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Method for detecting content of anti-moxa drug by ultrasonic-assisted ionic liquid dispersion liquid-liquid microextraction HPLC method

An ionic liquid and dispersion liquid-liquid technology, applied in the field of drug analysis, can solve problems such as increasing the consumption of organic reagents

Active Publication Date: 2020-11-27
YANGZHOU POLYTECHNIC INST
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  • Description
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Problems solved by technology

Use of these reagents increases organic reagent consumption

Method used

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  • Method for detecting content of anti-moxa drug by ultrasonic-assisted ionic liquid dispersion liquid-liquid microextraction HPLC method
  • Method for detecting content of anti-moxa drug by ultrasonic-assisted ionic liquid dispersion liquid-liquid microextraction HPLC method
  • Method for detecting content of anti-moxa drug by ultrasonic-assisted ionic liquid dispersion liquid-liquid microextraction HPLC method

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Experimental program
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Embodiment Construction

[0017] 1.1 Instruments

[0018] Ultimate 3000 high performance liquid chromatograph (Dianyang, USA), KQ3200 ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), L2-4K desktop low-speed centrifuge (Hunan Kecheng Instrument Equipment Co., Ltd.), FE28 pH meter (Switzerland Mette Le Toledo).

[0019] 1.2 Reagents

[0020] ACC007 standard substance (content 99.7%) and tablets (labeled amount 75mg / tablet) were provided by Jiangsu Aidi Pharmaceutical Co., Ltd. Ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate [C 8 mimPF 6 ], 1-butyl-3-methylimidazolium hexafluorophosphate [C 4 mimPF 6 ], 1-hexyl-3-methylimidazolium hexafluorophosphate [C 6 mimPF 6 ] were purchased from Shanghai Dibo Biotechnology Co., Ltd. Ethanol, methanol, glacial acetic acid, and sodium acetate were purchased from Sinopharm Group. Methanol was of chromatographic grade, and the others were of analytical grade.

[0021] 1.3 Solution preparation

[0022] 0.10mg / mL ACC007 standard solutio...

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Abstract

The invention relates to a method for detecting the content of an anti-moxa drug by an ultrasonic-assisted ionic liquid dispersion liquid-liquid microextraction HPLC (High Performance Liquid Chromatography) method. Under the assistance of ultrasonic waves, fine liquid drops can be formed and dispersed in a sample solution without a dispersing agent, namely hydrophobic ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate ([C8mimPF6]), so that ACC007 is effectively extracted and analyzed, and the extraction rate is over 94.0%. Experimental conditions such as the type and the dosage of anextraction agent, the extraction time, the extraction temperature, the pH value of a solution, the cooling time and the centrifugal time are optimized. Under the optimization conditions, the linear range of a standard working curve Y = 10.735 x-0. 328 (r = 0.9994) is 0.50-30.0 microgram / mL, the detection limits are 0.072 microgram / mL (tablets) and 0.10 microgram / mL (serum) respectively, and the adding standard recovery rate is 90.5%-103.4%.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for detecting the content of anti-AIDS drugs by ultrasonic-assisted ionic liquid dispersion liquid-liquid microextraction HPLC method. Background technique [0002] AIDS, also known as acquired immunodeficiency syndrome, is a highly lethal disease caused by human immunodeficiency virus infection. At present, there are more than 30 kinds of drugs in 6 categories in the world for the treatment of AIDS, namely nucleoside reverse transcriptase, non-nucleoside reverse transcriptase, protease, integrase, fusion enzyme and CCR5 inhibitor. ACC007 belongs to the third-generation oral non-nucleoside reverse transcriptase inhibitor, its structure: Chinese name is 3-{[3-ethyl-2,6-dioxo-5-(propyl-2-yl)-1,2,3,6-tetrahydropyrimidin-4-yl]carbonyl}-5 -Toluonitrile. As a new class of anti-AIDS drugs, ACC007 has excellent antiviral activity and good safety. There are few reports on the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 龚爱琴钱琛刘玉婷
Owner YANGZHOU POLYTECHNIC INST
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