleic acid, protein, and inorganic materials in the nanogel structure helps to improve the residence time of drugs in the systemic circulation and their biocompatibility, so nanogels can be used as a new type of delivery carrier. [0003] Metal-based drugs such as silver nanoparticles have unique structures and functions. By acting on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K33/38A61K47/36A61K47/54A61P31/04A61P31/10A61P31/12A61P35/00
CPCA61K9/06A61K47/36A61K47/545A61K33/38A61P35/00A61P31/10A61P31/12A61P31/04Y02A50/30
Inventor 张树彪陈会英范雪枫蓝浩铭崔韶晖
Owner DALIAN NATIONALITIES UNIVERSITY
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